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Synthesis and anti-fungal effect of silver nanoparticles-chitosan composite particles.

Wang LS, Wang CY, Yang CH, Hsieh CL, Chen SY, Shen CY, Wang JJ, Huang KS - Int J Nanomedicine (2015)

Bottom Line: The diameter of the synthesized chitosan composite particles ranged from 1.7 mm to 2.5 mm, and the embedded silver nanoparticles were measured to be 15 ± 3.3 nm.The results show that the silver nanoparticles were distributed over the surface and interior of the chitosan spheres.The fabricated spheres had macroporous property, and could be used for many applications such as fungicidal agents in the future.

View Article: PubMed Central - PubMed

Affiliation: Department of Electrical Engineering, I-Shou University, Kaohsiung, Taiwan ; Department of Biomedical Engineering, I-Shou University, Kaohsiung, Taiwan ; The School of Chinese Medicine for Post-Baccalaureate, I-Shou University, Kaohsiung, Taiwan ; Department of Chinese Medicine, E-Da Hospital, Kaohsiung, Taiwan.

ABSTRACT
Silver nanoparticles have been used in various fields, and several synthesis processes have been developed. The stability and dispersion of the synthesized nanoparticles is vital. The present article describes a novel approach for one-step synthesis of silver nanoparticles-embedded chitosan particles. The proposed approach was applied to simultaneously obtain and stabilize silver nanoparticles in a chitosan polymer matrix in-situ. The diameter of the synthesized chitosan composite particles ranged from 1.7 mm to 2.5 mm, and the embedded silver nanoparticles were measured to be 15 ± 3.3 nm. Further, the analyses of ultraviolet-visible spectroscopy, energy dispersive spectroscopy, and X-ray diffraction were employed to characterize the prepared composites. The results show that the silver nanoparticles were distributed over the surface and interior of the chitosan spheres. The fabricated spheres had macroporous property, and could be used for many applications such as fungicidal agents in the future.

No MeSH data available.


Ag@chitosan anti-fungal effect.Notes: (A) Flow chart of silver nanoparticles’ anti-fungal effect. (B) and (C) the anti-fungal effect of chitosan microspheres and Ag@chitosan. *P<0.05, **P<0.01.Abbreviations: Ag@chitosan, silver nanoparticles–chitosan composite spheres; Cm, C. militaris; Ac, A. cinnamomea; BCRC, Biore source Collection and Research Center.
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f6-ijn-10-2685: Ag@chitosan anti-fungal effect.Notes: (A) Flow chart of silver nanoparticles’ anti-fungal effect. (B) and (C) the anti-fungal effect of chitosan microspheres and Ag@chitosan. *P<0.05, **P<0.01.Abbreviations: Ag@chitosan, silver nanoparticles–chitosan composite spheres; Cm, C. militaris; Ac, A. cinnamomea; BCRC, Biore source Collection and Research Center.

Mentions: Figure 6 shows the anti-fungal effect. We found that Cm is inhibited by dissolved chitosan and silver nanoparticles–chitosan composite spheres (Ag@chitosan) on Day 9 (the inhibition ring diameter of chitosan is 3.2±0.1 cm and Ag@ chitosan is 3.533±0.217 cm) and Day 18 (the inhibition ring diameter of chitosan is 2.7±0.2 cm and Ag@chitosan is 3.2±0.058 cm) but not Ac. This finding indicates that Ag@ chitosan and chitosan can both inhibit the growth of Cm but the activity of Ag@chitosan is better than chitosan (on Day 9, P<0.05; on Day 18, P<0.01). It is interesting to note that no inhibition ring is formed in Ac group, indicating that Ac is not affected by Ag@chitosan and chitosan. It is worth mentioning that on Day 9 we found not only chitosan but also Ag@chitosan show the best anti-fungal effects. We speculate that the reduction of anti-fungal effect might be caused by the degradation of chitosan and Ag@chitosan. As widely known, both chitosan and Ag@chitosan can inhibit many kinds of microorganisms. In this study, we found that Ag@chitosan or chitosan can inhibit the growth of Cm but not Ac. Taken all together, we thought chitosan and Ag@chistosan can act as a potent reagent to enhance Ac production by inhibiting other microorganism growth.


