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Application of silicalite-modified electrode for the development of sucrose biosensor with improved characteristics.

Pyeshkova VM, Dudchenko OY, Soldatkin OO, Kasap BO, Lagarde F, Kurç BA, Dzyadevych SV - Nanoscale Res Lett (2015)

Bottom Line: Polyethylenimine/glutaraldehyde/silicalite-modified biosensors showed higher sensitivity compared with others type of biosensors.The obtained results had good correlation with results obtained by HPLC.Thus, polyethylenimine/glutaraldehyde/silicalite-modified biosensors have shown perspective characteristics for the development of effective conductometric enzyme biosensors.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Biomolecular Electronics, Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, 150 Zabolotnogo Str., 03680 Kyiv, Ukraine ; Institute of High Technologies, Taras Shevchenko National University of Kyiv, 64 Volodymyrska Street, 01601 Kyiv, Ukraine.

ABSTRACT
The application of silicalite for improvement of working characteristics of conductometric enzyme biosensors for determination of sucrose was studied in this research. Biosensors based on different types of silicalite-modified electrodes were studied and compared according to their analytical characteristics. Polyethylenimine/glutaraldehyde/silicalite-modified biosensors showed higher sensitivity compared with others type of biosensors. Moreover, the polyethylenimine/glutaraldehyde/silicalite sucrose biosensors were characterized by high selectivity and signal reproducibility (relative standard deviation (RSD) = 2.78% for glucose measurements and RSD = 3.2% for sucrose measurements). Proposed biosensors were used for determination of sucrose in different samples of beverages. The obtained results had good correlation with results obtained by HPLC. Thus, polyethylenimine/glutaraldehyde/silicalite-modified biosensors have shown perspective characteristics for the development of effective conductometric enzyme biosensors.

No MeSH data available.


Selectivity test of PEI/GA/Sil sucrose biosensor. Measurements were carried out in 5 mM phosphate buffer solution.
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Fig5: Selectivity test of PEI/GA/Sil sucrose biosensor. Measurements were carried out in 5 mM phosphate buffer solution.

Mentions: First of all, we studied the selectivity of PEI/GA/Sil sucrose biosensors. Biosensor selectivity, one of important analytical characteristics, depends on choice of both biological recognition element and transducer. An influence of interfering components (some carbohydrates) on response value was studied to test biosensor selectivity. For that purpose, glucose, maltose, sucrose, lactose, fructose, arabinose and galactose in concentration of 0.5 mM were added to a working cell (Figure 5).Figure 5


Application of silicalite-modified electrode for the development of sucrose biosensor with improved characteristics.

Pyeshkova VM, Dudchenko OY, Soldatkin OO, Kasap BO, Lagarde F, Kurç BA, Dzyadevych SV - Nanoscale Res Lett (2015)

Selectivity test of PEI/GA/Sil sucrose biosensor. Measurements were carried out in 5 mM phosphate buffer solution.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4388068&req=5

Fig5: Selectivity test of PEI/GA/Sil sucrose biosensor. Measurements were carried out in 5 mM phosphate buffer solution.
Mentions: First of all, we studied the selectivity of PEI/GA/Sil sucrose biosensors. Biosensor selectivity, one of important analytical characteristics, depends on choice of both biological recognition element and transducer. An influence of interfering components (some carbohydrates) on response value was studied to test biosensor selectivity. For that purpose, glucose, maltose, sucrose, lactose, fructose, arabinose and galactose in concentration of 0.5 mM were added to a working cell (Figure 5).Figure 5

Bottom Line: Polyethylenimine/glutaraldehyde/silicalite-modified biosensors showed higher sensitivity compared with others type of biosensors.The obtained results had good correlation with results obtained by HPLC.Thus, polyethylenimine/glutaraldehyde/silicalite-modified biosensors have shown perspective characteristics for the development of effective conductometric enzyme biosensors.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Biomolecular Electronics, Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, 150 Zabolotnogo Str., 03680 Kyiv, Ukraine ; Institute of High Technologies, Taras Shevchenko National University of Kyiv, 64 Volodymyrska Street, 01601 Kyiv, Ukraine.

ABSTRACT
The application of silicalite for improvement of working characteristics of conductometric enzyme biosensors for determination of sucrose was studied in this research. Biosensors based on different types of silicalite-modified electrodes were studied and compared according to their analytical characteristics. Polyethylenimine/glutaraldehyde/silicalite-modified biosensors showed higher sensitivity compared with others type of biosensors. Moreover, the polyethylenimine/glutaraldehyde/silicalite sucrose biosensors were characterized by high selectivity and signal reproducibility (relative standard deviation (RSD) = 2.78% for glucose measurements and RSD = 3.2% for sucrose measurements). Proposed biosensors were used for determination of sucrose in different samples of beverages. The obtained results had good correlation with results obtained by HPLC. Thus, polyethylenimine/glutaraldehyde/silicalite-modified biosensors have shown perspective characteristics for the development of effective conductometric enzyme biosensors.

No MeSH data available.