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Comparison of fluid filtration and bacterial leakage techniques for evaluation of microleakage in endodontics.

Moradi S, Lomee M, Gharechahi M - Dent Res J (Isfahan) (2015 Mar-Apr)

Bottom Line: Many microleakage testing techniques have been introduced through the years, but there has been no agreement as to which technique gives the most accurate results.Mann-Whitney U-test and Chi-square test were used to analyze the data using SPSS (SPSS Inc., Chicago, IL, USA).P less than 0.05 was considered as significant.

View Article: PubMed Central - PubMed

Affiliation: Department of Endodontics, Dental Research Center, School of Dentistry, Mashhad University of Medical Sciences, Mashhad, Iran.

ABSTRACT

Background: Apical leakage assessment is a way to compare the efficiency of a filling material to seal the apical region of the tooth. Many microleakage testing techniques have been introduced through the years, but there has been no agreement as to which technique gives the most accurate results. The aim of this study was to compare the accuracy of fluid filtration and bacterial leakage techniques in the assessment of the apical sealing ability of mineral trioxide aggregate (MTA) and calcium enriched mixture (CEM).

Materials and methods: A sample of 34 extracted single-rooted human teeth were selected and prepared. The samples were divided in to 2 experimental groups. The apical 3 mm of each root was resected at 90° to its long axis and root end preparation was done with ultrasonic tips to a depth of 3 mm and filled with MTA and CEM, respectively. Assessment of apical sealing ability was done with fluid filtration technique and bacterial leakage technique along 90 days with Enterococcus faecalis bacteria. Mann-Whitney U-test and Chi-square test were used to analyze the data using SPSS (SPSS Inc., Chicago, IL, USA). P less than 0.05 was considered as significant.

Results: There was no significant difference in apical sealing ability between MTA and CEM in bacterial leakage and fluid filtration techniques. Samples which had bacterial leakage showed higher leakage values by fluid filtration technique.

Conclusion: Both techniques showed same results and there was no significant difference between fluid filtration and bacterial leakage techniques in assessment of apical microleakage.

No MeSH data available.


Related in: MedlinePlus

(a) The root-micro tubes assembly, (b) the root-micro tubes assembly and test tube
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Figure 3: (a) The root-micro tubes assembly, (b) the root-micro tubes assembly and test tube

Mentions: First, the bottom of an Eppendorf test micro tubes was cut with the blade. The teeth, which all had a layer of hot sticky wax on their coronal half of the root surfaces, were inserted into the cut micro tubes. The coronal part of the roots and the access cavities were in the tubes and the 2-3 mm of apical part was out. The junction between tubes and teeth were sealed internally again by sticky wax. The assemblies [Figure 3a] were sterilized in ethylene oxide gas for 8 h by Anprolene machine (Anderson products Inc., Haw River, NC, USA).


Comparison of fluid filtration and bacterial leakage techniques for evaluation of microleakage in endodontics.

Moradi S, Lomee M, Gharechahi M - Dent Res J (Isfahan) (2015 Mar-Apr)

(a) The root-micro tubes assembly, (b) the root-micro tubes assembly and test tube
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4387621&req=5

Figure 3: (a) The root-micro tubes assembly, (b) the root-micro tubes assembly and test tube
Mentions: First, the bottom of an Eppendorf test micro tubes was cut with the blade. The teeth, which all had a layer of hot sticky wax on their coronal half of the root surfaces, were inserted into the cut micro tubes. The coronal part of the roots and the access cavities were in the tubes and the 2-3 mm of apical part was out. The junction between tubes and teeth were sealed internally again by sticky wax. The assemblies [Figure 3a] were sterilized in ethylene oxide gas for 8 h by Anprolene machine (Anderson products Inc., Haw River, NC, USA).

Bottom Line: Many microleakage testing techniques have been introduced through the years, but there has been no agreement as to which technique gives the most accurate results.Mann-Whitney U-test and Chi-square test were used to analyze the data using SPSS (SPSS Inc., Chicago, IL, USA).P less than 0.05 was considered as significant.

View Article: PubMed Central - PubMed

Affiliation: Department of Endodontics, Dental Research Center, School of Dentistry, Mashhad University of Medical Sciences, Mashhad, Iran.

ABSTRACT

Background: Apical leakage assessment is a way to compare the efficiency of a filling material to seal the apical region of the tooth. Many microleakage testing techniques have been introduced through the years, but there has been no agreement as to which technique gives the most accurate results. The aim of this study was to compare the accuracy of fluid filtration and bacterial leakage techniques in the assessment of the apical sealing ability of mineral trioxide aggregate (MTA) and calcium enriched mixture (CEM).

Materials and methods: A sample of 34 extracted single-rooted human teeth were selected and prepared. The samples were divided in to 2 experimental groups. The apical 3 mm of each root was resected at 90° to its long axis and root end preparation was done with ultrasonic tips to a depth of 3 mm and filled with MTA and CEM, respectively. Assessment of apical sealing ability was done with fluid filtration technique and bacterial leakage technique along 90 days with Enterococcus faecalis bacteria. Mann-Whitney U-test and Chi-square test were used to analyze the data using SPSS (SPSS Inc., Chicago, IL, USA). P less than 0.05 was considered as significant.

Results: There was no significant difference in apical sealing ability between MTA and CEM in bacterial leakage and fluid filtration techniques. Samples which had bacterial leakage showed higher leakage values by fluid filtration technique.

Conclusion: Both techniques showed same results and there was no significant difference between fluid filtration and bacterial leakage techniques in assessment of apical microleakage.

No MeSH data available.


Related in: MedlinePlus