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Enhanced magnetic resonance imaging and staining of cancer cells using ferrimagnetic H-ferritin nanoparticles with increasing core size.

Cai Y, Cao C, He X, Yang C, Tian L, Zhu R, Pan Y - Int J Nanomedicine (2015)

Bottom Line: In vitro MRI of cell pellets after M-HFn labeling was performed at 7 T.Iron uptake of cells was analyzed by Prussian blue staining and inductively coupled plasma mass spectrometry.The saturation magnetization (M(s)), relaxivity, and peroxidase-like activity of synthesized M-HFn nanoparticles were monotonously increased with the size of ferrimagnetic cores.

View Article: PubMed Central - PubMed

Affiliation: France-China Bio-Mineralization and Nano-Structures Laboratory, Key Laboratory of the Earth and Planetary Physics, Institute of Geology and Geophysics, Chinese Academy of Sciences, People's Republic of China ; Paleomagnetism and Geochronology Laboratory, Key Laboratory of the Earth and Planetary Physics, Institute of Geology and Geophysics, Chinese Academy of Sciences, People's Republic of China ; University of Chinese Academy of Sciences, Beijing, People's Republic of China.

ABSTRACT

Purpose: This study is to demonstrate the nanoscale size effect of ferrimagnetic H-ferritin (M-HFn) nanoparticles on magnetic properties, relaxivity, enzyme mimetic activities, and application in magnetic resonance imaging (MRI) and immunohistochemical staining of cancer cells.

Materials and methods: M-HFn nanoparticles with different sizes of magnetite cores in the range of 2.7-5.3 nm were synthesized through loading different amounts of iron into recombinant human H chain ferritin (HFn) shells. Core size, crystallinity, and magnetic properties of those M-HFn nanoparticles were analyzed by transmission electron microscope and low-temperature magnetic measurements. The MDA-MB-231 cancer cells were incubated with synthesized M-HFn nanoparticles for 24 hours in Dulbecco's Modified Eagle's Medium. In vitro MRI of cell pellets after M-HFn labeling was performed at 7 T. Iron uptake of cells was analyzed by Prussian blue staining and inductively coupled plasma mass spectrometry. Immunohistochemical staining by using the peroxidase-like activity of M-HFn nanoparticles was carried out on MDA-MB-231 tumor tissue paraffin sections.

Results: The saturation magnetization (M(s)), relaxivity, and peroxidase-like activity of synthesized M-HFn nanoparticles were monotonously increased with the size of ferrimagnetic cores. The M-HFn nanoparticles with the largest core size of 5.3 nm exhibit the strongest saturation magnetization, the highest peroxidase activity in immunohistochemical staining, and the highest r2 of 321 mM(-1) s(-1), allowing to detect MDA-MB-231 breast cancer cells as low as 10(4) cells mL(-1).

Conclusion: The magnetic properties, relaxivity, and peroxidase-like activity of M-HFn nanoparticles are size dependent, which indicates that M-HFn nanoparticles with larger magnetite core can significantly enhance performance in MRI and staining of cancer cells.

No MeSH data available.


Related in: MedlinePlus

TEM analysis of M-HFn nanoparticles.Notes: (A) TEM graphs of M-HFn1000, M-HFn3000, M-HFn5000, and M-HFn7000. Scale bar is 10 nm. (B) The high-resolution TEM images of these four M-HFn samples. Scale bar is 1 nm. (C) Corresponding SAED patterns of M-HFn nanoparticles. (D) Size histograms of M-HFn nanoparticles.Abbreviations: M-HFn, ferrimagnetic H-ferritin; TEM, transmission electron microscope; SAED, selected area electron diffraction.
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f1-ijn-10-2619: TEM analysis of M-HFn nanoparticles.Notes: (A) TEM graphs of M-HFn1000, M-HFn3000, M-HFn5000, and M-HFn7000. Scale bar is 10 nm. (B) The high-resolution TEM images of these four M-HFn samples. Scale bar is 1 nm. (C) Corresponding SAED patterns of M-HFn nanoparticles. (D) Size histograms of M-HFn nanoparticles.Abbreviations: M-HFn, ferrimagnetic H-ferritin; TEM, transmission electron microscope; SAED, selected area electron diffraction.

Mentions: By loading 1,000, 3,000, 5,000, and 7,000 iron atoms, the synthesized M-HFn nanoparticle samples M-HFn1000, M-HFn3000, M-HFn5000, and M-HFn7000, respectively, in the present study are monodispersed (Figure 1A), having well-crystalline cores with clear lattice fringes (Figure 1B). No obvious lattice defects (such as dislocation and stacking fault) were found. The SAED patterns consist of five distinct rings (Figure 1C), indexed to be the (220), (311), (400), (511), and (440) lattice planes of magnetite. The mean size of the magnetite cores in the M-HFn1000, M-HFn3000, M-HFn5000, and M-HFn7000 nanoparticles is 2.7±0.6 nm, 3.3±0.8 nm, 4.4±1.2 nm, and 5.3±1.3 nm, respectively (Figure 1D and Table 1), with major_axis/minor_axis ratios of 1.15, 1.13, 1.12, and 1.11, respectively.


