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Localization of hsp27 in the rat submandibular gland following the application of various surgical treatments.

Mizobe K, Kawabe Y, Bando Y, Sakiyama K, Araki H, Amano O - Acta Histochem Cytochem (2014)

Bottom Line: However, no marked changes were observed in the number of Ki67-immunopositive proliferating cells.Therefore, the change in the number of Hsp27-immunopositive cells may have contributed to compensatory hypertrophy.The results of the present study indicate that the expression of Hsp27 in the intercalated duct in the submandibular gland may play a role in the differentiation of acinar cells.

View Article: PubMed Central - PubMed

Affiliation: Division of Anatomy, Department of Human Development and Fostering, Meikai University School of Dentistry , 1-1 Keyakidai, Sakado, Saitama 350-0283, Japan ; Division of Oral Rehabilitation, Department of Restorative and Biomaterials Sciences, Meikai University School of Dentistry.

ABSTRACT
Salivary glands repair and regenerate following various types of injuries and surgical procedures. However, the tissue responses induced in the contralateral glands have yet to be elucidated in detail. Hsp27, a member of the heat-shock protein (Hsp) family, is strongly expressed in physiological environments, particularly during development. Hsp27 was previously shown to play a role in the regulation of acinar cell proliferation and differentiation in the rat submandibular gland. The present study performed the following surgical treatments on the right submandibular glands of adult rats: 1) duct ligation followed by unligation after one week; 2) partial sialoadenectomy; and 3) total sialoadenectomy. Immunohistochemistry for Hsp27 and Ki67 was performed in the experimental and normal contralateral glands, and localization was histologically and morphometrically analyzed. The results obtained revealed the localization of Hsp27 to the intercalated duct in the submandibular glands of non-treated rats. The expression of Hsp27 was strongly induced in both the uninjured contralateral control glands as well as treated glands of experimental rats regardless of the surgical procedure performed. The number of Hsp27-immunopositive cells increased rapidly following surgery, and subsequently returned to the same level as that in non-treated rats after 4 weeks. However, no marked changes were observed in the number of Ki67-immunopositive proliferating cells. Therefore, the change in the number of Hsp27-immunopositive cells may have contributed to compensatory hypertrophy. The results of the present study indicate that the expression of Hsp27 in the intercalated duct in the submandibular gland may play a role in the differentiation of acinar cells.

No MeSH data available.


Related in: MedlinePlus

Photomicrographs of duct-unligated (a, b) and contralateral (c–e) submandibular glands of adult rats stained with H-E (a, c) or anti-Hsp27 antibody (b, d, e) at 3 days (c, d) or 2 weeks (a, b, e) after unligation surgery. Hsp27-immunopositive cells (arrows) were located in the intercalated duct of operated glands, which represented regenerating acini, 2 weeks after surgery (b). In contralateral glands, Hsp27-immunopositive cells were located in the intercalated duct (arrowheads) from the distal to proximal end 3 days (c, d) and 2 weeks (e) after unligation. Acini (asterisks) are immunonegative for Hsp27. Bars=500 μm.
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Figure 3: Photomicrographs of duct-unligated (a, b) and contralateral (c–e) submandibular glands of adult rats stained with H-E (a, c) or anti-Hsp27 antibody (b, d, e) at 3 days (c, d) or 2 weeks (a, b, e) after unligation surgery. Hsp27-immunopositive cells (arrows) were located in the intercalated duct of operated glands, which represented regenerating acini, 2 weeks after surgery (b). In contralateral glands, Hsp27-immunopositive cells were located in the intercalated duct (arrowheads) from the distal to proximal end 3 days (c, d) and 2 weeks (e) after unligation. Acini (asterisks) are immunonegative for Hsp27. Bars=500 μm.

