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Enhanced magnetic resonance imaging and staining of cancer cells using ferrimagnetic H-ferritin nanoparticles with increasing core size.

Cai Y, Cao C, He X, Yang C, Tian L, Zhu R, Pan Y - Int J Nanomedicine (2015)

Bottom Line: In vitro MRI of cell pellets after M-HFn labeling was performed at 7 T.Iron uptake of cells was analyzed by Prussian blue staining and inductively coupled plasma mass spectrometry.The saturation magnetization (M(s)), relaxivity, and peroxidase-like activity of synthesized M-HFn nanoparticles were monotonously increased with the size of ferrimagnetic cores.

View Article: PubMed Central - PubMed

Affiliation: France-China Bio-Mineralization and Nano-Structures Laboratory, Key Laboratory of the Earth and Planetary Physics, Institute of Geology and Geophysics, Chinese Academy of Sciences, People's Republic of China ; Paleomagnetism and Geochronology Laboratory, Key Laboratory of the Earth and Planetary Physics, Institute of Geology and Geophysics, Chinese Academy of Sciences, People's Republic of China ; University of Chinese Academy of Sciences, Beijing, People's Republic of China.

ABSTRACT

Purpose: This study is to demonstrate the nanoscale size effect of ferrimagnetic H-ferritin (M-HFn) nanoparticles on magnetic properties, relaxivity, enzyme mimetic activities, and application in magnetic resonance imaging (MRI) and immunohistochemical staining of cancer cells.

Materials and methods: M-HFn nanoparticles with different sizes of magnetite cores in the range of 2.7-5.3 nm were synthesized through loading different amounts of iron into recombinant human H chain ferritin (HFn) shells. Core size, crystallinity, and magnetic properties of those M-HFn nanoparticles were analyzed by transmission electron microscope and low-temperature magnetic measurements. The MDA-MB-231 cancer cells were incubated with synthesized M-HFn nanoparticles for 24 hours in Dulbecco's Modified Eagle's Medium. In vitro MRI of cell pellets after M-HFn labeling was performed at 7 T. Iron uptake of cells was analyzed by Prussian blue staining and inductively coupled plasma mass spectrometry. Immunohistochemical staining by using the peroxidase-like activity of M-HFn nanoparticles was carried out on MDA-MB-231 tumor tissue paraffin sections.

Results: The saturation magnetization (M(s)), relaxivity, and peroxidase-like activity of synthesized M-HFn nanoparticles were monotonously increased with the size of ferrimagnetic cores. The M-HFn nanoparticles with the largest core size of 5.3 nm exhibit the strongest saturation magnetization, the highest peroxidase activity in immunohistochemical staining, and the highest r2 of 321 mM(-1) s(-1), allowing to detect MDA-MB-231 breast cancer cells as low as 10(4) cells mL(-1).

Conclusion: The magnetic properties, relaxivity, and peroxidase-like activity of M-HFn nanoparticles are size dependent, which indicates that M-HFn nanoparticles with larger magnetite core can significantly enhance performance in MRI and staining of cancer cells.

No MeSH data available.


Related in: MedlinePlus

Iron uptake analysis of MDA-MB-231 tumor cells incubated with M-HFn nanoparticles.Notes: Prussian blue staining of MDA-MB-231 tumor cells incubated for 24 hours with (A) no nanoparticles, (B) M-HFn1000, (C) M-HFn3000, (D) M-HFn5000, and (E) M-HFn7000. (F) Iron contents in single cell are 0.16 pg cell−1, 0.29 pg cell−1, 0.80 pg cell−1, and 1.23 pg cell−1 after incubation with M-HFn1000, M-HFn3000, M-HFn5000, and M-HFn7000, respectively, for 24 hours (statistical comparison of iron contents in single cell with cell-only yielded P=0.014, 0.002, 0.011, and 0.023 for M-HFn1000, M-HFn3000, M-HFn5000, and M-HFn7000, respectively).Abbreviation: M-HFn, ferrimagnetic H-ferritin.
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f6-ijn-10-2619: Iron uptake analysis of MDA-MB-231 tumor cells incubated with M-HFn nanoparticles.Notes: Prussian blue staining of MDA-MB-231 tumor cells incubated for 24 hours with (A) no nanoparticles, (B) M-HFn1000, (C) M-HFn3000, (D) M-HFn5000, and (E) M-HFn7000. (F) Iron contents in single cell are 0.16 pg cell−1, 0.29 pg cell−1, 0.80 pg cell−1, and 1.23 pg cell−1 after incubation with M-HFn1000, M-HFn3000, M-HFn5000, and M-HFn7000, respectively, for 24 hours (statistical comparison of iron contents in single cell with cell-only yielded P=0.014, 0.002, 0.011, and 0.023 for M-HFn1000, M-HFn3000, M-HFn5000, and M-HFn7000, respectively).Abbreviation: M-HFn, ferrimagnetic H-ferritin.

Mentions: To further identify iron uptaken by cancer cells, we performed Prussian blue staining for cancer cells after incubation with the M-HFn nanoparticles. Results are shown in Figure 6. It can be seen that there is more blue deposition in cancer cells incubated with M-HFn nanoparticles with larger core size. We also assessed the iron content in cancer cells by ICP-MS and found that cancer cells accumulated 0.16 pg cell−1, 0.29 pg cell−1, 0.80 pg cell−1, and 1.23 pg cell−1 of Fe after being incubated with equal amount of M-HFn1000, M-HFn3000, M-HFn5000, and M-HFn7000 for 24 hours, respectively (Figure 6F).


