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Rescuing apoptotic neurons in Alzheimer's disease using wheat germ agglutinin-conjugated and cardiolipin-conjugated liposomes with encapsulated nerve growth factor and curcumin.

Kuo YC, Lin CC - Int J Nanomedicine (2015)

Bottom Line: An increase in the CL mole percentage in lipids increased the liposomal diameter, absolute zeta potential value, entrapment efficiency of NGF and CUR, release of NGF, biocompatibility, and viability of SK-N-MC cells with Aβ(1-42), but decreased the atomic ratio of nitrogen to phosphorus and release of CUR.In addition, an increase in the WGA concentration for grafting enhanced the liposomal diameter, atomic ratio of nitrogen to phosphorus, and permeability of NGF and CUR across the blood-brain barrier, but reduced the absolute zeta potential value and biocompatibility.WGA-CL-liposomes carrying NGF and CUR could be promising colloidal delivery carriers for future clinical application in targeting the blood-brain barrier and inhibiting neurotoxicity.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemical Engineering, National Chung Cheng University, Chia-Yi, Taiwan, Republic of China.

ABSTRACT
Liposomes with cardiolipin (CL) and wheat germ agglutinin (WGA) were developed to permeate the blood-brain barrier and treat Alzheimer's disease. WGA-conjugated and CL-incorporated liposomes (WGA-CL-liposomes) were used to transport nerve growth factor (NGF) and curcumin (CUR) across a monolayer of human brain-microvascular endothelial cells regulated by human astrocytes and to protect SK-N-MC cells against apoptosis induced by β-amyloid1-42 (Aβ(1-42)) fibrils. An increase in the CL mole percentage in lipids increased the liposomal diameter, absolute zeta potential value, entrapment efficiency of NGF and CUR, release of NGF, biocompatibility, and viability of SK-N-MC cells with Aβ(1-42), but decreased the atomic ratio of nitrogen to phosphorus and release of CUR. In addition, an increase in the WGA concentration for grafting enhanced the liposomal diameter, atomic ratio of nitrogen to phosphorus, and permeability of NGF and CUR across the blood-brain barrier, but reduced the absolute zeta potential value and biocompatibility. WGA-CL-liposomes carrying NGF and CUR could be promising colloidal delivery carriers for future clinical application in targeting the blood-brain barrier and inhibiting neurotoxicity.

No MeSH data available.


Related in: MedlinePlus

Effect of CL mole percentage on the average diameter of WGA-CL-NGF-CUR-liposomes. (○) CL-liposome; (•) CL-NGF-liposome; (□) WGA-CL-liposome; (△) CL-CUR-liposome; (⋄) WGA-CL-NGF-CUR-liposome.Note:CWGA =5 mg/mL (n=3).Abbreviations:CWGA, WGA concentration in grafting medium (mg/mL); rCL, CL mole percentage in lipids (%); D, average diameter of WGA-CL-NGF-CUR-liposomes (nm); CL, cardiolipin; CUR, curcumin; NGF, nerve growth factor; WGA, wheat germ agglutinin.
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f2-ijn-10-2653: Effect of CL mole percentage on the average diameter of WGA-CL-NGF-CUR-liposomes. (○) CL-liposome; (•) CL-NGF-liposome; (□) WGA-CL-liposome; (△) CL-CUR-liposome; (⋄) WGA-CL-NGF-CUR-liposome.Note:CWGA =5 mg/mL (n=3).Abbreviations:CWGA, WGA concentration in grafting medium (mg/mL); rCL, CL mole percentage in lipids (%); D, average diameter of WGA-CL-NGF-CUR-liposomes (nm); CL, cardiolipin; CUR, curcumin; NGF, nerve growth factor; WGA, wheat germ agglutinin.

Mentions: Figure 2 shows the average particle diameter of the WGA-CL-NGF-CUR-liposomes. As indicated in this figure, an increase in the CL mole percentage enlarged the liposomes from 105 nm to 165 nm, indicating a significant increase in diameter. In addition, incorporation of CUR and WGA increased the liposomal diameter. This was because the ingredients of CL and CUR might extend the bilayer of WGA-CL-NGF-CUR-liposomes. However, entrapment of NGF did not apparently alter the liposomal diameter. This was mainly because the hydrophilic NGF was entrapped in the aqueous core of the CL-NGF-liposomes. Thus, NGF could not affect the surface tension or the bilayer or the liposome size. In a study on electrokinetic capillary chromatography of liposomes, an increase in the CL mole percentage to 10% in lipids primarily comprising 1-palmitoyl-2-oleyl-sn-glycerophosphatidylcholine increased the particle size.25 It has also been observed that inclusion of CUR in the lipid bilayer of sphingomyelin and cholesterol increased the particle size.26 Moreover, modification with transferrin on the particle surface was found to increase the surface layer thickness of liposomes comprising L-α-soy phosphatidyl choline, stearylamine, and cholesterol.27


Rescuing apoptotic neurons in Alzheimer's disease using wheat germ agglutinin-conjugated and cardiolipin-conjugated liposomes with encapsulated nerve growth factor and curcumin.

