Limits...
Rescuing apoptotic neurons in Alzheimer's disease using wheat germ agglutinin-conjugated and cardiolipin-conjugated liposomes with encapsulated nerve growth factor and curcumin.

Kuo YC, Lin CC - Int J Nanomedicine (2015)

Bottom Line: An increase in the CL mole percentage in lipids increased the liposomal diameter, absolute zeta potential value, entrapment efficiency of NGF and CUR, release of NGF, biocompatibility, and viability of SK-N-MC cells with Aβ(1-42), but decreased the atomic ratio of nitrogen to phosphorus and release of CUR.In addition, an increase in the WGA concentration for grafting enhanced the liposomal diameter, atomic ratio of nitrogen to phosphorus, and permeability of NGF and CUR across the blood-brain barrier, but reduced the absolute zeta potential value and biocompatibility.WGA-CL-liposomes carrying NGF and CUR could be promising colloidal delivery carriers for future clinical application in targeting the blood-brain barrier and inhibiting neurotoxicity.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemical Engineering, National Chung Cheng University, Chia-Yi, Taiwan, Republic of China.

ABSTRACT
Liposomes with cardiolipin (CL) and wheat germ agglutinin (WGA) were developed to permeate the blood-brain barrier and treat Alzheimer's disease. WGA-conjugated and CL-incorporated liposomes (WGA-CL-liposomes) were used to transport nerve growth factor (NGF) and curcumin (CUR) across a monolayer of human brain-microvascular endothelial cells regulated by human astrocytes and to protect SK-N-MC cells against apoptosis induced by β-amyloid1-42 (Aβ(1-42)) fibrils. An increase in the CL mole percentage in lipids increased the liposomal diameter, absolute zeta potential value, entrapment efficiency of NGF and CUR, release of NGF, biocompatibility, and viability of SK-N-MC cells with Aβ(1-42), but decreased the atomic ratio of nitrogen to phosphorus and release of CUR. In addition, an increase in the WGA concentration for grafting enhanced the liposomal diameter, atomic ratio of nitrogen to phosphorus, and permeability of NGF and CUR across the blood-brain barrier, but reduced the absolute zeta potential value and biocompatibility. WGA-CL-liposomes carrying NGF and CUR could be promising colloidal delivery carriers for future clinical application in targeting the blood-brain barrier and inhibiting neurotoxicity.

No MeSH data available.


Related in: MedlinePlus

Schematic illustration of the structure of a WGA-CL-NGF-CUR-liposome.Abbreviations: DSPE-PEG(2000), 1,2-dipalmitoyl-sn-glycero-3-phosphoethanol-amine-N-[methoxy(polyethylene glycol)-2000]; DSPE-PEG(2000)-CA, 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[carboxy(polyethylene glycol)-2000]; DPPC, 1,2-dipalmitoyl-sn-glycero-3-phosphocholine; CL, cardiolipin; CUR, curcumin; NGF, nerve growth factor; WGA, wheat germ agglutinin.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4388084&req=5

f1-ijn-10-2653: Schematic illustration of the structure of a WGA-CL-NGF-CUR-liposome.Abbreviations: DSPE-PEG(2000), 1,2-dipalmitoyl-sn-glycero-3-phosphoethanol-amine-N-[methoxy(polyethylene glycol)-2000]; DSPE-PEG(2000)-CA, 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[carboxy(polyethylene glycol)-2000]; DPPC, 1,2-dipalmitoyl-sn-glycero-3-phosphocholine; CL, cardiolipin; CUR, curcumin; NGF, nerve growth factor; WGA, wheat germ agglutinin.

Mentions: CL-NGF-CUR-liposomes were suspended in DPBS containing 20% (w/v) 2-(N-morpholino)ethanesulfonic acid at 80 rpm and mixed with 1 mM 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide and 1.5 mM N-hydroxysuccinimide at 80 rpm and 25°C for 1.5 hours. The suspension containing CL-NGF-CUR-liposomes with functionalized carboxyl groups was mixed with WGA 2.5 or 5 mg/mL at 80 rpm and 25°C for 12 hours. Next, the suspension containing 3 mL of WGA-conjugated CL-NGF-CUR-liposomes and 150 mL of DPBS was added to the upright Sephadex G-100 column at 25°C to separate the free WGA from the WGA-CL-NGF-CUR-liposomes in every 2 mL effluent. The quantity of free WGA was assayed using a QuantiPro bicinchoninic acid assay kit and analyzed using the ELISA spectrophotometer at 562 nm. The efficiency of WGA grafting on WGA-CL-NGF-CUR-liposomes, GEWGA, was defined as GEWGA (%) = [(Wt,WGA − Wf,WGA)/Wt,WGA] ×100%, where Wt,WGA and Wf,WGA are the weight of total WGA and the weight of free WGA after gel chromatography, respectively. Figure 1 shows a schematic illustration of a WGA-CL-NGF-CUR-liposome.


