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Cytotoxicity evaluation of a copaiba oil-based root canal sealer compared to three commonly used sealers in endodontics.

Garrido AD, de Cara SP, Marques MM, Sponchiado EC, Garcia Lda F, de Sousa-Neto MD - Dent Res J (Isfahan) (2015 Mar-Apr)

Bottom Line: The aim of this study was to evaluate the cytotoxic effect of a new Copaiba oil-based root canal sealer (Biosealer [BS]) on osteoblast-like Osteo-1 cells.S26, EF and AHP presented decreased cellular viability considerably, with statistical significance compared with CG (P < 0.05).The Copaiba oil-based root canal sealer presented promising results in terms of cytotoxicity which indicated its usefulness as a root canal sealer.

View Article: PubMed Central - PubMed

Affiliation: Department of Endodontics, School of Dentistry, Federal University of Amazonas, Manaus, Amazonas, Brazil.

ABSTRACT

Background: The constant development of new root canal sealers has allowed the solution of a large number of clinical cases in endodontics, however, cytotoxicity of such sealers must be tested before their validation as filling materials. The aim of this study was to evaluate the cytotoxic effect of a new Copaiba oil-based root canal sealer (Biosealer [BS]) on osteoblast-like Osteo-1 cells.

Materials and methods: The experimental groups were formed according to the culture medium conditioned with the tested sealers, as follows: Control group (CG) (culture medium without conditioning); Sealer 26 (S26) - culture medium + S26; Endofill (EF) - culture medium + EF; AH Plus (AHP) - culture medium + AHP; and BS - culture medium + BS (Copaiba oil-based sealer). The conditioned culture medium was placed in contact with 2 × 10(4) cells cultivated on 60 mm diameter Petri dishes for 24 h. Then, hemocytometer count was performed to evaluate cellular viability, using Trypan Blue assay. The normal distribution of data was tested by the Kolmogorov-Smirnov test and the values obtained for cellular viability were statistically analyzed (1-way ANOVA, Tukey's test - P < 0.05), with a significance level of 5%.

Results: S26, EF and AHP presented decreased cellular viability considerably, with statistical significance compared with CG (P < 0.05). BS maintained cellular viability similar to CG (P > 0.05).

Conclusion: The Copaiba oil-based root canal sealer presented promising results in terms of cytotoxicity which indicated its usefulness as a root canal sealer.

No MeSH data available.


Related in: MedlinePlus

Cells counting procedure - photomicrographs of the Neubauer Chamber on inverted phase microscope. Live cells appear in suspension, colorless and bright (refractile) under phase contrast (arrow). Dead cells, non-viable, are stained with Trypan Blue and are non-refractile. (a) Control group, (b) sealer 26, (c) Endofill, (d) AH Plus and (e) Biosealer (×100)
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Figure 2: Cells counting procedure - photomicrographs of the Neubauer Chamber on inverted phase microscope. Live cells appear in suspension, colorless and bright (refractile) under phase contrast (arrow). Dead cells, non-viable, are stained with Trypan Blue and are non-refractile. (a) Control group, (b) sealer 26, (c) Endofill, (d) AH Plus and (e) Biosealer (×100)

Mentions: Figures 1 and 2 present the cellular viability mean values and the comparison of data among the different groups. It could be observed that there was statistically significant difference among the groups (P < 0.05). The cell viability of CG (95.02 ± 1.95) was statistically different from S26 (2.77 ± 2.37), AHP (5.98 ± 5.13) and EF (76.70 ± 7.60). The results in GC were statistically similar - to BS (95.36 ± 1.53). Furthermore, S26 and AHP presented the lowest cellular viability mean values in comparison with the other groups.


Cytotoxicity evaluation of a copaiba oil-based root canal sealer compared to three commonly used sealers in endodontics.

Garrido AD, de Cara SP, Marques MM, Sponchiado EC, Garcia Lda F, de Sousa-Neto MD - Dent Res J (Isfahan) (2015 Mar-Apr)

Cells counting procedure - photomicrographs of the Neubauer Chamber on inverted phase microscope. Live cells appear in suspension, colorless and bright (refractile) under phase contrast (arrow). Dead cells, non-viable, are stained with Trypan Blue and are non-refractile. (a) Control group, (b) sealer 26, (c) Endofill, (d) AH Plus and (e) Biosealer (×100)
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4387623&req=5

Figure 2: Cells counting procedure - photomicrographs of the Neubauer Chamber on inverted phase microscope. Live cells appear in suspension, colorless and bright (refractile) under phase contrast (arrow). Dead cells, non-viable, are stained with Trypan Blue and are non-refractile. (a) Control group, (b) sealer 26, (c) Endofill, (d) AH Plus and (e) Biosealer (×100)
Mentions: Figures 1 and 2 present the cellular viability mean values and the comparison of data among the different groups. It could be observed that there was statistically significant difference among the groups (P < 0.05). The cell viability of CG (95.02 ± 1.95) was statistically different from S26 (2.77 ± 2.37), AHP (5.98 ± 5.13) and EF (76.70 ± 7.60). The results in GC were statistically similar - to BS (95.36 ± 1.53). Furthermore, S26 and AHP presented the lowest cellular viability mean values in comparison with the other groups.

Bottom Line: The aim of this study was to evaluate the cytotoxic effect of a new Copaiba oil-based root canal sealer (Biosealer [BS]) on osteoblast-like Osteo-1 cells.S26, EF and AHP presented decreased cellular viability considerably, with statistical significance compared with CG (P < 0.05).The Copaiba oil-based root canal sealer presented promising results in terms of cytotoxicity which indicated its usefulness as a root canal sealer.

View Article: PubMed Central - PubMed

Affiliation: Department of Endodontics, School of Dentistry, Federal University of Amazonas, Manaus, Amazonas, Brazil.

ABSTRACT

Background: The constant development of new root canal sealers has allowed the solution of a large number of clinical cases in endodontics, however, cytotoxicity of such sealers must be tested before their validation as filling materials. The aim of this study was to evaluate the cytotoxic effect of a new Copaiba oil-based root canal sealer (Biosealer [BS]) on osteoblast-like Osteo-1 cells.

Materials and methods: The experimental groups were formed according to the culture medium conditioned with the tested sealers, as follows: Control group (CG) (culture medium without conditioning); Sealer 26 (S26) - culture medium + S26; Endofill (EF) - culture medium + EF; AH Plus (AHP) - culture medium + AHP; and BS - culture medium + BS (Copaiba oil-based sealer). The conditioned culture medium was placed in contact with 2 × 10(4) cells cultivated on 60 mm diameter Petri dishes for 24 h. Then, hemocytometer count was performed to evaluate cellular viability, using Trypan Blue assay. The normal distribution of data was tested by the Kolmogorov-Smirnov test and the values obtained for cellular viability were statistically analyzed (1-way ANOVA, Tukey's test - P < 0.05), with a significance level of 5%.

Results: S26, EF and AHP presented decreased cellular viability considerably, with statistical significance compared with CG (P < 0.05). BS maintained cellular viability similar to CG (P > 0.05).

Conclusion: The Copaiba oil-based root canal sealer presented promising results in terms of cytotoxicity which indicated its usefulness as a root canal sealer.

No MeSH data available.


Related in: MedlinePlus