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Enhanced magnetic resonance imaging and staining of cancer cells using ferrimagnetic H-ferritin nanoparticles with increasing core size.

Cai Y, Cao C, He X, Yang C, Tian L, Zhu R, Pan Y - Int J Nanomedicine (2015)

Bottom Line: In vitro MRI of cell pellets after M-HFn labeling was performed at 7 T.Iron uptake of cells was analyzed by Prussian blue staining and inductively coupled plasma mass spectrometry.The saturation magnetization (M(s)), relaxivity, and peroxidase-like activity of synthesized M-HFn nanoparticles were monotonously increased with the size of ferrimagnetic cores.

View Article: PubMed Central - PubMed

Affiliation: France-China Bio-Mineralization and Nano-Structures Laboratory, Key Laboratory of the Earth and Planetary Physics, Institute of Geology and Geophysics, Chinese Academy of Sciences, People's Republic of China ; Paleomagnetism and Geochronology Laboratory, Key Laboratory of the Earth and Planetary Physics, Institute of Geology and Geophysics, Chinese Academy of Sciences, People's Republic of China ; University of Chinese Academy of Sciences, Beijing, People's Republic of China.

ABSTRACT

Purpose: This study is to demonstrate the nanoscale size effect of ferrimagnetic H-ferritin (M-HFn) nanoparticles on magnetic properties, relaxivity, enzyme mimetic activities, and application in magnetic resonance imaging (MRI) and immunohistochemical staining of cancer cells.

Materials and methods: M-HFn nanoparticles with different sizes of magnetite cores in the range of 2.7-5.3 nm were synthesized through loading different amounts of iron into recombinant human H chain ferritin (HFn) shells. Core size, crystallinity, and magnetic properties of those M-HFn nanoparticles were analyzed by transmission electron microscope and low-temperature magnetic measurements. The MDA-MB-231 cancer cells were incubated with synthesized M-HFn nanoparticles for 24 hours in Dulbecco's Modified Eagle's Medium. In vitro MRI of cell pellets after M-HFn labeling was performed at 7 T. Iron uptake of cells was analyzed by Prussian blue staining and inductively coupled plasma mass spectrometry. Immunohistochemical staining by using the peroxidase-like activity of M-HFn nanoparticles was carried out on MDA-MB-231 tumor tissue paraffin sections.

Results: The saturation magnetization (M(s)), relaxivity, and peroxidase-like activity of synthesized M-HFn nanoparticles were monotonously increased with the size of ferrimagnetic cores. The M-HFn nanoparticles with the largest core size of 5.3 nm exhibit the strongest saturation magnetization, the highest peroxidase activity in immunohistochemical staining, and the highest r2 of 321 mM(-1) s(-1), allowing to detect MDA-MB-231 breast cancer cells as low as 10(4) cells mL(-1).

Conclusion: The magnetic properties, relaxivity, and peroxidase-like activity of M-HFn nanoparticles are size dependent, which indicates that M-HFn nanoparticles with larger magnetite core can significantly enhance performance in MRI and staining of cancer cells.

No MeSH data available.