Rescuing apoptotic neurons in Alzheimer's disease using wheat germ agglutinin-conjugated and cardiolipin-conjugated liposomes with encapsulated nerve growth factor and curcumin.

Kuo YC, Lin CC - Int J Nanomedicine (2015)

Schematic illustration of the structure of a WGA-CL-NGF-CUR-liposome.Abbreviations: DSPE-PEG(2000), 1,2-dipalmitoyl-sn-glycero-3-phosphoethanol-amine-N-[methoxy(polyethylene glycol)-2000]; DSPE-PEG(2000)-CA, 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[carboxy(polyethylene glycol)-2000]; DPPC, 1,2-dipalmitoyl-sn-glycero-3-phosphocholine; CL, cardiolipin; CUR, curcumin; NGF, nerve growth factor; WGA, wheat germ agglutinin.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4388084&req=5

f1-ijn-10-2653: Schematic illustration of the structure of a WGA-CL-NGF-CUR-liposome.Abbreviations: DSPE-PEG(2000), 1,2-dipalmitoyl-sn-glycero-3-phosphoethanol-amine-N-[methoxy(polyethylene glycol)-2000]; DSPE-PEG(2000)-CA, 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[carboxy(polyethylene glycol)-2000]; DPPC, 1,2-dipalmitoyl-sn-glycero-3-phosphocholine; CL, cardiolipin; CUR, curcumin; NGF, nerve growth factor; WGA, wheat germ agglutinin.
Mentions: CL-NGF-CUR-liposomes were suspended in DPBS containing 20% (w/v) 2-(N-morpholino)ethanesulfonic acid at 80 rpm and mixed with 1 mM 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide and 1.5 mM N-hydroxysuccinimide at 80 rpm and 25°C for 1.5 hours. The suspension containing CL-NGF-CUR-liposomes with functionalized carboxyl groups was mixed with WGA 2.5 or 5 mg/mL at 80 rpm and 25°C for 12 hours. Next, the suspension containing 3 mL of WGA-conjugated CL-NGF-CUR-liposomes and 150 mL of DPBS was added to the upright Sephadex G-100 column at 25°C to separate the free WGA from the WGA-CL-NGF-CUR-liposomes in every 2 mL effluent. The quantity of free WGA was assayed using a QuantiPro bicinchoninic acid assay kit and analyzed using the ELISA spectrophotometer at 562 nm. The efficiency of WGA grafting on WGA-CL-NGF-CUR-liposomes, GEWGA, was defined as GEWGA (%) = [(Wt,WGA − Wf,WGA)/Wt,WGA] ×100%, where Wt,WGA and Wf,WGA are the weight of total WGA and the weight of free WGA after gel chromatography, respectively. Figure 1 shows a schematic illustration of a WGA-CL-NGF-CUR-liposome.

Bottom Line: An increase in the CL mole percentage in lipids increased the liposomal diameter, absolute zeta potential value, entrapment efficiency of NGF and CUR, release of NGF, biocompatibility, and viability of SK-N-MC cells with Aβ(1-42), but decreased the atomic ratio of nitrogen to phosphorus and release of CUR.In addition, an increase in the WGA concentration for grafting enhanced the liposomal diameter, atomic ratio of nitrogen to phosphorus, and permeability of NGF and CUR across the blood-brain barrier, but reduced the absolute zeta potential value and biocompatibility.WGA-CL-liposomes carrying NGF and CUR could be promising colloidal delivery carriers for future clinical application in targeting the blood-brain barrier and inhibiting neurotoxicity.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemical Engineering, National Chung Cheng University, Chia-Yi, Taiwan, Republic of China.

ABSTRACT
Liposomes with cardiolipin (CL) and wheat germ agglutinin (WGA) were developed to permeate the blood-brain barrier and treat Alzheimer's disease. WGA-conjugated and CL-incorporated liposomes (WGA-CL-liposomes) were used to transport nerve growth factor (NGF) and curcumin (CUR) across a monolayer of human brain-microvascular endothelial cells regulated by human astrocytes and to protect SK-N-MC cells against apoptosis induced by β-amyloid1-42 (Aβ(1-42)) fibrils. An increase in the CL mole percentage in lipids increased the liposomal diameter, absolute zeta potential value, entrapment efficiency of NGF and CUR, release of NGF, biocompatibility, and viability of SK-N-MC cells with Aβ(1-42), but decreased the atomic ratio of nitrogen to phosphorus and release of CUR. In addition, an increase in the WGA concentration for grafting enhanced the liposomal diameter, atomic ratio of nitrogen to phosphorus, and permeability of NGF and CUR across the blood-brain barrier, but reduced the absolute zeta potential value and biocompatibility. WGA-CL-liposomes carrying NGF and CUR could be promising colloidal delivery carriers for future clinical application in targeting the blood-brain barrier and inhibiting neurotoxicity.

No MeSH data available.


Related in: MedlinePlus