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Rescuing apoptotic neurons in Alzheimer's disease using wheat germ agglutinin-conjugated and cardiolipin-conjugated liposomes with encapsulated nerve growth factor and curcumin.

Kuo YC, Lin CC - Int J Nanomedicine (2015)

Bottom Line: An increase in the CL mole percentage in lipids increased the liposomal diameter, absolute zeta potential value, entrapment efficiency of NGF and CUR, release of NGF, biocompatibility, and viability of SK-N-MC cells with Aβ(1-42), but decreased the atomic ratio of nitrogen to phosphorus and release of CUR.In addition, an increase in the WGA concentration for grafting enhanced the liposomal diameter, atomic ratio of nitrogen to phosphorus, and permeability of NGF and CUR across the blood-brain barrier, but reduced the absolute zeta potential value and biocompatibility.WGA-CL-liposomes carrying NGF and CUR could be promising colloidal delivery carriers for future clinical application in targeting the blood-brain barrier and inhibiting neurotoxicity.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemical Engineering, National Chung Cheng University, Chia-Yi, Taiwan, Republic of China.

ABSTRACT
Liposomes with cardiolipin (CL) and wheat germ agglutinin (WGA) were developed to permeate the blood-brain barrier and treat Alzheimer's disease. WGA-conjugated and CL-incorporated liposomes (WGA-CL-liposomes) were used to transport nerve growth factor (NGF) and curcumin (CUR) across a monolayer of human brain-microvascular endothelial cells regulated by human astrocytes and to protect SK-N-MC cells against apoptosis induced by β-amyloid1-42 (Aβ(1-42)) fibrils. An increase in the CL mole percentage in lipids increased the liposomal diameter, absolute zeta potential value, entrapment efficiency of NGF and CUR, release of NGF, biocompatibility, and viability of SK-N-MC cells with Aβ(1-42), but decreased the atomic ratio of nitrogen to phosphorus and release of CUR. In addition, an increase in the WGA concentration for grafting enhanced the liposomal diameter, atomic ratio of nitrogen to phosphorus, and permeability of NGF and CUR across the blood-brain barrier, but reduced the absolute zeta potential value and biocompatibility. WGA-CL-liposomes carrying NGF and CUR could be promising colloidal delivery carriers for future clinical application in targeting the blood-brain barrier and inhibiting neurotoxicity.

No MeSH data available.


Related in: MedlinePlus

Micrographs of WGA-CL-NGF-CUR-liposomes.Notes: (A–H) Transmission electron microscopic images. (I–L) Scanning electron microscopic images. (A, I) rCL =0%; (B, J) rCL =5%; (C, K) rCL =10%; (D, L) rCL =20%; (E) CWGA =2.5 mg/mL and rCL =10%; (F) CWGA =2.5 mg/mL and rCL =20%; (G) CWGA =5 mg/mL and rCL =10%; (H) CWGA =5 mg/mL and rCL =20%.Abbreviations:rCL, CL mole percentage in lipids (%); CWGA, WGA concentration in grafting medium (mg/mL); CL, cardiolipin; CUR, curcumin; NGF, nerve growth factor; WGA, wheat germ agglutinin.
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f3-ijn-10-2653: Micrographs of WGA-CL-NGF-CUR-liposomes.Notes: (A–H) Transmission electron microscopic images. (I–L) Scanning electron microscopic images. (A, I) rCL =0%; (B, J) rCL =5%; (C, K) rCL =10%; (D, L) rCL =20%; (E) CWGA =2.5 mg/mL and rCL =10%; (F) CWGA =2.5 mg/mL and rCL =20%; (G) CWGA =5 mg/mL and rCL =10%; (H) CWGA =5 mg/mL and rCL =20%.Abbreviations:rCL, CL mole percentage in lipids (%); CWGA, WGA concentration in grafting medium (mg/mL); CL, cardiolipin; CUR, curcumin; NGF, nerve growth factor; WGA, wheat germ agglutinin.

Mentions: Figure 3 shows the structure of the WGA-CL-NGF-CUR-liposomes. As indicated in this figure, the particle diameter was about 110–170 nm with appropriate polydispersity, which is consistent with the data shown in Figure 2. As seen in Figure 3A–D, typical bilayer vesicles were obtained. This morphology is comparable with the geometry of liposomes comprising 1,2-dimyristoyl-sn-glycero-3-phosphoethanolamine-N-[poly(ethylene glycol)-2000], DPPC, cholesterol, and palmitic acid.30 As indicated in Figure 3E–H, a higher WGA concentration yielded a thicker and rougher dark external layer of WGA-CL-NGF-CUR-liposomes. This was because conjugated WGA could extend the exterior of the liposomes. As shown in Figure 3I–L, spherical CL-NGF-CUR-liposomes with a smooth surface were observed, which was quite similar to liposomes comprising DPPC and 1,2-distearoyl-sn-glycero-3-phosphocholine.31


Rescuing apoptotic neurons in Alzheimer's disease using wheat germ agglutinin-conjugated and cardiolipin-conjugated liposomes with encapsulated nerve growth factor and curcumin.