Enhanced magnetic resonance imaging and staining of cancer cells using ferrimagnetic H-ferritin nanoparticles with increasing core size.

Cai Y, Cao C, He X, Yang C, Tian L, Zhu R, Pan Y - Int J Nanomedicine (2015)

TEM analysis of M-HFn nanoparticles.Notes: (A) TEM graphs of M-HFn1000, M-HFn3000, M-HFn5000, and M-HFn7000. Scale bar is 10 nm. (B) The high-resolution TEM images of these four M-HFn samples. Scale bar is 1 nm. (C) Corresponding SAED patterns of M-HFn nanoparticles. (D) Size histograms of M-HFn nanoparticles.Abbreviations: M-HFn, ferrimagnetic H-ferritin; TEM, transmission electron microscope; SAED, selected area electron diffraction.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4388082&req=5

f1-ijn-10-2619: TEM analysis of M-HFn nanoparticles.Notes: (A) TEM graphs of M-HFn1000, M-HFn3000, M-HFn5000, and M-HFn7000. Scale bar is 10 nm. (B) The high-resolution TEM images of these four M-HFn samples. Scale bar is 1 nm. (C) Corresponding SAED patterns of M-HFn nanoparticles. (D) Size histograms of M-HFn nanoparticles.Abbreviations: M-HFn, ferrimagnetic H-ferritin; TEM, transmission electron microscope; SAED, selected area electron diffraction.
Mentions: By loading 1,000, 3,000, 5,000, and 7,000 iron atoms, the synthesized M-HFn nanoparticle samples M-HFn1000, M-HFn3000, M-HFn5000, and M-HFn7000, respectively, in the present study are monodispersed (Figure 1A), having well-crystalline cores with clear lattice fringes (Figure 1B). No obvious lattice defects (such as dislocation and stacking fault) were found. The SAED patterns consist of five distinct rings (Figure 1C), indexed to be the (220), (311), (400), (511), and (440) lattice planes of magnetite. The mean size of the magnetite cores in the M-HFn1000, M-HFn3000, M-HFn5000, and M-HFn7000 nanoparticles is 2.7±0.6 nm, 3.3±0.8 nm, 4.4±1.2 nm, and 5.3±1.3 nm, respectively (Figure 1D and Table 1), with major_axis/minor_axis ratios of 1.15, 1.13, 1.12, and 1.11, respectively.

Bottom Line: In vitro MRI of cell pellets after M-HFn labeling was performed at 7 T.Iron uptake of cells was analyzed by Prussian blue staining and inductively coupled plasma mass spectrometry.The saturation magnetization (M(s)), relaxivity, and peroxidase-like activity of synthesized M-HFn nanoparticles were monotonously increased with the size of ferrimagnetic cores.

View Article: PubMed Central - PubMed

Affiliation: France-China Bio-Mineralization and Nano-Structures Laboratory, Key Laboratory of the Earth and Planetary Physics, Institute of Geology and Geophysics, Chinese Academy of Sciences, People's Republic of China ; Paleomagnetism and Geochronology Laboratory, Key Laboratory of the Earth and Planetary Physics, Institute of Geology and Geophysics, Chinese Academy of Sciences, People's Republic of China ; University of Chinese Academy of Sciences, Beijing, People's Republic of China.

ABSTRACT

Purpose: This study is to demonstrate the nanoscale size effect of ferrimagnetic H-ferritin (M-HFn) nanoparticles on magnetic properties, relaxivity, enzyme mimetic activities, and application in magnetic resonance imaging (MRI) and immunohistochemical staining of cancer cells.

Materials and methods: M-HFn nanoparticles with different sizes of magnetite cores in the range of 2.7-5.3 nm were synthesized through loading different amounts of iron into recombinant human H chain ferritin (HFn) shells. Core size, crystallinity, and magnetic properties of those M-HFn nanoparticles were analyzed by transmission electron microscope and low-temperature magnetic measurements. The MDA-MB-231 cancer cells were incubated with synthesized M-HFn nanoparticles for 24 hours in Dulbecco's Modified Eagle's Medium. In vitro MRI of cell pellets after M-HFn labeling was performed at 7 T. Iron uptake of cells was analyzed by Prussian blue staining and inductively coupled plasma mass spectrometry. Immunohistochemical staining by using the peroxidase-like activity of M-HFn nanoparticles was carried out on MDA-MB-231 tumor tissue paraffin sections.

Results: The saturation magnetization (M(s)), relaxivity, and peroxidase-like activity of synthesized M-HFn nanoparticles were monotonously increased with the size of ferrimagnetic cores. The M-HFn nanoparticles with the largest core size of 5.3 nm exhibit the strongest saturation magnetization, the highest peroxidase activity in immunohistochemical staining, and the highest r2 of 321 mM(-1) s(-1), allowing to detect MDA-MB-231 breast cancer cells as low as 10(4) cells mL(-1).

Conclusion: The magnetic properties, relaxivity, and peroxidase-like activity of M-HFn nanoparticles are size dependent, which indicates that M-HFn nanoparticles with larger magnetite core can significantly enhance performance in MRI and staining of cancer cells.

No MeSH data available.


Related in: MedlinePlus