Synthesis and anti-fungal effect of silver nanoparticles-chitosan composite particles.

Wang LS, Wang CY, Yang CH, Hsieh CL, Chen SY, Shen CY, Wang JJ, Huang KS - Int J Nanomedicine (2015)

Ag@chitosan anti-fungal effect.Notes: (A) Flow chart of silver nanoparticles’ anti-fungal effect. (B) and (C) the anti-fungal effect of chitosan microspheres and Ag@chitosan. *P<0.05, **P<0.01.Abbreviations: Ag@chitosan, silver nanoparticles–chitosan composite spheres; Cm, C. militaris; Ac, A. cinnamomea; BCRC, Biore source Collection and Research Center.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4388074&req=5

f6-ijn-10-2685: Ag@chitosan anti-fungal effect.Notes: (A) Flow chart of silver nanoparticles’ anti-fungal effect. (B) and (C) the anti-fungal effect of chitosan microspheres and Ag@chitosan. *P<0.05, **P<0.01.Abbreviations: Ag@chitosan, silver nanoparticles–chitosan composite spheres; Cm, C. militaris; Ac, A. cinnamomea; BCRC, Biore source Collection and Research Center.
Mentions: Figure 6 shows the anti-fungal effect. We found that Cm is inhibited by dissolved chitosan and silver nanoparticles–chitosan composite spheres (Ag@chitosan) on Day 9 (the inhibition ring diameter of chitosan is 3.2±0.1 cm and Ag@ chitosan is 3.533±0.217 cm) and Day 18 (the inhibition ring diameter of chitosan is 2.7±0.2 cm and Ag@chitosan is 3.2±0.058 cm) but not Ac. This finding indicates that Ag@ chitosan and chitosan can both inhibit the growth of Cm but the activity of Ag@chitosan is better than chitosan (on Day 9, P<0.05; on Day 18, P<0.01). It is interesting to note that no inhibition ring is formed in Ac group, indicating that Ac is not affected by Ag@chitosan and chitosan. It is worth mentioning that on Day 9 we found not only chitosan but also Ag@chitosan show the best anti-fungal effects. We speculate that the reduction of anti-fungal effect might be caused by the degradation of chitosan and Ag@chitosan. As widely known, both chitosan and Ag@chitosan can inhibit many kinds of microorganisms. In this study, we found that Ag@chitosan or chitosan can inhibit the growth of Cm but not Ac. Taken all together, we thought chitosan and Ag@chistosan can act as a potent reagent to enhance Ac production by inhibiting other microorganism growth.

Bottom Line: The diameter of the synthesized chitosan composite particles ranged from 1.7 mm to 2.5 mm, and the embedded silver nanoparticles were measured to be 15 ± 3.3 nm.The results show that the silver nanoparticles were distributed over the surface and interior of the chitosan spheres.The fabricated spheres had macroporous property, and could be used for many applications such as fungicidal agents in the future.

View Article: PubMed Central - PubMed

Affiliation: Department of Electrical Engineering, I-Shou University, Kaohsiung, Taiwan ; Department of Biomedical Engineering, I-Shou University, Kaohsiung, Taiwan ; The School of Chinese Medicine for Post-Baccalaureate, I-Shou University, Kaohsiung, Taiwan ; Department of Chinese Medicine, E-Da Hospital, Kaohsiung, Taiwan.

ABSTRACT
Silver nanoparticles have been used in various fields, and several synthesis processes have been developed. The stability and dispersion of the synthesized nanoparticles is vital. The present article describes a novel approach for one-step synthesis of silver nanoparticles-embedded chitosan particles. The proposed approach was applied to simultaneously obtain and stabilize silver nanoparticles in a chitosan polymer matrix in-situ. The diameter of the synthesized chitosan composite particles ranged from 1.7 mm to 2.5 mm, and the embedded silver nanoparticles were measured to be 15 ± 3.3 nm. Further, the analyses of ultraviolet-visible spectroscopy, energy dispersive spectroscopy, and X-ray diffraction were employed to characterize the prepared composites. The results show that the silver nanoparticles were distributed over the surface and interior of the chitosan spheres. The fabricated spheres had macroporous property, and could be used for many applications such as fungicidal agents in the future.

No MeSH data available.