Kuo YC, Lin CC - Int J Nanomedicine (2015)

Effect of CL mole percentage on the average diameter of WGA-CL-NGF-CUR-liposomes. (○) CL-liposome; (•) CL-NGF-liposome; (□) WGA-CL-liposome; (△) CL-CUR-liposome; (⋄) WGA-CL-NGF-CUR-liposome.Note:CWGA =5 mg/mL (n=3).Abbreviations:CWGA, WGA concentration in grafting medium (mg/mL); rCL, CL mole percentage in lipids (%); D, average diameter of WGA-CL-NGF-CUR-liposomes (nm); CL, cardiolipin; CUR, curcumin; NGF, nerve growth factor; WGA, wheat germ agglutinin.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4388084&req=5

f2-ijn-10-2653: Effect of CL mole percentage on the average diameter of WGA-CL-NGF-CUR-liposomes. (○) CL-liposome; (•) CL-NGF-liposome; (□) WGA-CL-liposome; (△) CL-CUR-liposome; (⋄) WGA-CL-NGF-CUR-liposome.Note:CWGA =5 mg/mL (n=3).Abbreviations:CWGA, WGA concentration in grafting medium (mg/mL); rCL, CL mole percentage in lipids (%); D, average diameter of WGA-CL-NGF-CUR-liposomes (nm); CL, cardiolipin; CUR, curcumin; NGF, nerve growth factor; WGA, wheat germ agglutinin.
Mentions: Figure 2 shows the average particle diameter of the WGA-CL-NGF-CUR-liposomes. As indicated in this figure, an increase in the CL mole percentage enlarged the liposomes from 105 nm to 165 nm, indicating a significant increase in diameter. In addition, incorporation of CUR and WGA increased the liposomal diameter. This was because the ingredients of CL and CUR might extend the bilayer of WGA-CL-NGF-CUR-liposomes. However, entrapment of NGF did not apparently alter the liposomal diameter. This was mainly because the hydrophilic NGF was entrapped in the aqueous core of the CL-NGF-liposomes. Thus, NGF could not affect the surface tension or the bilayer or the liposome size. In a study on electrokinetic capillary chromatography of liposomes, an increase in the CL mole percentage to 10% in lipids primarily comprising 1-palmitoyl-2-oleyl-sn-glycerophosphatidylcholine increased the particle size.25 It has also been observed that inclusion of CUR in the lipid bilayer of sphingomyelin and cholesterol increased the particle size.26 Moreover, modification with transferrin on the particle surface was found to increase the surface layer thickness of liposomes comprising L-α-soy phosphatidyl choline, stearylamine, and cholesterol.27

Bottom Line: An increase in the CL mole percentage in lipids increased the liposomal diameter, absolute zeta potential value, entrapment efficiency of NGF and CUR, release of NGF, biocompatibility, and viability of SK-N-MC cells with Aβ(1-42), but decreased the atomic ratio of nitrogen to phosphorus and release of CUR.In addition, an increase in the WGA concentration for grafting enhanced the liposomal diameter, atomic ratio of nitrogen to phosphorus, and permeability of NGF and CUR across the blood-brain barrier, but reduced the absolute zeta potential value and biocompatibility.WGA-CL-liposomes carrying NGF and CUR could be promising colloidal delivery carriers for future clinical application in targeting the blood-brain barrier and inhibiting neurotoxicity.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemical Engineering, National Chung Cheng University, Chia-Yi, Taiwan, Republic of China.

ABSTRACT
Liposomes with cardiolipin (CL) and wheat germ agglutinin (WGA) were developed to permeate the blood-brain barrier and treat Alzheimer's disease. WGA-conjugated and CL-incorporated liposomes (WGA-CL-liposomes) were used to transport nerve growth factor (NGF) and curcumin (CUR) across a monolayer of human brain-microvascular endothelial cells regulated by human astrocytes and to protect SK-N-MC cells against apoptosis induced by β-amyloid1-42 (Aβ(1-42)) fibrils. An increase in the CL mole percentage in lipids increased the liposomal diameter, absolute zeta potential value, entrapment efficiency of NGF and CUR, release of NGF, biocompatibility, and viability of SK-N-MC cells with Aβ(1-42), but decreased the atomic ratio of nitrogen to phosphorus and release of CUR. In addition, an increase in the WGA concentration for grafting enhanced the liposomal diameter, atomic ratio of nitrogen to phosphorus, and permeability of NGF and CUR across the blood-brain barrier, but reduced the absolute zeta potential value and biocompatibility. WGA-CL-liposomes carrying NGF and CUR could be promising colloidal delivery carriers for future clinical application in targeting the blood-brain barrier and inhibiting neurotoxicity.

No MeSH data available.


Related in: MedlinePlus