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Histopathological, ultrastructural, and immunohistochemical assessment of hippocampus structures of rats exposed to TCDD and high doses of tocopherol and acetylsalicylic acid.

Rosińczuk J, Dymarek R, Całkosiński I - Biomed Res Int (2015)

Bottom Line: The aim of the study was to determine the effect of dioxins on function, ultrastructure, and cytological and histological structure of hippocampus, particularly on expression of estrogen receptors in central nervous system as well as to define protective influence of tocopherol (TCP) and acetylsalicylic acid (ASA) on the decrease in activity of proinflammatory effects in central nervous system.Histopathological and ultrastructural image of hippocampus areas in rats, in which both TCP and ASA were used, is characterized by poorly expressed degenerative changes and smaller inflammatory reactivity.Using both TCP and ASA has a protective effect on functions of central nervous system.

View Article: PubMed Central - PubMed

Affiliation: Department of Nervous System Diseases, The Faculty of Health Science, Wroclaw Medical University, Bartla 5 Street, 51-618 Wrocław, Poland.

ABSTRACT
The effect of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) on central nervous system consists of changing expression of estrogen receptors, whereas the result of chronic inflammatory reaction caused by dioxin is occurrence of destructive changes in various organs connected with disturbed metabolism of connective tissue and damage of cells. The aim of the study was to determine the effect of dioxins on function, ultrastructure, and cytological and histological structure of hippocampus, particularly on expression of estrogen receptors in central nervous system as well as to define protective influence of tocopherol (TCP) and acetylsalicylic acid (ASA) on the decrease in activity of proinflammatory effects in central nervous system. It was shown that TCDD contributes to destructive and inflammatory changes along with demyelization of myelin sheaths and atrophy of estrogen receptors in hippocampus. Dioxin contributes to atrophy of estrogen receptors in hippocampus, in which also destructive and inflammatory changes were found along with demyelination of myelin sheaths. Histopathological and ultrastructural image of hippocampus areas in rats, in which both TCP and ASA were used, is characterized by poorly expressed degenerative changes and smaller inflammatory reactivity. Using both TCP and ASA has a protective effect on functions of central nervous system.

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Related in: MedlinePlus

Histological image of hippocampus in animals in group IPDAE (enlargement 400x). Visible infiltration around blood vessels consisting of macrophages and microglia cells (1); sparse ganglion cells show neuron degradation states; however the whole image looks more favorable than in the previous experimental groups; spongy structure is more poorly expressed (2).
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fig6: Histological image of hippocampus in animals in group IPDAE (enlargement 400x). Visible infiltration around blood vessels consisting of macrophages and microglia cells (1); sparse ganglion cells show neuron degradation states; however the whole image looks more favorable than in the previous experimental groups; spongy structure is more poorly expressed (2).

Mentions: In Ammon's horn in group IPDAE (Figure 6) main changes concern the zone of pyramidal cells; to a smaller extent they apply to molecular layer and granular cells. The above mentioned changes most clearly are emphasized in direct and close vicinity of blood vessels. Cells of pyramidal layer reveal cytoplasmic vacuolation of perikaryon. In many cells of that layer, nuclei reveal karyopyknosis and beginnings of degenerative changes. Similar changes but of bigger intensity occur in granular cells. Granular cells in histological image present poorly expressed foam structure, which is the result of going on process of degeneration. In molecular layer there are smaller degree degenerative processes. Besides going on process of degeneration one can observe glial proliferation of microglia cells and oligodendroglia, which is particularly visible around blood vessels.


Histopathological, ultrastructural, and immunohistochemical assessment of hippocampus structures of rats exposed to TCDD and high doses of tocopherol and acetylsalicylic acid.

Rosińczuk J, Dymarek R, Całkosiński I - Biomed Res Int (2015)

Histological image of hippocampus in animals in group IPDAE (enlargement 400x). Visible infiltration around blood vessels consisting of macrophages and microglia cells (1); sparse ganglion cells show neuron degradation states; however the whole image looks more favorable than in the previous experimental groups; spongy structure is more poorly expressed (2).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4388018&req=5

fig6: Histological image of hippocampus in animals in group IPDAE (enlargement 400x). Visible infiltration around blood vessels consisting of macrophages and microglia cells (1); sparse ganglion cells show neuron degradation states; however the whole image looks more favorable than in the previous experimental groups; spongy structure is more poorly expressed (2).
Mentions: In Ammon's horn in group IPDAE (Figure 6) main changes concern the zone of pyramidal cells; to a smaller extent they apply to molecular layer and granular cells. The above mentioned changes most clearly are emphasized in direct and close vicinity of blood vessels. Cells of pyramidal layer reveal cytoplasmic vacuolation of perikaryon. In many cells of that layer, nuclei reveal karyopyknosis and beginnings of degenerative changes. Similar changes but of bigger intensity occur in granular cells. Granular cells in histological image present poorly expressed foam structure, which is the result of going on process of degeneration. In molecular layer there are smaller degree degenerative processes. Besides going on process of degeneration one can observe glial proliferation of microglia cells and oligodendroglia, which is particularly visible around blood vessels.

Bottom Line: The aim of the study was to determine the effect of dioxins on function, ultrastructure, and cytological and histological structure of hippocampus, particularly on expression of estrogen receptors in central nervous system as well as to define protective influence of tocopherol (TCP) and acetylsalicylic acid (ASA) on the decrease in activity of proinflammatory effects in central nervous system.Histopathological and ultrastructural image of hippocampus areas in rats, in which both TCP and ASA were used, is characterized by poorly expressed degenerative changes and smaller inflammatory reactivity.Using both TCP and ASA has a protective effect on functions of central nervous system.

View Article: PubMed Central - PubMed

Affiliation: Department of Nervous System Diseases, The Faculty of Health Science, Wroclaw Medical University, Bartla 5 Street, 51-618 Wrocław, Poland.

ABSTRACT
The effect of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) on central nervous system consists of changing expression of estrogen receptors, whereas the result of chronic inflammatory reaction caused by dioxin is occurrence of destructive changes in various organs connected with disturbed metabolism of connective tissue and damage of cells. The aim of the study was to determine the effect of dioxins on function, ultrastructure, and cytological and histological structure of hippocampus, particularly on expression of estrogen receptors in central nervous system as well as to define protective influence of tocopherol (TCP) and acetylsalicylic acid (ASA) on the decrease in activity of proinflammatory effects in central nervous system. It was shown that TCDD contributes to destructive and inflammatory changes along with demyelization of myelin sheaths and atrophy of estrogen receptors in hippocampus. Dioxin contributes to atrophy of estrogen receptors in hippocampus, in which also destructive and inflammatory changes were found along with demyelination of myelin sheaths. Histopathological and ultrastructural image of hippocampus areas in rats, in which both TCP and ASA were used, is characterized by poorly expressed degenerative changes and smaller inflammatory reactivity. Using both TCP and ASA has a protective effect on functions of central nervous system.

Show MeSH
Related in: MedlinePlus