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Histopathological, ultrastructural, and immunohistochemical assessment of hippocampus structures of rats exposed to TCDD and high doses of tocopherol and acetylsalicylic acid.

Rosińczuk J, Dymarek R, Całkosiński I - Biomed Res Int (2015)

Bottom Line: The aim of the study was to determine the effect of dioxins on function, ultrastructure, and cytological and histological structure of hippocampus, particularly on expression of estrogen receptors in central nervous system as well as to define protective influence of tocopherol (TCP) and acetylsalicylic acid (ASA) on the decrease in activity of proinflammatory effects in central nervous system.Histopathological and ultrastructural image of hippocampus areas in rats, in which both TCP and ASA were used, is characterized by poorly expressed degenerative changes and smaller inflammatory reactivity.Using both TCP and ASA has a protective effect on functions of central nervous system.

View Article: PubMed Central - PubMed

Affiliation: Department of Nervous System Diseases, The Faculty of Health Science, Wroclaw Medical University, Bartla 5 Street, 51-618 Wrocław, Poland.

ABSTRACT
The effect of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) on central nervous system consists of changing expression of estrogen receptors, whereas the result of chronic inflammatory reaction caused by dioxin is occurrence of destructive changes in various organs connected with disturbed metabolism of connective tissue and damage of cells. The aim of the study was to determine the effect of dioxins on function, ultrastructure, and cytological and histological structure of hippocampus, particularly on expression of estrogen receptors in central nervous system as well as to define protective influence of tocopherol (TCP) and acetylsalicylic acid (ASA) on the decrease in activity of proinflammatory effects in central nervous system. It was shown that TCDD contributes to destructive and inflammatory changes along with demyelization of myelin sheaths and atrophy of estrogen receptors in hippocampus. Dioxin contributes to atrophy of estrogen receptors in hippocampus, in which also destructive and inflammatory changes were found along with demyelination of myelin sheaths. Histopathological and ultrastructural image of hippocampus areas in rats, in which both TCP and ASA were used, is characterized by poorly expressed degenerative changes and smaller inflammatory reactivity. Using both TCP and ASA has a protective effect on functions of central nervous system.

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Histological image of hippocampus in animals in group IP (enlargement 400x). Nearby blood vessels, cells of microglia and macrophages agglomerate. Large multipolar neurons (1) are in the state of dispersion; between them there are smaller neurons (2) and cells of astroglia and oligodendroglia (3); nearby blood vessels there are microphages and macrophages present (micromesoglia cells); relaxation of weaving between neurons is noteworthy (4).
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fig2: Histological image of hippocampus in animals in group IP (enlargement 400x). Nearby blood vessels, cells of microglia and macrophages agglomerate. Large multipolar neurons (1) are in the state of dispersion; between them there are smaller neurons (2) and cells of astroglia and oligodendroglia (3); nearby blood vessels there are microphages and macrophages present (micromesoglia cells); relaxation of weaving between neurons is noteworthy (4).

Mentions: In the control group with induced inflammation IP (Figure 2) the clearest reaction concentrates around blood vessels. In those vessels one can notice slower blood flow, which is proved by retention of blood cells in the lumen of vessels in many places. Around blood vessels there is bigger concentration of microglia cells. Moreover glial proliferation was found. In those places dilutions of neuron weaving are visible and in some nerve cells in the area of perikaryon degeneration changes can be seen. Those changes mainly concern the area of granular nerve cells. To a smaller extent the above mentioned changes affect the layer of pyramidal cells. Vascular changes have a similar nature.


Histopathological, ultrastructural, and immunohistochemical assessment of hippocampus structures of rats exposed to TCDD and high doses of tocopherol and acetylsalicylic acid.

Rosińczuk J, Dymarek R, Całkosiński I - Biomed Res Int (2015)

Histological image of hippocampus in animals in group IP (enlargement 400x). Nearby blood vessels, cells of microglia and macrophages agglomerate. Large multipolar neurons (1) are in the state of dispersion; between them there are smaller neurons (2) and cells of astroglia and oligodendroglia (3); nearby blood vessels there are microphages and macrophages present (micromesoglia cells); relaxation of weaving between neurons is noteworthy (4).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4388018&req=5

fig2: Histological image of hippocampus in animals in group IP (enlargement 400x). Nearby blood vessels, cells of microglia and macrophages agglomerate. Large multipolar neurons (1) are in the state of dispersion; between them there are smaller neurons (2) and cells of astroglia and oligodendroglia (3); nearby blood vessels there are microphages and macrophages present (micromesoglia cells); relaxation of weaving between neurons is noteworthy (4).
Mentions: In the control group with induced inflammation IP (Figure 2) the clearest reaction concentrates around blood vessels. In those vessels one can notice slower blood flow, which is proved by retention of blood cells in the lumen of vessels in many places. Around blood vessels there is bigger concentration of microglia cells. Moreover glial proliferation was found. In those places dilutions of neuron weaving are visible and in some nerve cells in the area of perikaryon degeneration changes can be seen. Those changes mainly concern the area of granular nerve cells. To a smaller extent the above mentioned changes affect the layer of pyramidal cells. Vascular changes have a similar nature.

Bottom Line: The aim of the study was to determine the effect of dioxins on function, ultrastructure, and cytological and histological structure of hippocampus, particularly on expression of estrogen receptors in central nervous system as well as to define protective influence of tocopherol (TCP) and acetylsalicylic acid (ASA) on the decrease in activity of proinflammatory effects in central nervous system.Histopathological and ultrastructural image of hippocampus areas in rats, in which both TCP and ASA were used, is characterized by poorly expressed degenerative changes and smaller inflammatory reactivity.Using both TCP and ASA has a protective effect on functions of central nervous system.

View Article: PubMed Central - PubMed

Affiliation: Department of Nervous System Diseases, The Faculty of Health Science, Wroclaw Medical University, Bartla 5 Street, 51-618 Wrocław, Poland.

ABSTRACT
The effect of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) on central nervous system consists of changing expression of estrogen receptors, whereas the result of chronic inflammatory reaction caused by dioxin is occurrence of destructive changes in various organs connected with disturbed metabolism of connective tissue and damage of cells. The aim of the study was to determine the effect of dioxins on function, ultrastructure, and cytological and histological structure of hippocampus, particularly on expression of estrogen receptors in central nervous system as well as to define protective influence of tocopherol (TCP) and acetylsalicylic acid (ASA) on the decrease in activity of proinflammatory effects in central nervous system. It was shown that TCDD contributes to destructive and inflammatory changes along with demyelization of myelin sheaths and atrophy of estrogen receptors in hippocampus. Dioxin contributes to atrophy of estrogen receptors in hippocampus, in which also destructive and inflammatory changes were found along with demyelination of myelin sheaths. Histopathological and ultrastructural image of hippocampus areas in rats, in which both TCP and ASA were used, is characterized by poorly expressed degenerative changes and smaller inflammatory reactivity. Using both TCP and ASA has a protective effect on functions of central nervous system.

Show MeSH
Related in: MedlinePlus