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Histopathological, ultrastructural, and immunohistochemical assessment of hippocampus structures of rats exposed to TCDD and high doses of tocopherol and acetylsalicylic acid.

Rosińczuk J, Dymarek R, Całkosiński I - Biomed Res Int (2015)

Bottom Line: The aim of the study was to determine the effect of dioxins on function, ultrastructure, and cytological and histological structure of hippocampus, particularly on expression of estrogen receptors in central nervous system as well as to define protective influence of tocopherol (TCP) and acetylsalicylic acid (ASA) on the decrease in activity of proinflammatory effects in central nervous system.Histopathological and ultrastructural image of hippocampus areas in rats, in which both TCP and ASA were used, is characterized by poorly expressed degenerative changes and smaller inflammatory reactivity.Using both TCP and ASA has a protective effect on functions of central nervous system.

View Article: PubMed Central - PubMed

Affiliation: Department of Nervous System Diseases, The Faculty of Health Science, Wroclaw Medical University, Bartla 5 Street, 51-618 Wrocław, Poland.

ABSTRACT
The effect of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) on central nervous system consists of changing expression of estrogen receptors, whereas the result of chronic inflammatory reaction caused by dioxin is occurrence of destructive changes in various organs connected with disturbed metabolism of connective tissue and damage of cells. The aim of the study was to determine the effect of dioxins on function, ultrastructure, and cytological and histological structure of hippocampus, particularly on expression of estrogen receptors in central nervous system as well as to define protective influence of tocopherol (TCP) and acetylsalicylic acid (ASA) on the decrease in activity of proinflammatory effects in central nervous system. It was shown that TCDD contributes to destructive and inflammatory changes along with demyelization of myelin sheaths and atrophy of estrogen receptors in hippocampus. Dioxin contributes to atrophy of estrogen receptors in hippocampus, in which also destructive and inflammatory changes were found along with demyelination of myelin sheaths. Histopathological and ultrastructural image of hippocampus areas in rats, in which both TCP and ASA were used, is characterized by poorly expressed degenerative changes and smaller inflammatory reactivity. Using both TCP and ASA has a protective effect on functions of central nervous system.

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Related in: MedlinePlus

Histological image of hippocampus in animals in group IPDA (enlargement 400x). In the lumen of capillary vessels, there are numerous erythrocytes visible in rouleau formation (1), which may indicate the presence of venostasis; a small concentration of macrophages and microglia cells is observed in perivascular zone (2); in some ganglion cells, degradation states are found in the form of cytoplasmic vacuolation (3).
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fig5: Histological image of hippocampus in animals in group IPDA (enlargement 400x). In the lumen of capillary vessels, there are numerous erythrocytes visible in rouleau formation (1), which may indicate the presence of venostasis; a small concentration of macrophages and microglia cells is observed in perivascular zone (2); in some ganglion cells, degradation states are found in the form of cytoplasmic vacuolation (3).

Mentions: In Ammon's horn in group IPDA (Figure 5) damage of grey matter can be found to a smaller extent. Fundamental changes occur in pyramidal cells, to a smaller extent in molecular layer and cells of granular layer. Those changes are clearly emphasized near blood vessels and around them. In perikaryons of ganglionic cells cytoplasmic vacuolation is observed. It takes the form of foam structure. Granular cells also present foam structure,; however the scope of those changes is smaller than in pyramidal cells. Degenerative changes of nerve cells are accompanied by glial proliferation, especially of oligodendroglia and microglia cells. Microglia cells occur most clearly around blood vessels. A degree of damage of blood vessels is smaller than damage occurring in group IPD. Only in white matter the degree of damage of blood vessels stays at similar level like in animals, which did not receive salicylate.


Histopathological, ultrastructural, and immunohistochemical assessment of hippocampus structures of rats exposed to TCDD and high doses of tocopherol and acetylsalicylic acid.

Rosińczuk J, Dymarek R, Całkosiński I - Biomed Res Int (2015)

Histological image of hippocampus in animals in group IPDA (enlargement 400x). In the lumen of capillary vessels, there are numerous erythrocytes visible in rouleau formation (1), which may indicate the presence of venostasis; a small concentration of macrophages and microglia cells is observed in perivascular zone (2); in some ganglion cells, degradation states are found in the form of cytoplasmic vacuolation (3).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4388018&req=5

fig5: Histological image of hippocampus in animals in group IPDA (enlargement 400x). In the lumen of capillary vessels, there are numerous erythrocytes visible in rouleau formation (1), which may indicate the presence of venostasis; a small concentration of macrophages and microglia cells is observed in perivascular zone (2); in some ganglion cells, degradation states are found in the form of cytoplasmic vacuolation (3).
Mentions: In Ammon's horn in group IPDA (Figure 5) damage of grey matter can be found to a smaller extent. Fundamental changes occur in pyramidal cells, to a smaller extent in molecular layer and cells of granular layer. Those changes are clearly emphasized near blood vessels and around them. In perikaryons of ganglionic cells cytoplasmic vacuolation is observed. It takes the form of foam structure. Granular cells also present foam structure,; however the scope of those changes is smaller than in pyramidal cells. Degenerative changes of nerve cells are accompanied by glial proliferation, especially of oligodendroglia and microglia cells. Microglia cells occur most clearly around blood vessels. A degree of damage of blood vessels is smaller than damage occurring in group IPD. Only in white matter the degree of damage of blood vessels stays at similar level like in animals, which did not receive salicylate.

Bottom Line: The aim of the study was to determine the effect of dioxins on function, ultrastructure, and cytological and histological structure of hippocampus, particularly on expression of estrogen receptors in central nervous system as well as to define protective influence of tocopherol (TCP) and acetylsalicylic acid (ASA) on the decrease in activity of proinflammatory effects in central nervous system.Histopathological and ultrastructural image of hippocampus areas in rats, in which both TCP and ASA were used, is characterized by poorly expressed degenerative changes and smaller inflammatory reactivity.Using both TCP and ASA has a protective effect on functions of central nervous system.

View Article: PubMed Central - PubMed

Affiliation: Department of Nervous System Diseases, The Faculty of Health Science, Wroclaw Medical University, Bartla 5 Street, 51-618 Wrocław, Poland.

ABSTRACT
The effect of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) on central nervous system consists of changing expression of estrogen receptors, whereas the result of chronic inflammatory reaction caused by dioxin is occurrence of destructive changes in various organs connected with disturbed metabolism of connective tissue and damage of cells. The aim of the study was to determine the effect of dioxins on function, ultrastructure, and cytological and histological structure of hippocampus, particularly on expression of estrogen receptors in central nervous system as well as to define protective influence of tocopherol (TCP) and acetylsalicylic acid (ASA) on the decrease in activity of proinflammatory effects in central nervous system. It was shown that TCDD contributes to destructive and inflammatory changes along with demyelization of myelin sheaths and atrophy of estrogen receptors in hippocampus. Dioxin contributes to atrophy of estrogen receptors in hippocampus, in which also destructive and inflammatory changes were found along with demyelination of myelin sheaths. Histopathological and ultrastructural image of hippocampus areas in rats, in which both TCP and ASA were used, is characterized by poorly expressed degenerative changes and smaller inflammatory reactivity. Using both TCP and ASA has a protective effect on functions of central nervous system.

Show MeSH
Related in: MedlinePlus