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Histopathological, ultrastructural, and immunohistochemical assessment of hippocampus structures of rats exposed to TCDD and high doses of tocopherol and acetylsalicylic acid.

Rosińczuk J, Dymarek R, Całkosiński I - Biomed Res Int (2015)

Bottom Line: The aim of the study was to determine the effect of dioxins on function, ultrastructure, and cytological and histological structure of hippocampus, particularly on expression of estrogen receptors in central nervous system as well as to define protective influence of tocopherol (TCP) and acetylsalicylic acid (ASA) on the decrease in activity of proinflammatory effects in central nervous system.Histopathological and ultrastructural image of hippocampus areas in rats, in which both TCP and ASA were used, is characterized by poorly expressed degenerative changes and smaller inflammatory reactivity.Using both TCP and ASA has a protective effect on functions of central nervous system.

View Article: PubMed Central - PubMed

Affiliation: Department of Nervous System Diseases, The Faculty of Health Science, Wroclaw Medical University, Bartla 5 Street, 51-618 Wrocław, Poland.

ABSTRACT
The effect of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) on central nervous system consists of changing expression of estrogen receptors, whereas the result of chronic inflammatory reaction caused by dioxin is occurrence of destructive changes in various organs connected with disturbed metabolism of connective tissue and damage of cells. The aim of the study was to determine the effect of dioxins on function, ultrastructure, and cytological and histological structure of hippocampus, particularly on expression of estrogen receptors in central nervous system as well as to define protective influence of tocopherol (TCP) and acetylsalicylic acid (ASA) on the decrease in activity of proinflammatory effects in central nervous system. It was shown that TCDD contributes to destructive and inflammatory changes along with demyelization of myelin sheaths and atrophy of estrogen receptors in hippocampus. Dioxin contributes to atrophy of estrogen receptors in hippocampus, in which also destructive and inflammatory changes were found along with demyelination of myelin sheaths. Histopathological and ultrastructural image of hippocampus areas in rats, in which both TCP and ASA were used, is characterized by poorly expressed degenerative changes and smaller inflammatory reactivity. Using both TCP and ASA has a protective effect on functions of central nervous system.

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Related in: MedlinePlus

Ultrastructural image of neurofibrils of hippocampus in group IPD (enlargement 18 000x). There is a clear degree of vacuolation of neuron cytoplasm (1); nerve fibers situated near nerve cell reveal the image of delamination of myelin sheath laminae (2).
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fig21: Ultrastructural image of neurofibrils of hippocampus in group IPD (enlargement 18 000x). There is a clear degree of vacuolation of neuron cytoplasm (1); nerve fibers situated near nerve cell reveal the image of delamination of myelin sheath laminae (2).

Mentions: In the group exposed to dioxin action with induced inflammation of IPD (Figure 21) occurrence of significant changes in ultrastructure of hippocampus. In perikaryon of nerve cells dilation of perinuclear space was stated. In the area of nucleus heterochromatin concentrates in the form of insulas or bands by nuclear membrane. Clearly exposed nucleolus with areas indicated that its disintegration was localized with vesicular ectasia of endoplasmic reticulum and clarifications of basic cytoplasm. Moreover mitochondria edema and dispersion of structures of Golgi apparatus were observed. Present myelinated fibers adjacent to the described perikaryon sometimes reveal vacuolation of axoplasm. In axoplasm there is also vacuolation of endoplasmic reticulum and mitochondria edema. In the process zone, segmentary delamination of myelin sheaths particularly myelinated fibers is found. In glia cells the following things are visible: vacuolation of cell cytoplasm and mitochondria edema, dispersion of reticulum within axoplasm and glia cells, and delamination of neurofilaments. Cells of glia tissue especially oligodendrocytes reveal degenerative changes of similar intensity like in nerve cells. In astroglia cells a smaller degree of damage can be found. The character of changes in oligodendrocytes takes form of vacuolation of endoplasmic reticulum and basal cytoplasm. General image of changes presents the state of cell edema. In blood capillary vessels there are delaminations between endothelium and adventitia, softening of endothelium cells, mitochondria edema, degenerative changes, and cell vacuolation. Moreover increased fibrogenesis can be observed and at times occurring connective tissue leads to clamping of capillary vessel lumen. In vessels there are numerous erythrocytes squeezing through their lumen and near vessel there are macrophages.