Related in: MedlinePlus

MRI of MDA-MB-231 tumor cells incubated with M-HFn nanoparticles.Notes: (A) Evaluation of the efficiency of M-HFn nanoparticles in detecting MDA-MB-231 tumor cells by MRI. T2-weighted MR images of (a) tumor cell-only, and tumor cells incubated with (b) M-HFn1000, (c) M-HFn3000, (d) M-HFn5000, and (e) M-HFn7000. T2 values are 122.5±0.7 ms, 110.9±1.5 ms, 99.1±1.9 ms, 90.0±3.1 ms, and 81.2±2.6 ms (P<0.05, n=3). (B) T2-weighted MR images of tumor cells of different concentrations incubated with M-HFn7000 nanoparticles: (g) 0 cells mL−1, (h) 1×104 cells mL−1, (i) 1×105 cells mL−1, (j) 2×105 cells mL−1, and (k) 3×105 cells mL−1. T2 values are 140.4±3.7 ms, 103.2±0.4 ms, 93.2±0.8 ms, 88.8±0.8 ms, and 81.2±2.6 ms (P<0.05, n=3).Abbreviation: M-HFn, ferrimagnetic H-ferritin; MR, magnetic resonance; MRI, magnetic resonance imaging.
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f5-ijn-10-2619: MRI of MDA-MB-231 tumor cells incubated with M-HFn nanoparticles.Notes: (A) Evaluation of the efficiency of M-HFn nanoparticles in detecting MDA-MB-231 tumor cells by MRI. T2-weighted MR images of (a) tumor cell-only, and tumor cells incubated with (b) M-HFn1000, (c) M-HFn3000, (d) M-HFn5000, and (e) M-HFn7000. T2 values are 122.5±0.7 ms, 110.9±1.5 ms, 99.1±1.9 ms, 90.0±3.1 ms, and 81.2±2.6 ms (P<0.05, n=3). (B) T2-weighted MR images of tumor cells of different concentrations incubated with M-HFn7000 nanoparticles: (g) 0 cells mL−1, (h) 1×104 cells mL−1, (i) 1×105 cells mL−1, (j) 2×105 cells mL−1, and (k) 3×105 cells mL−1. T2 values are 140.4±3.7 ms, 103.2±0.4 ms, 93.2±0.8 ms, 88.8±0.8 ms, and 81.2±2.6 ms (P<0.05, n=3).Abbreviation: M-HFn, ferrimagnetic H-ferritin; MR, magnetic resonance; MRI, magnetic resonance imaging.

Mentions: In vitro MRI of MDA-MB-231 tumor cells after incubation with M-HFn nanoparticles shows obvious dark images compared to cells-only control (Figure 5A). The MDA-MB-231 cancer cells incubated with M-HFn1000, M-HFn3000, M-HFn5000, and M-HFn7000 have T2 values equal to 110.9 ms, 99.1 ms, 90.0 ms, and 81.2 ms, respectively, while the cells-only control (incubated without M-HFn nanoparticles) has a T2 value of 122.5 ms. As shown in Figure 5B, the T2 values of MDA-MB-231 tumor cells with cell densities of 1×104 cells mL−1, 1×105 cells mL−1, 2×105 cells mL−1, and 3×105 cells mL−1 incubated with M-HFn7000 reduce to 73.5%, 66.3%, 63.2%, and 57.8%, respectively, compared with the T2 value of control (0 cells mL−1).


Enhanced magnetic resonance imaging and staining of cancer cells using ferrimagnetic H-ferritin nanoparticles with increasing core size.

Cai Y, Cao C, He X, Yang C, Tian L, Zhu R, Pan Y - Int J Nanomedicine (2015)

MRI of MDA-MB-231 tumor cells incubated with M-HFn nanoparticles.Notes: (A) Evaluation of the efficiency of M-HFn nanoparticles in detecting MDA-MB-231 tumor cells by MRI. T2-weighted MR images of (a) tumor cell-only, and tumor cells incubated with (b) M-HFn1000, (c) M-HFn3000, (d) M-HFn5000, and (e) M-HFn7000. T2 values are 122.5±0.7 ms, 110.9±1.5 ms, 99.1±1.9 ms, 90.0±3.1 ms, and 81.2±2.6 ms (P<0.05, n=3). (B) T2-weighted MR images of tumor cells of different concentrations incubated with M-HFn7000 nanoparticles: (g) 0 cells mL−1, (h) 1×104 cells mL−1, (i) 1×105 cells mL−1, (j) 2×105 cells mL−1, and (k) 3×105 cells mL−1. T2 values are 140.4±3.7 ms, 103.2±0.4 ms, 93.2±0.8 ms, 88.8±0.8 ms, and 81.2±2.6 ms (P<0.05, n=3).Abbreviation: M-HFn, ferrimagnetic H-ferritin; MR, magnetic resonance; MRI, magnetic resonance imaging.
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f5-ijn-10-2619: MRI of MDA-MB-231 tumor cells incubated with M-HFn nanoparticles.Notes: (A) Evaluation of the efficiency of M-HFn nanoparticles in detecting MDA-MB-231 tumor cells by MRI. T2-weighted MR images of (a) tumor cell-only, and tumor cells incubated with (b) M-HFn1000, (c) M-HFn3000, (d) M-HFn5000, and (e) M-HFn7000. T2 values are 122.5±0.7 ms, 110.9±1.5 ms, 99.1±1.9 ms, 90.0±3.1 ms, and 81.2±2.6 ms (P<0.05, n=3). (B) T2-weighted MR images of tumor cells of different concentrations incubated with M-HFn7000 nanoparticles: (g) 0 cells mL−1, (h) 1×104 cells mL−1, (i) 1×105 cells mL−1, (j) 2×105 cells mL−1, and (k) 3×105 cells mL−1. T2 values are 140.4±3.7 ms, 103.2±0.4 ms, 93.2±0.8 ms, 88.8±0.8 ms, and 81.2±2.6 ms (P<0.05, n=3).Abbreviation: M-HFn, ferrimagnetic H-ferritin; MR, magnetic resonance; MRI, magnetic resonance imaging.
Mentions: In vitro MRI of MDA-MB-231 tumor cells after incubation with M-HFn nanoparticles shows obvious dark images compared to cells-only control (Figure 5A). The MDA-MB-231 cancer cells incubated with M-HFn1000, M-HFn3000, M-HFn5000, and M-HFn7000 have T2 values equal to 110.9 ms, 99.1 ms, 90.0 ms, and 81.2 ms, respectively, while the cells-only control (incubated without M-HFn nanoparticles) has a T2 value of 122.5 ms. As shown in Figure 5B, the T2 values of MDA-MB-231 tumor cells with cell densities of 1×104 cells mL−1, 1×105 cells mL−1, 2×105 cells mL−1, and 3×105 cells mL−1 incubated with M-HFn7000 reduce to 73.5%, 66.3%, 63.2%, and 57.8%, respectively, compared with the T2 value of control (0 cells mL−1).