Enhanced magnetic resonance imaging and staining of cancer cells using ferrimagnetic H-ferritin nanoparticles with increasing core size.

Cai Y, Cao C, He X, Yang C, Tian L, Zhu R, Pan Y - Int J Nanomedicine (2015)

Iron uptake analysis of MDA-MB-231 tumor cells incubated with M-HFn nanoparticles.Notes: Prussian blue staining of MDA-MB-231 tumor cells incubated for 24 hours with (A) no nanoparticles, (B) M-HFn1000, (C) M-HFn3000, (D) M-HFn5000, and (E) M-HFn7000. (F) Iron contents in single cell are 0.16 pg cell−1, 0.29 pg cell−1, 0.80 pg cell−1, and 1.23 pg cell−1 after incubation with M-HFn1000, M-HFn3000, M-HFn5000, and M-HFn7000, respectively, for 24 hours (statistical comparison of iron contents in single cell with cell-only yielded P=0.014, 0.002, 0.011, and 0.023 for M-HFn1000, M-HFn3000, M-HFn5000, and M-HFn7000, respectively).Abbreviation: M-HFn, ferrimagnetic H-ferritin.
© Copyright Policy
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4388082&req=5

f6-ijn-10-2619: Iron uptake analysis of MDA-MB-231 tumor cells incubated with M-HFn nanoparticles.Notes: Prussian blue staining of MDA-MB-231 tumor cells incubated for 24 hours with (A) no nanoparticles, (B) M-HFn1000, (C) M-HFn3000, (D) M-HFn5000, and (E) M-HFn7000. (F) Iron contents in single cell are 0.16 pg cell−1, 0.29 pg cell−1, 0.80 pg cell−1, and 1.23 pg cell−1 after incubation with M-HFn1000, M-HFn3000, M-HFn5000, and M-HFn7000, respectively, for 24 hours (statistical comparison of iron contents in single cell with cell-only yielded P=0.014, 0.002, 0.011, and 0.023 for M-HFn1000, M-HFn3000, M-HFn5000, and M-HFn7000, respectively).Abbreviation: M-HFn, ferrimagnetic H-ferritin.
Mentions: To further identify iron uptaken by cancer cells, we performed Prussian blue staining for cancer cells after incubation with the M-HFn nanoparticles. Results are shown in Figure 6. It can be seen that there is more blue deposition in cancer cells incubated with M-HFn nanoparticles with larger core size. We also assessed the iron content in cancer cells by ICP-MS and found that cancer cells accumulated 0.16 pg cell−1, 0.29 pg cell−1, 0.80 pg cell−1, and 1.23 pg cell−1 of Fe after being incubated with equal amount of M-HFn1000, M-HFn3000, M-HFn5000, and M-HFn7000 for 24 hours, respectively (Figure 6F).

Bottom Line: In vitro MRI of cell pellets after M-HFn labeling was performed at 7 T.Iron uptake of cells was analyzed by Prussian blue staining and inductively coupled plasma mass spectrometry.The saturation magnetization (M(s)), relaxivity, and peroxidase-like activity of synthesized M-HFn nanoparticles were monotonously increased with the size of ferrimagnetic cores.

View Article: PubMed Central - PubMed

Affiliation: France-China Bio-Mineralization and Nano-Structures Laboratory, Key Laboratory of the Earth and Planetary Physics, Institute of Geology and Geophysics, Chinese Academy of Sciences, People's Republic of China ; Paleomagnetism and Geochronology Laboratory, Key Laboratory of the Earth and Planetary Physics, Institute of Geology and Geophysics, Chinese Academy of Sciences, People's Republic of China ; University of Chinese Academy of Sciences, Beijing, People's Republic of China.

ABSTRACT

Purpose: This study is to demonstrate the nanoscale size effect of ferrimagnetic H-ferritin (M-HFn) nanoparticles on magnetic properties, relaxivity, enzyme mimetic activities, and application in magnetic resonance imaging (MRI) and immunohistochemical staining of cancer cells.

Materials and methods: M-HFn nanoparticles with different sizes of magnetite cores in the range of 2.7-5.3 nm were synthesized through loading different amounts of iron into recombinant human H chain ferritin (HFn) shells. Core size, crystallinity, and magnetic properties of those M-HFn nanoparticles were analyzed by transmission electron microscope and low-temperature magnetic measurements. The MDA-MB-231 cancer cells were incubated with synthesized M-HFn nanoparticles for 24 hours in Dulbecco's Modified Eagle's Medium. In vitro MRI of cell pellets after M-HFn labeling was performed at 7 T. Iron uptake of cells was analyzed by Prussian blue staining and inductively coupled plasma mass spectrometry. Immunohistochemical staining by using the peroxidase-like activity of M-HFn nanoparticles was carried out on MDA-MB-231 tumor tissue paraffin sections.

Results: The saturation magnetization (M(s)), relaxivity, and peroxidase-like activity of synthesized M-HFn nanoparticles were monotonously increased with the size of ferrimagnetic cores. The M-HFn nanoparticles with the largest core size of 5.3 nm exhibit the strongest saturation magnetization, the highest peroxidase activity in immunohistochemical staining, and the highest r2 of 321 mM(-1) s(-1), allowing to detect MDA-MB-231 breast cancer cells as low as 10(4) cells mL(-1).

Conclusion: The magnetic properties, relaxivity, and peroxidase-like activity of M-HFn nanoparticles are size dependent, which indicates that M-HFn nanoparticles with larger magnetite core can significantly enhance performance in MRI and staining of cancer cells.

No MeSH data available.


Related in: MedlinePlus