Limits...
Histopathological, ultrastructural, and immunohistochemical assessment of hippocampus structures of rats exposed to TCDD and high doses of tocopherol and acetylsalicylic acid.

Rosińczuk J, Dymarek R, Całkosiński I - Biomed Res Int (2015)

Bottom Line: The aim of the study was to determine the effect of dioxins on function, ultrastructure, and cytological and histological structure of hippocampus, particularly on expression of estrogen receptors in central nervous system as well as to define protective influence of tocopherol (TCP) and acetylsalicylic acid (ASA) on the decrease in activity of proinflammatory effects in central nervous system.Histopathological and ultrastructural image of hippocampus areas in rats, in which both TCP and ASA were used, is characterized by poorly expressed degenerative changes and smaller inflammatory reactivity.Using both TCP and ASA has a protective effect on functions of central nervous system.

View Article: PubMed Central - PubMed

Affiliation: Department of Nervous System Diseases, The Faculty of Health Science, Wroclaw Medical University, Bartla 5 Street, 51-618 Wrocław, Poland.

ABSTRACT
The effect of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) on central nervous system consists of changing expression of estrogen receptors, whereas the result of chronic inflammatory reaction caused by dioxin is occurrence of destructive changes in various organs connected with disturbed metabolism of connective tissue and damage of cells. The aim of the study was to determine the effect of dioxins on function, ultrastructure, and cytological and histological structure of hippocampus, particularly on expression of estrogen receptors in central nervous system as well as to define protective influence of tocopherol (TCP) and acetylsalicylic acid (ASA) on the decrease in activity of proinflammatory effects in central nervous system. It was shown that TCDD contributes to destructive and inflammatory changes along with demyelization of myelin sheaths and atrophy of estrogen receptors in hippocampus. Dioxin contributes to atrophy of estrogen receptors in hippocampus, in which also destructive and inflammatory changes were found along with demyelination of myelin sheaths. Histopathological and ultrastructural image of hippocampus areas in rats, in which both TCP and ASA were used, is characterized by poorly expressed degenerative changes and smaller inflammatory reactivity. Using both TCP and ASA has a protective effect on functions of central nervous system.

Show MeSH

Related in: MedlinePlus

Ultrastructural image of neurofibrils of hippocampus in group IP (enlargement 32 000x). The state of neuron degeneration has been found as well as vacuolation of endoplasmic reticulum (1) and mitochondria edema (2).
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4388018&req=5

fig20: Ultrastructural image of neurofibrils of hippocampus in group IP (enlargement 32 000x). The state of neuron degeneration has been found as well as vacuolation of endoplasmic reticulum (1) and mitochondria edema (2).

Mentions: In control group with induced inflammation of IP (Figure 20) in neuron perikaryon there are mitochondria being in different state of damage, while their edema prevails. Moreover mitochondria with correct structure are observed. Mitochondria with occurring edema are 30% of their population. The other part of perikaryon cytoplasm reveals basically correct structure; however areas of clarifications within the zone of endoplasmic reticulum can be found. The zone of granular reticulum mostly presents a correct image but in some places ectasias of rough endoplasmic reticulum can be observed. The above mentioned image is also confirmed in the area of axoplasm. Glia cells are unchanged. Dilation of space in nuclear areola is found. Significant changes in nerve fibers were not found. In some cells of oligodendroglia degenerative changes occur which consist in vacuolation of endoplasmic reticulum. In the area of capillary blood vessels vacuolation of endothelium cells can be found in some places as well as mitochondria edema. Macrophages are often visible in the zone adjacent to adventitia of capillary vessels. Endothelium cells of the above mentioned vessels are softened; in some of them the process of degeneration can be observed. Oligodendrocytes occurring near blood vessels do not reveal bigger changes in their structure. Some oligodendroglia cells adhering to blood vessels show symptoms of softening and some undergo degeneration.


Histopathological, ultrastructural, and immunohistochemical assessment of hippocampus structures of rats exposed to TCDD and high doses of tocopherol and acetylsalicylic acid.

Rosińczuk J, Dymarek R, Całkosiński I - Biomed Res Int (2015)

Ultrastructural image of neurofibrils of hippocampus in group IP (enlargement 32 000x). The state of neuron degeneration has been found as well as vacuolation of endoplasmic reticulum (1) and mitochondria edema (2).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4388018&req=5

fig20: Ultrastructural image of neurofibrils of hippocampus in group IP (enlargement 32 000x). The state of neuron degeneration has been found as well as vacuolation of endoplasmic reticulum (1) and mitochondria edema (2).
Mentions: In control group with induced inflammation of IP (Figure 20) in neuron perikaryon there are mitochondria being in different state of damage, while their edema prevails. Moreover mitochondria with correct structure are observed. Mitochondria with occurring edema are 30% of their population. The other part of perikaryon cytoplasm reveals basically correct structure; however areas of clarifications within the zone of endoplasmic reticulum can be found. The zone of granular reticulum mostly presents a correct image but in some places ectasias of rough endoplasmic reticulum can be observed. The above mentioned image is also confirmed in the area of axoplasm. Glia cells are unchanged. Dilation of space in nuclear areola is found. Significant changes in nerve fibers were not found. In some cells of oligodendroglia degenerative changes occur which consist in vacuolation of endoplasmic reticulum. In the area of capillary blood vessels vacuolation of endothelium cells can be found in some places as well as mitochondria edema. Macrophages are often visible in the zone adjacent to adventitia of capillary vessels. Endothelium cells of the above mentioned vessels are softened; in some of them the process of degeneration can be observed. Oligodendrocytes occurring near blood vessels do not reveal bigger changes in their structure. Some oligodendroglia cells adhering to blood vessels show symptoms of softening and some undergo degeneration.

Bottom Line: The aim of the study was to determine the effect of dioxins on function, ultrastructure, and cytological and histological structure of hippocampus, particularly on expression of estrogen receptors in central nervous system as well as to define protective influence of tocopherol (TCP) and acetylsalicylic acid (ASA) on the decrease in activity of proinflammatory effects in central nervous system.Histopathological and ultrastructural image of hippocampus areas in rats, in which both TCP and ASA were used, is characterized by poorly expressed degenerative changes and smaller inflammatory reactivity.Using both TCP and ASA has a protective effect on functions of central nervous system.

View Article: PubMed Central - PubMed

Affiliation: Department of Nervous System Diseases, The Faculty of Health Science, Wroclaw Medical University, Bartla 5 Street, 51-618 Wrocław, Poland.

ABSTRACT
The effect of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) on central nervous system consists of changing expression of estrogen receptors, whereas the result of chronic inflammatory reaction caused by dioxin is occurrence of destructive changes in various organs connected with disturbed metabolism of connective tissue and damage of cells. The aim of the study was to determine the effect of dioxins on function, ultrastructure, and cytological and histological structure of hippocampus, particularly on expression of estrogen receptors in central nervous system as well as to define protective influence of tocopherol (TCP) and acetylsalicylic acid (ASA) on the decrease in activity of proinflammatory effects in central nervous system. It was shown that TCDD contributes to destructive and inflammatory changes along with demyelization of myelin sheaths and atrophy of estrogen receptors in hippocampus. Dioxin contributes to atrophy of estrogen receptors in hippocampus, in which also destructive and inflammatory changes were found along with demyelination of myelin sheaths. Histopathological and ultrastructural image of hippocampus areas in rats, in which both TCP and ASA were used, is characterized by poorly expressed degenerative changes and smaller inflammatory reactivity. Using both TCP and ASA has a protective effect on functions of central nervous system.

Show MeSH
Related in: MedlinePlus