Bottom Line: In vitro MRI of cell pellets after M-HFn labeling was performed at 7 T.Iron uptake of cells was analyzed by Prussian blue staining and inductively coupled plasma mass spectrometry.The saturation magnetization (M(s)), relaxivity, and peroxidase-like activity of synthesized M-HFn nanoparticles were monotonously increased with the size of ferrimagnetic cores.

View Article: PubMed Central - PubMed

Affiliation: France-China Bio-Mineralization and Nano-Structures Laboratory, Key Laboratory of the Earth and Planetary Physics, Institute of Geology and Geophysics, Chinese Academy of Sciences, People's Republic of China ; Paleomagnetism and Geochronology Laboratory, Key Laboratory of the Earth and Planetary Physics, Institute of Geology and Geophysics, Chinese Academy of Sciences, People's Republic of China ; University of Chinese Academy of Sciences, Beijing, People's Republic of China.

ABSTRACT

Purpose: This study is to demonstrate the nanoscale size effect of ferrimagnetic H-ferritin (M-HFn) nanoparticles on magnetic properties, relaxivity, enzyme mimetic activities, and application in magnetic resonance imaging (MRI) and immunohistochemical staining of cancer cells.

Materials and methods: M-HFn nanoparticles with different sizes of magnetite cores in the range of 2.7-5.3 nm were synthesized through loading different amounts of iron into recombinant human H chain ferritin (HFn) shells. Core size, crystallinity, and magnetic properties of those M-HFn nanoparticles were analyzed by transmission electron microscope and low-temperature magnetic measurements. The MDA-MB-231 cancer cells were incubated with synthesized M-HFn nanoparticles for 24 hours in Dulbecco's Modified Eagle's Medium. In vitro MRI of cell pellets after M-HFn labeling was performed at 7 T. Iron uptake of cells was analyzed by Prussian blue staining and inductively coupled plasma mass spectrometry. Immunohistochemical staining by using the peroxidase-like activity of M-HFn nanoparticles was carried out on MDA-MB-231 tumor tissue paraffin sections.

Results: The saturation magnetization (M(s)), relaxivity, and peroxidase-like activity of synthesized M-HFn nanoparticles were monotonously increased with the size of ferrimagnetic cores. The M-HFn nanoparticles with the largest core size of 5.3 nm exhibit the strongest saturation magnetization, the highest peroxidase activity in immunohistochemical staining, and the highest r2 of 321 mM(-1) s(-1), allowing to detect MDA-MB-231 breast cancer cells as low as 10(4) cells mL(-1).

Conclusion: The magnetic properties, relaxivity, and peroxidase-like activity of M-HFn nanoparticles are size dependent, which indicates that M-HFn nanoparticles with larger magnetite core can significantly enhance performance in MRI and staining of cancer cells.

No MeSH data available.


Related in: MedlinePlus