Kuo YC, Lin CC - Int J Nanomedicine (2015)

Micrographs of WGA-CL-NGF-CUR-liposomes.Notes: (A–H) Transmission electron microscopic images. (I–L) Scanning electron microscopic images. (A, I) rCL =0%; (B, J) rCL =5%; (C, K) rCL =10%; (D, L) rCL =20%; (E) CWGA =2.5 mg/mL and rCL =10%; (F) CWGA =2.5 mg/mL and rCL =20%; (G) CWGA =5 mg/mL and rCL =10%; (H) CWGA =5 mg/mL and rCL =20%.Abbreviations:rCL, CL mole percentage in lipids (%); CWGA, WGA concentration in grafting medium (mg/mL); CL, cardiolipin; CUR, curcumin; NGF, nerve growth factor; WGA, wheat germ agglutinin.
© Copyright Policy
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4388084&req=5

f3-ijn-10-2653: Micrographs of WGA-CL-NGF-CUR-liposomes.Notes: (A–H) Transmission electron microscopic images. (I–L) Scanning electron microscopic images. (A, I) rCL =0%; (B, J) rCL =5%; (C, K) rCL =10%; (D, L) rCL =20%; (E) CWGA =2.5 mg/mL and rCL =10%; (F) CWGA =2.5 mg/mL and rCL =20%; (G) CWGA =5 mg/mL and rCL =10%; (H) CWGA =5 mg/mL and rCL =20%.Abbreviations:rCL, CL mole percentage in lipids (%); CWGA, WGA concentration in grafting medium (mg/mL); CL, cardiolipin; CUR, curcumin; NGF, nerve growth factor; WGA, wheat germ agglutinin.
Mentions: Figure 3 shows the structure of the WGA-CL-NGF-CUR-liposomes. As indicated in this figure, the particle diameter was about 110–170 nm with appropriate polydispersity, which is consistent with the data shown in Figure 2. As seen in Figure 3A–D, typical bilayer vesicles were obtained. This morphology is comparable with the geometry of liposomes comprising 1,2-dimyristoyl-sn-glycero-3-phosphoethanolamine-N-[poly(ethylene glycol)-2000], DPPC, cholesterol, and palmitic acid.30 As indicated in Figure 3E–H, a higher WGA concentration yielded a thicker and rougher dark external layer of WGA-CL-NGF-CUR-liposomes. This was because conjugated WGA could extend the exterior of the liposomes. As shown in Figure 3I–L, spherical CL-NGF-CUR-liposomes with a smooth surface were observed, which was quite similar to liposomes comprising DPPC and 1,2-distearoyl-sn-glycero-3-phosphocholine.31

Bottom Line: An increase in the CL mole percentage in lipids increased the liposomal diameter, absolute zeta potential value, entrapment efficiency of NGF and CUR, release of NGF, biocompatibility, and viability of SK-N-MC cells with Aβ(1-42), but decreased the atomic ratio of nitrogen to phosphorus and release of CUR.In addition, an increase in the WGA concentration for grafting enhanced the liposomal diameter, atomic ratio of nitrogen to phosphorus, and permeability of NGF and CUR across the blood-brain barrier, but reduced the absolute zeta potential value and biocompatibility.WGA-CL-liposomes carrying NGF and CUR could be promising colloidal delivery carriers for future clinical application in targeting the blood-brain barrier and inhibiting neurotoxicity.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemical Engineering, National Chung Cheng University, Chia-Yi, Taiwan, Republic of China.

ABSTRACT
Liposomes with cardiolipin (CL) and wheat germ agglutinin (WGA) were developed to permeate the blood-brain barrier and treat Alzheimer's disease. WGA-conjugated and CL-incorporated liposomes (WGA-CL-liposomes) were used to transport nerve growth factor (NGF) and curcumin (CUR) across a monolayer of human brain-microvascular endothelial cells regulated by human astrocytes and to protect SK-N-MC cells against apoptosis induced by β-amyloid1-42 (Aβ(1-42)) fibrils. An increase in the CL mole percentage in lipids increased the liposomal diameter, absolute zeta potential value, entrapment efficiency of NGF and CUR, release of NGF, biocompatibility, and viability of SK-N-MC cells with Aβ(1-42), but decreased the atomic ratio of nitrogen to phosphorus and release of CUR. In addition, an increase in the WGA concentration for grafting enhanced the liposomal diameter, atomic ratio of nitrogen to phosphorus, and permeability of NGF and CUR across the blood-brain barrier, but reduced the absolute zeta potential value and biocompatibility. WGA-CL-liposomes carrying NGF and CUR could be promising colloidal delivery carriers for future clinical application in targeting the blood-brain barrier and inhibiting neurotoxicity.

No MeSH data available.


Related in: MedlinePlus