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Histopathological, ultrastructural, and immunohistochemical assessment of hippocampus structures of rats exposed to TCDD and high doses of tocopherol and acetylsalicylic acid.

Rosińczuk J, Dymarek R, Całkosiński I - Biomed Res Int (2015)

Bottom Line: The aim of the study was to determine the effect of dioxins on function, ultrastructure, and cytological and histological structure of hippocampus, particularly on expression of estrogen receptors in central nervous system as well as to define protective influence of tocopherol (TCP) and acetylsalicylic acid (ASA) on the decrease in activity of proinflammatory effects in central nervous system.Histopathological and ultrastructural image of hippocampus areas in rats, in which both TCP and ASA were used, is characterized by poorly expressed degenerative changes and smaller inflammatory reactivity.Using both TCP and ASA has a protective effect on functions of central nervous system.

View Article: PubMed Central - PubMed

Affiliation: Department of Nervous System Diseases, The Faculty of Health Science, Wroclaw Medical University, Bartla 5 Street, 51-618 Wrocław, Poland.

ABSTRACT
The effect of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) on central nervous system consists of changing expression of estrogen receptors, whereas the result of chronic inflammatory reaction caused by dioxin is occurrence of destructive changes in various organs connected with disturbed metabolism of connective tissue and damage of cells. The aim of the study was to determine the effect of dioxins on function, ultrastructure, and cytological and histological structure of hippocampus, particularly on expression of estrogen receptors in central nervous system as well as to define protective influence of tocopherol (TCP) and acetylsalicylic acid (ASA) on the decrease in activity of proinflammatory effects in central nervous system. It was shown that TCDD contributes to destructive and inflammatory changes along with demyelization of myelin sheaths and atrophy of estrogen receptors in hippocampus. Dioxin contributes to atrophy of estrogen receptors in hippocampus, in which also destructive and inflammatory changes were found along with demyelination of myelin sheaths. Histopathological and ultrastructural image of hippocampus areas in rats, in which both TCP and ASA were used, is characterized by poorly expressed degenerative changes and smaller inflammatory reactivity. Using both TCP and ASA has a protective effect on functions of central nervous system.

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Histological image of hippocampus in animals in group IPD (enlargement 400x). Neurodegradation states of pyramidal neurons (1) and granule cells (2) are demonstrated; around blood vessels there is infiltration consisting of microglia cells and macrophages (3); in the area between nerve cells histological image presents foam structures (4).
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fig3: Histological image of hippocampus in animals in group IPD (enlargement 400x). Neurodegradation states of pyramidal neurons (1) and granule cells (2) are demonstrated; around blood vessels there is infiltration consisting of microglia cells and macrophages (3); in the area between nerve cells histological image presents foam structures (4).

Mentions: In Ammon's horn in group IPD (Figure 3) there is high degree damage of grey matter of hippocampus; particularly clear degenerative changes can be seen in pyramidal cells and smaller ones in cells of granular and molecular layer. Delaminations between pyramidal cells and adjacent layers are confirmed. In particular pyramidal cells karyopyknosis of testicles can be seen as well as translucency of perikaryon cytoplasm. It also concerns the deviation zone of neuron processes. In a near zone (of analyzed neurons) proliferation of glia cells can be noticed. Degenerative changes in the above mentioned layers consist mainly in various degree of cytoplasmic vacuolation, which result in foam structure. Both in grey and in white zone there are changes displaying clamping of blood vessel lumen. In perivascular zones there is infiltration consisting of microglia cells and macrophages. Glial proliferation also applies to oligodendroglia cells, to a smaller extent to astroglia. A smaller degree of damage was found in molecular and granular layers.


Histopathological, ultrastructural, and immunohistochemical assessment of hippocampus structures of rats exposed to TCDD and high doses of tocopherol and acetylsalicylic acid.

Rosińczuk J, Dymarek R, Całkosiński I - Biomed Res Int (2015)

Histological image of hippocampus in animals in group IPD (enlargement 400x). Neurodegradation states of pyramidal neurons (1) and granule cells (2) are demonstrated; around blood vessels there is infiltration consisting of microglia cells and macrophages (3); in the area between nerve cells histological image presents foam structures (4).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4388018&req=5

fig3: Histological image of hippocampus in animals in group IPD (enlargement 400x). Neurodegradation states of pyramidal neurons (1) and granule cells (2) are demonstrated; around blood vessels there is infiltration consisting of microglia cells and macrophages (3); in the area between nerve cells histological image presents foam structures (4).
Mentions: In Ammon's horn in group IPD (Figure 3) there is high degree damage of grey matter of hippocampus; particularly clear degenerative changes can be seen in pyramidal cells and smaller ones in cells of granular and molecular layer. Delaminations between pyramidal cells and adjacent layers are confirmed. In particular pyramidal cells karyopyknosis of testicles can be seen as well as translucency of perikaryon cytoplasm. It also concerns the deviation zone of neuron processes. In a near zone (of analyzed neurons) proliferation of glia cells can be noticed. Degenerative changes in the above mentioned layers consist mainly in various degree of cytoplasmic vacuolation, which result in foam structure. Both in grey and in white zone there are changes displaying clamping of blood vessel lumen. In perivascular zones there is infiltration consisting of microglia cells and macrophages. Glial proliferation also applies to oligodendroglia cells, to a smaller extent to astroglia. A smaller degree of damage was found in molecular and granular layers.

Bottom Line: The aim of the study was to determine the effect of dioxins on function, ultrastructure, and cytological and histological structure of hippocampus, particularly on expression of estrogen receptors in central nervous system as well as to define protective influence of tocopherol (TCP) and acetylsalicylic acid (ASA) on the decrease in activity of proinflammatory effects in central nervous system.Histopathological and ultrastructural image of hippocampus areas in rats, in which both TCP and ASA were used, is characterized by poorly expressed degenerative changes and smaller inflammatory reactivity.Using both TCP and ASA has a protective effect on functions of central nervous system.

View Article: PubMed Central - PubMed

Affiliation: Department of Nervous System Diseases, The Faculty of Health Science, Wroclaw Medical University, Bartla 5 Street, 51-618 Wrocław, Poland.

ABSTRACT
The effect of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) on central nervous system consists of changing expression of estrogen receptors, whereas the result of chronic inflammatory reaction caused by dioxin is occurrence of destructive changes in various organs connected with disturbed metabolism of connective tissue and damage of cells. The aim of the study was to determine the effect of dioxins on function, ultrastructure, and cytological and histological structure of hippocampus, particularly on expression of estrogen receptors in central nervous system as well as to define protective influence of tocopherol (TCP) and acetylsalicylic acid (ASA) on the decrease in activity of proinflammatory effects in central nervous system. It was shown that TCDD contributes to destructive and inflammatory changes along with demyelization of myelin sheaths and atrophy of estrogen receptors in hippocampus. Dioxin contributes to atrophy of estrogen receptors in hippocampus, in which also destructive and inflammatory changes were found along with demyelination of myelin sheaths. Histopathological and ultrastructural image of hippocampus areas in rats, in which both TCP and ASA were used, is characterized by poorly expressed degenerative changes and smaller inflammatory reactivity. Using both TCP and ASA has a protective effect on functions of central nervous system.

Show MeSH
Related in: MedlinePlus