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Application of silicalite-modified electrode for the development of sucrose biosensor with improved characteristics.

Pyeshkova VM, Dudchenko OY, Soldatkin OO, Kasap BO, Lagarde F, Kurç BA, Dzyadevych SV - Nanoscale Res Lett (2015)

Bottom Line: Polyethylenimine/glutaraldehyde/silicalite-modified biosensors showed higher sensitivity compared with others type of biosensors.The obtained results had good correlation with results obtained by HPLC.Thus, polyethylenimine/glutaraldehyde/silicalite-modified biosensors have shown perspective characteristics for the development of effective conductometric enzyme biosensors.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Biomolecular Electronics, Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, 150 Zabolotnogo Str., 03680 Kyiv, Ukraine ; Institute of High Technologies, Taras Shevchenko National University of Kyiv, 64 Volodymyrska Street, 01601 Kyiv, Ukraine.

ABSTRACT
The application of silicalite for improvement of working characteristics of conductometric enzyme biosensors for determination of sucrose was studied in this research. Biosensors based on different types of silicalite-modified electrodes were studied and compared according to their analytical characteristics. Polyethylenimine/glutaraldehyde/silicalite-modified biosensors showed higher sensitivity compared with others type of biosensors. Moreover, the polyethylenimine/glutaraldehyde/silicalite sucrose biosensors were characterized by high selectivity and signal reproducibility (relative standard deviation (RSD) = 2.78% for glucose measurements and RSD = 3.2% for sucrose measurements). Proposed biosensors were used for determination of sucrose in different samples of beverages. The obtained results had good correlation with results obtained by HPLC. Thus, polyethylenimine/glutaraldehyde/silicalite-modified biosensors have shown perspective characteristics for the development of effective conductometric enzyme biosensors.

No MeSH data available.


Signal reproducibility of PEI/GA/Sil sucrose biosensor. Responses of sucrose biosensor: (1) responses to 0.25 mM glucose, (2) responses to 0.25 mM sucrose.
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Fig6: Signal reproducibility of PEI/GA/Sil sucrose biosensor. Responses of sucrose biosensor: (1) responses to 0.25 mM glucose, (2) responses to 0.25 mM sucrose.

Mentions: Reproducibility is one of the most important working characteristics of biosensors. To determine signal reproducibility, the biosensors’ responses to 0.25 mM glucose and sucrose were measured during one working day with 10- to 15-min intervals. The biosensors were kept in the continuously stirred buffer solution at room temperature during intervals between measurements. As seen from Figure 6, the biosensor responses were highly reproducible. The relative standard deviation (RSD) for glucose measurements was 2.78% and for sucrose measurements was 3.2% which is quite acceptable value (Figure 6).Figure 6


Application of silicalite-modified electrode for the development of sucrose biosensor with improved characteristics.

Pyeshkova VM, Dudchenko OY, Soldatkin OO, Kasap BO, Lagarde F, Kurç BA, Dzyadevych SV - Nanoscale Res Lett (2015)

Signal reproducibility of PEI/GA/Sil sucrose biosensor. Responses of sucrose biosensor: (1) responses to 0.25 mM glucose, (2) responses to 0.25 mM sucrose.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4388068&req=5

Fig6: Signal reproducibility of PEI/GA/Sil sucrose biosensor. Responses of sucrose biosensor: (1) responses to 0.25 mM glucose, (2) responses to 0.25 mM sucrose.
Mentions: Reproducibility is one of the most important working characteristics of biosensors. To determine signal reproducibility, the biosensors’ responses to 0.25 mM glucose and sucrose were measured during one working day with 10- to 15-min intervals. The biosensors were kept in the continuously stirred buffer solution at room temperature during intervals between measurements. As seen from Figure 6, the biosensor responses were highly reproducible. The relative standard deviation (RSD) for glucose measurements was 2.78% and for sucrose measurements was 3.2% which is quite acceptable value (Figure 6).Figure 6

Bottom Line: Polyethylenimine/glutaraldehyde/silicalite-modified biosensors showed higher sensitivity compared with others type of biosensors.The obtained results had good correlation with results obtained by HPLC.Thus, polyethylenimine/glutaraldehyde/silicalite-modified biosensors have shown perspective characteristics for the development of effective conductometric enzyme biosensors.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Biomolecular Electronics, Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, 150 Zabolotnogo Str., 03680 Kyiv, Ukraine ; Institute of High Technologies, Taras Shevchenko National University of Kyiv, 64 Volodymyrska Street, 01601 Kyiv, Ukraine.

ABSTRACT
The application of silicalite for improvement of working characteristics of conductometric enzyme biosensors for determination of sucrose was studied in this research. Biosensors based on different types of silicalite-modified electrodes were studied and compared according to their analytical characteristics. Polyethylenimine/glutaraldehyde/silicalite-modified biosensors showed higher sensitivity compared with others type of biosensors. Moreover, the polyethylenimine/glutaraldehyde/silicalite sucrose biosensors were characterized by high selectivity and signal reproducibility (relative standard deviation (RSD) = 2.78% for glucose measurements and RSD = 3.2% for sucrose measurements). Proposed biosensors were used for determination of sucrose in different samples of beverages. The obtained results had good correlation with results obtained by HPLC. Thus, polyethylenimine/glutaraldehyde/silicalite-modified biosensors have shown perspective characteristics for the development of effective conductometric enzyme biosensors.

No MeSH data available.