Histopathological, ultrastructural, and immunohistochemical assessment of hippocampus structures of rats exposed to TCDD and high doses of tocopherol and acetylsalicylic acid.

Rosińczuk J, Dymarek R, Całkosiński I - Biomed Res Int (2015)

Ultrastructural image of neurofibrils of hippocampus in group IPD (enlargement 18 000x). There is a clear degree of vacuolation of neuron cytoplasm (1); nerve fibers situated near nerve cell reveal the image of delamination of myelin sheath laminae (2).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4388018&req=5

fig21: Ultrastructural image of neurofibrils of hippocampus in group IPD (enlargement 18 000x). There is a clear degree of vacuolation of neuron cytoplasm (1); nerve fibers situated near nerve cell reveal the image of delamination of myelin sheath laminae (2).
Mentions: In the group exposed to dioxin action with induced inflammation of IPD (Figure 21) occurrence of significant changes in ultrastructure of hippocampus. In perikaryon of nerve cells dilation of perinuclear space was stated. In the area of nucleus heterochromatin concentrates in the form of insulas or bands by nuclear membrane. Clearly exposed nucleolus with areas indicated that its disintegration was localized with vesicular ectasia of endoplasmic reticulum and clarifications of basic cytoplasm. Moreover mitochondria edema and dispersion of structures of Golgi apparatus were observed. Present myelinated fibers adjacent to the described perikaryon sometimes reveal vacuolation of axoplasm. In axoplasm there is also vacuolation of endoplasmic reticulum and mitochondria edema. In the process zone, segmentary delamination of myelin sheaths particularly myelinated fibers is found. In glia cells the following things are visible: vacuolation of cell cytoplasm and mitochondria edema, dispersion of reticulum within axoplasm and glia cells, and delamination of neurofilaments. Cells of glia tissue especially oligodendrocytes reveal degenerative changes of similar intensity like in nerve cells. In astroglia cells a smaller degree of damage can be found. The character of changes in oligodendrocytes takes form of vacuolation of endoplasmic reticulum and basal cytoplasm. General image of changes presents the state of cell edema. In blood capillary vessels there are delaminations between endothelium and adventitia, softening of endothelium cells, mitochondria edema, degenerative changes, and cell vacuolation. Moreover increased fibrogenesis can be observed and at times occurring connective tissue leads to clamping of capillary vessel lumen. In vessels there are numerous erythrocytes squeezing through their lumen and near vessel there are macrophages.

Bottom Line: The aim of the study was to determine the effect of dioxins on function, ultrastructure, and cytological and histological structure of hippocampus, particularly on expression of estrogen receptors in central nervous system as well as to define protective influence of tocopherol (TCP) and acetylsalicylic acid (ASA) on the decrease in activity of proinflammatory effects in central nervous system.Histopathological and ultrastructural image of hippocampus areas in rats, in which both TCP and ASA were used, is characterized by poorly expressed degenerative changes and smaller inflammatory reactivity.Using both TCP and ASA has a protective effect on functions of central nervous system.

View Article: PubMed Central - PubMed

Affiliation: Department of Nervous System Diseases, The Faculty of Health Science, Wroclaw Medical University, Bartla 5 Street, 51-618 Wrocław, Poland.

ABSTRACT
The effect of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) on central nervous system consists of changing expression of estrogen receptors, whereas the result of chronic inflammatory reaction caused by dioxin is occurrence of destructive changes in various organs connected with disturbed metabolism of connective tissue and damage of cells. The aim of the study was to determine the effect of dioxins on function, ultrastructure, and cytological and histological structure of hippocampus, particularly on expression of estrogen receptors in central nervous system as well as to define protective influence of tocopherol (TCP) and acetylsalicylic acid (ASA) on the decrease in activity of proinflammatory effects in central nervous system. It was shown that TCDD contributes to destructive and inflammatory changes along with demyelization of myelin sheaths and atrophy of estrogen receptors in hippocampus. Dioxin contributes to atrophy of estrogen receptors in hippocampus, in which also destructive and inflammatory changes were found along with demyelination of myelin sheaths. Histopathological and ultrastructural image of hippocampus areas in rats, in which both TCP and ASA were used, is characterized by poorly expressed degenerative changes and smaller inflammatory reactivity. Using both TCP and ASA has a protective effect on functions of central nervous system.

Show MeSH
Related in: MedlinePlus