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Role of bone marrow derived pluripotent stem cells in peripheral nerve repair in adult rats: A morphometric evaluation.

Kurwale NS, Suri V, Srivastava A, Suri A, Mohanti S, Yadav P, Sharma MC, Sarkar C - J Neurosci Rural Pract (2015 Apr-Jun)

Bottom Line: At 15 days there was a statistically significant difference found in mean axon diameter, mean nerve thickness and myelin thickness at the repair site (P < 0.05).At 60 days, the difference in above-mentioned parameters was statistically significant at the distal most sites.Transplanting BM-MNCS at the site of peripheral nerve injury leads to significantly better recovery.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurosurgery, All India Institute of Medical Sciences, New Delhi, India.

ABSTRACT

Objectives: Semi-quantitative and quantitative assessment of the effect of bone marrow-derived mononuclear cells (BM-MNC) on early and late phase of nerve regeneration in rat sciatic nerve model.

Materials and methods: Sciatic nerve transection and repair was performed in 50 inbred female Wistar albino rats divided equally in two groups. In the test group the gap was filled with BM-MNCs obtained from the two male rats and fibrin sealant, while in the control group only fibrin sealant was used. Sciatic nerve was harvested at 15 days and at 60 days interval. Parameters of regeneration were assessed at anastomosis (G), intermediate distal (C), and distal site (A). Semi-quantitative (histopathological) and quantitative (morphometric) parameters were analyzed.

Results: At 15 days there was a statistically significant difference found in mean axon diameter, mean nerve thickness and myelin thickness at the repair site (P < 0.05). However, in the distal areas, the axons were sparse and myelin rings were very thin in both the groups. At 60 days, the difference in above-mentioned parameters was statistically significant at the distal most sites. FISH assay confirmed the presence of Y chromosome, confirming the presence of BM-MNCs from the male rats.

Conclusions: Transplanting BM-MNCS at the site of peripheral nerve injury leads to significantly better recovery. These differences were evident at the repair site and at the intermediate distal site at 15 days and at the distal most sites at 60 days. With practically no ethical issue regarding their isolation and application, they can be easily used for clinical trials.

No MeSH data available.


Related in: MedlinePlus

(a) At the proximal site, the test case showing good density of axons which are well oriented with single Schwann cells at the periphery, HE ×400 (b) majority of axons are large in size, NF ×400 and (c) thickly myelinated, Loyez ×400 (d) in case of control, axons are also in good density, HE ×400, (e) majority of axons are large, NF ×400 and (f) thickly myelinated, Loyez ×400
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Figure 4: (a) At the proximal site, the test case showing good density of axons which are well oriented with single Schwann cells at the periphery, HE ×400 (b) majority of axons are large in size, NF ×400 and (c) thickly myelinated, Loyez ×400 (d) in case of control, axons are also in good density, HE ×400, (e) majority of axons are large, NF ×400 and (f) thickly myelinated, Loyez ×400

Mentions: In both the groups a very small percentage of myelinated fibers were seen in the distal areas ‘A’ and ‘C’. At the distal most site ‘A’, test as well as control group showed thin myelinated axons and the density of myelinated axons in control ranged from 5% to 35%. In the test group there were thin myelinated axons and the density ranged from 15% to 60%. However, remarkable difference was observed at the repair site in two groups. All the cases with proximal sites ‘D’ and ‘F’ had uniformly dense myelinated fibers majority of which had uniform thickly myelinated rings [Figure 4].


Role of bone marrow derived pluripotent stem cells in peripheral nerve repair in adult rats: A morphometric evaluation.

Kurwale NS, Suri V, Srivastava A, Suri A, Mohanti S, Yadav P, Sharma MC, Sarkar C - J Neurosci Rural Pract (2015 Apr-Jun)

(a) At the proximal site, the test case showing good density of axons which are well oriented with single Schwann cells at the periphery, HE ×400 (b) majority of axons are large in size, NF ×400 and (c) thickly myelinated, Loyez ×400 (d) in case of control, axons are also in good density, HE ×400, (e) majority of axons are large, NF ×400 and (f) thickly myelinated, Loyez ×400
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4387802&req=5

Figure 4: (a) At the proximal site, the test case showing good density of axons which are well oriented with single Schwann cells at the periphery, HE ×400 (b) majority of axons are large in size, NF ×400 and (c) thickly myelinated, Loyez ×400 (d) in case of control, axons are also in good density, HE ×400, (e) majority of axons are large, NF ×400 and (f) thickly myelinated, Loyez ×400
Mentions: In both the groups a very small percentage of myelinated fibers were seen in the distal areas ‘A’ and ‘C’. At the distal most site ‘A’, test as well as control group showed thin myelinated axons and the density of myelinated axons in control ranged from 5% to 35%. In the test group there were thin myelinated axons and the density ranged from 15% to 60%. However, remarkable difference was observed at the repair site in two groups. All the cases with proximal sites ‘D’ and ‘F’ had uniformly dense myelinated fibers majority of which had uniform thickly myelinated rings [Figure 4].

Bottom Line: At 15 days there was a statistically significant difference found in mean axon diameter, mean nerve thickness and myelin thickness at the repair site (P < 0.05).At 60 days, the difference in above-mentioned parameters was statistically significant at the distal most sites.Transplanting BM-MNCS at the site of peripheral nerve injury leads to significantly better recovery.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurosurgery, All India Institute of Medical Sciences, New Delhi, India.

ABSTRACT

Objectives: Semi-quantitative and quantitative assessment of the effect of bone marrow-derived mononuclear cells (BM-MNC) on early and late phase of nerve regeneration in rat sciatic nerve model.

Materials and methods: Sciatic nerve transection and repair was performed in 50 inbred female Wistar albino rats divided equally in two groups. In the test group the gap was filled with BM-MNCs obtained from the two male rats and fibrin sealant, while in the control group only fibrin sealant was used. Sciatic nerve was harvested at 15 days and at 60 days interval. Parameters of regeneration were assessed at anastomosis (G), intermediate distal (C), and distal site (A). Semi-quantitative (histopathological) and quantitative (morphometric) parameters were analyzed.

Results: At 15 days there was a statistically significant difference found in mean axon diameter, mean nerve thickness and myelin thickness at the repair site (P < 0.05). However, in the distal areas, the axons were sparse and myelin rings were very thin in both the groups. At 60 days, the difference in above-mentioned parameters was statistically significant at the distal most sites. FISH assay confirmed the presence of Y chromosome, confirming the presence of BM-MNCs from the male rats.

Conclusions: Transplanting BM-MNCS at the site of peripheral nerve injury leads to significantly better recovery. These differences were evident at the repair site and at the intermediate distal site at 15 days and at the distal most sites at 60 days. With practically no ethical issue regarding their isolation and application, they can be easily used for clinical trials.

No MeSH data available.


Related in: MedlinePlus