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Application of silicalite-modified electrode for the development of sucrose biosensor with improved characteristics.

Pyeshkova VM, Dudchenko OY, Soldatkin OO, Kasap BO, Lagarde F, Kurç BA, Dzyadevych SV - Nanoscale Res Lett (2015)

Bottom Line: Polyethylenimine/glutaraldehyde/silicalite-modified biosensors showed higher sensitivity compared with others type of biosensors.The obtained results had good correlation with results obtained by HPLC.Thus, polyethylenimine/glutaraldehyde/silicalite-modified biosensors have shown perspective characteristics for the development of effective conductometric enzyme biosensors.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Biomolecular Electronics, Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, 150 Zabolotnogo Str., 03680 Kyiv, Ukraine ; Institute of High Technologies, Taras Shevchenko National University of Kyiv, 64 Volodymyrska Street, 01601 Kyiv, Ukraine.

ABSTRACT
The application of silicalite for improvement of working characteristics of conductometric enzyme biosensors for determination of sucrose was studied in this research. Biosensors based on different types of silicalite-modified electrodes were studied and compared according to their analytical characteristics. Polyethylenimine/glutaraldehyde/silicalite-modified biosensors showed higher sensitivity compared with others type of biosensors. Moreover, the polyethylenimine/glutaraldehyde/silicalite sucrose biosensors were characterized by high selectivity and signal reproducibility (relative standard deviation (RSD) = 2.78% for glucose measurements and RSD = 3.2% for sucrose measurements). Proposed biosensors were used for determination of sucrose in different samples of beverages. The obtained results had good correlation with results obtained by HPLC. Thus, polyethylenimine/glutaraldehyde/silicalite-modified biosensors have shown perspective characteristics for the development of effective conductometric enzyme biosensors.

No MeSH data available.


Calibration curves of PEI/GA/Sil sucrose biosensors. Responses are shown for adding of glucose (1) and sucrose (2) respectively.
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Fig7: Calibration curves of PEI/GA/Sil sucrose biosensors. Responses are shown for adding of glucose (1) and sucrose (2) respectively.

Mentions: The calibration curves to glucose and sucrose by PEI/GA/Sil sucrose biosensor are shown on Figure 7. The PEI/GA/Sil sucrose biosensors showed extended linear range of sucrose detection comparing with traditional type of biosensors based on immobilization in GA without silicalite. The linear range of PEI/GA/Sil sucrose biosensors was 0.0035 to 4 mM for sucrose and 0.0015 to 1.75 mM for glucose (Figure 7). The limit of detection (LOD) for glucose determination was 1.5 μM and LOD for sucrose determination was 3.5 μM.Figure 7


Application of silicalite-modified electrode for the development of sucrose biosensor with improved characteristics.

Pyeshkova VM, Dudchenko OY, Soldatkin OO, Kasap BO, Lagarde F, Kurç BA, Dzyadevych SV - Nanoscale Res Lett (2015)

Calibration curves of PEI/GA/Sil sucrose biosensors. Responses are shown for adding of glucose (1) and sucrose (2) respectively.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4388068&req=5

Fig7: Calibration curves of PEI/GA/Sil sucrose biosensors. Responses are shown for adding of glucose (1) and sucrose (2) respectively.
Mentions: The calibration curves to glucose and sucrose by PEI/GA/Sil sucrose biosensor are shown on Figure 7. The PEI/GA/Sil sucrose biosensors showed extended linear range of sucrose detection comparing with traditional type of biosensors based on immobilization in GA without silicalite. The linear range of PEI/GA/Sil sucrose biosensors was 0.0035 to 4 mM for sucrose and 0.0015 to 1.75 mM for glucose (Figure 7). The limit of detection (LOD) for glucose determination was 1.5 μM and LOD for sucrose determination was 3.5 μM.Figure 7

Bottom Line: Polyethylenimine/glutaraldehyde/silicalite-modified biosensors showed higher sensitivity compared with others type of biosensors.The obtained results had good correlation with results obtained by HPLC.Thus, polyethylenimine/glutaraldehyde/silicalite-modified biosensors have shown perspective characteristics for the development of effective conductometric enzyme biosensors.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Biomolecular Electronics, Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, 150 Zabolotnogo Str., 03680 Kyiv, Ukraine ; Institute of High Technologies, Taras Shevchenko National University of Kyiv, 64 Volodymyrska Street, 01601 Kyiv, Ukraine.

ABSTRACT
The application of silicalite for improvement of working characteristics of conductometric enzyme biosensors for determination of sucrose was studied in this research. Biosensors based on different types of silicalite-modified electrodes were studied and compared according to their analytical characteristics. Polyethylenimine/glutaraldehyde/silicalite-modified biosensors showed higher sensitivity compared with others type of biosensors. Moreover, the polyethylenimine/glutaraldehyde/silicalite sucrose biosensors were characterized by high selectivity and signal reproducibility (relative standard deviation (RSD) = 2.78% for glucose measurements and RSD = 3.2% for sucrose measurements). Proposed biosensors were used for determination of sucrose in different samples of beverages. The obtained results had good correlation with results obtained by HPLC. Thus, polyethylenimine/glutaraldehyde/silicalite-modified biosensors have shown perspective characteristics for the development of effective conductometric enzyme biosensors.

No MeSH data available.