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Influence of cardiorespiratory fitness on PPARG mRNA expression using monozygotic twin case control.

Queiroga MR, Barbieri RA, Ferreira SA, Luchessi AD, Hirata RD, Hirata MH, Kokubun E - J Diabetes Res (2015)

Bottom Line: Twins from the discordant group had differences in VO2max values (D-high = 45.9 ± 10.0 versus D-low = 32.4 ± 10.6 mL·kg(-1)·min(-1), P = 0.025), while no differences were found in the concordant group (C-high = 42.4 ± 9.2 versus C-low = 38.8 ± 9.8 mL·kg(-1)·min(-1), P = 0.952).Twins D-high had 1.78-fold greater PPARG expression when compared with twins D-low (P = 0.048).In conclusion, the cardiorespiratory fitness may modulate PPARG expression in childhood and adolescence, independently of the genetic background.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Educação Física da Universidade Estadual do Centro-Oeste (UNICENTRO), Rua Simeão Camargo Varela de Sá, 03, 85040-080 Guarapuava, PR, Brazil.

ABSTRACT
The influence of cardiorespiratory fitness (VO2max) on anthropometric variables and PPARG mRNA expression was investigated. Monozygotic twin pairs aged 11-18 years were grouped into discordant (D) and concordant (C) high and low VO2max groups. VO2max was determined by progressive maximal exercise test on treadmill with gas exchange analysis. Body mass (BM), BMI, waist circumference (WC), triceps (TR), and subscapular (SB) skinfold thicknesses were measured. Twins from the discordant group had differences in VO2max values (D-high = 45.9 ± 10.0 versus D-low = 32.4 ± 10.6 mL·kg(-1)·min(-1), P = 0.025), while no differences were found in the concordant group (C-high = 42.4 ± 9.2 versus C-low = 38.8 ± 9.8 mL·kg(-1)·min(-1), P = 0.952). In discordant group, VO2max was negatively correlated with TR + SB (r = -0.540, P = 0.021) and positively correlated with PPARG expression in leukocytes (r = 0.952, P = 0.001). Moreover, PPARG expression was directly correlated with BM (r = 0.714, P = 0.047) and height (r = 0.762, P = 0.028). In concordant twins, VO2max was inversely correlated with BM (r = -0.290, P = 0.027), BMI (r = -0.472, P = 0.001), WC (r = -0.426, P = 0.001), and TR + SB (r = -0.739, P = 0.001). Twins D-high had 1.78-fold greater PPARG expression when compared with twins D-low (P = 0.048). In conclusion, the cardiorespiratory fitness may modulate PPARG expression in childhood and adolescence, independently of the genetic background.

Show MeSH
PPARG mRNA expression in blood total leukocytes from VO2max discordant and concordant MZ twins. mRNA expression is shown as ΔCt values in relation to GAPD (reference gene). Results between low and high VO2max within discordant (D-low versus D-high) and concordant (C-low with C-high) pairs were compared by paired Wilcoxon test.
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fig1: PPARG mRNA expression in blood total leukocytes from VO2max discordant and concordant MZ twins. mRNA expression is shown as ΔCt values in relation to GAPD (reference gene). Results between low and high VO2max within discordant (D-low versus D-high) and concordant (C-low with C-high) pairs were compared by paired Wilcoxon test.

Mentions: Regarding the PPARG mRNA expression, it was observed that discordant cotwins with the high VO2max values (D-High) had 1.78-fold greater PPARG mRNA expression when compared to cotwins with the low VO2max values (D-low) (P = 0.048) (Figure 1). In the concordant group, cotwins with low VO2max values (C-low) had 1.22-fold greater PPARG mRNA expression when compared to cotwins with high VO2max (C-high). However, there was no significant difference (P = 0.374) (Figure 1).


Influence of cardiorespiratory fitness on PPARG mRNA expression using monozygotic twin case control.

Queiroga MR, Barbieri RA, Ferreira SA, Luchessi AD, Hirata RD, Hirata MH, Kokubun E - J Diabetes Res (2015)

PPARG mRNA expression in blood total leukocytes from VO2max discordant and concordant MZ twins. mRNA expression is shown as ΔCt values in relation to GAPD (reference gene). Results between low and high VO2max within discordant (D-low versus D-high) and concordant (C-low with C-high) pairs were compared by paired Wilcoxon test.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4388019&req=5

fig1: PPARG mRNA expression in blood total leukocytes from VO2max discordant and concordant MZ twins. mRNA expression is shown as ΔCt values in relation to GAPD (reference gene). Results between low and high VO2max within discordant (D-low versus D-high) and concordant (C-low with C-high) pairs were compared by paired Wilcoxon test.
Mentions: Regarding the PPARG mRNA expression, it was observed that discordant cotwins with the high VO2max values (D-High) had 1.78-fold greater PPARG mRNA expression when compared to cotwins with the low VO2max values (D-low) (P = 0.048) (Figure 1). In the concordant group, cotwins with low VO2max values (C-low) had 1.22-fold greater PPARG mRNA expression when compared to cotwins with high VO2max (C-high). However, there was no significant difference (P = 0.374) (Figure 1).

Bottom Line: Twins from the discordant group had differences in VO2max values (D-high = 45.9 ± 10.0 versus D-low = 32.4 ± 10.6 mL·kg(-1)·min(-1), P = 0.025), while no differences were found in the concordant group (C-high = 42.4 ± 9.2 versus C-low = 38.8 ± 9.8 mL·kg(-1)·min(-1), P = 0.952).Twins D-high had 1.78-fold greater PPARG expression when compared with twins D-low (P = 0.048).In conclusion, the cardiorespiratory fitness may modulate PPARG expression in childhood and adolescence, independently of the genetic background.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Educação Física da Universidade Estadual do Centro-Oeste (UNICENTRO), Rua Simeão Camargo Varela de Sá, 03, 85040-080 Guarapuava, PR, Brazil.

ABSTRACT
The influence of cardiorespiratory fitness (VO2max) on anthropometric variables and PPARG mRNA expression was investigated. Monozygotic twin pairs aged 11-18 years were grouped into discordant (D) and concordant (C) high and low VO2max groups. VO2max was determined by progressive maximal exercise test on treadmill with gas exchange analysis. Body mass (BM), BMI, waist circumference (WC), triceps (TR), and subscapular (SB) skinfold thicknesses were measured. Twins from the discordant group had differences in VO2max values (D-high = 45.9 ± 10.0 versus D-low = 32.4 ± 10.6 mL·kg(-1)·min(-1), P = 0.025), while no differences were found in the concordant group (C-high = 42.4 ± 9.2 versus C-low = 38.8 ± 9.8 mL·kg(-1)·min(-1), P = 0.952). In discordant group, VO2max was negatively correlated with TR + SB (r = -0.540, P = 0.021) and positively correlated with PPARG expression in leukocytes (r = 0.952, P = 0.001). Moreover, PPARG expression was directly correlated with BM (r = 0.714, P = 0.047) and height (r = 0.762, P = 0.028). In concordant twins, VO2max was inversely correlated with BM (r = -0.290, P = 0.027), BMI (r = -0.472, P = 0.001), WC (r = -0.426, P = 0.001), and TR + SB (r = -0.739, P = 0.001). Twins D-high had 1.78-fold greater PPARG expression when compared with twins D-low (P = 0.048). In conclusion, the cardiorespiratory fitness may modulate PPARG expression in childhood and adolescence, independently of the genetic background.

Show MeSH