Mentions: Epithelial buds arose from the duct-like structures in operated (right) submandibular glands 3 days after unligation, and the localization of Hsp27-immunopositive cells were detected in epithelial bulging at the distal end of duct-like structures (not shown), as has been reported previously by Takahashi-Horiuchi et al. [27]. The architecture of the tissue was normal 2 weeks after surgery. However, it was slightly smaller than that on the contralateral side, and a duct system that included the ID and acini was observed (Fig. 3a). Immunohistochemistry for Hsp27 showed the localization of small, round Hsp27-positive cells in the ID (Fig. 3b).


Localization of hsp27 in the rat submandibular gland following the application of various surgical treatments.

Mizobe K, Kawabe Y, Bando Y, Sakiyama K, Araki H, Amano O - Acta Histochem Cytochem (2014)

Photomicrographs of duct-unligated (a, b) and contralateral (c–e) submandibular glands of adult rats stained with H-E (a, c) or anti-Hsp27 antibody (b, d, e) at 3 days (c, d) or 2 weeks (a, b, e) after unligation surgery. Hsp27-immunopositive cells (arrows) were located in the intercalated duct of operated glands, which represented regenerating acini, 2 weeks after surgery (b). In contralateral glands, Hsp27-immunopositive cells were located in the intercalated duct (arrowheads) from the distal to proximal end 3 days (c, d) and 2 weeks (e) after unligation. Acini (asterisks) are immunonegative for Hsp27. Bars=500 μm.
© Copyright Policy - open-access
Related In: Results  -  Collection

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Figure 3: Photomicrographs of duct-unligated (a, b) and contralateral (c–e) submandibular glands of adult rats stained with H-E (a, c) or anti-Hsp27 antibody (b, d, e) at 3 days (c, d) or 2 weeks (a, b, e) after unligation surgery. Hsp27-immunopositive cells (arrows) were located in the intercalated duct of operated glands, which represented regenerating acini, 2 weeks after surgery (b). In contralateral glands, Hsp27-immunopositive cells were located in the intercalated duct (arrowheads) from the distal to proximal end 3 days (c, d) and 2 weeks (e) after unligation. Acini (asterisks) are immunonegative for Hsp27. Bars=500 μm.
Mentions: Epithelial buds arose from the duct-like structures in operated (right) submandibular glands 3 days after unligation, and the localization of Hsp27-immunopositive cells were detected in epithelial bulging at the distal end of duct-like structures (not shown), as has been reported previously by Takahashi-Horiuchi et al. [27]. The architecture of the tissue was normal 2 weeks after surgery. However, it was slightly smaller than that on the contralateral side, and a duct system that included the ID and acini was observed (Fig. 3a). Immunohistochemistry for Hsp27 showed the localization of small, round Hsp27-positive cells in the ID (Fig. 3b).

Bottom Line: However, no marked changes were observed in the number of Ki67-immunopositive proliferating cells.Therefore, the change in the number of Hsp27-immunopositive cells may have contributed to compensatory hypertrophy.The results of the present study indicate that the expression of Hsp27 in the intercalated duct in the submandibular gland may play a role in the differentiation of acinar cells.

View Article: PubMed Central - PubMed

Affiliation: Division of Anatomy, Department of Human Development and Fostering, Meikai University School of Dentistry , 1-1 Keyakidai, Sakado, Saitama 350-0283, Japan ; Division of Oral Rehabilitation, Department of Restorative and Biomaterials Sciences, Meikai University School of Dentistry.

ABSTRACT
Salivary glands repair and regenerate following various types of injuries and surgical procedures. However, the tissue responses induced in the contralateral glands have yet to be elucidated in detail. Hsp27, a member of the heat-shock protein (Hsp) family, is strongly expressed in physiological environments, particularly during development. Hsp27 was previously shown to play a role in the regulation of acinar cell proliferation and differentiation in the rat submandibular gland. The present study performed the following surgical treatments on the right submandibular glands of adult rats: 1) duct ligation followed by unligation after one week; 2) partial sialoadenectomy; and 3) total sialoadenectomy. Immunohistochemistry for Hsp27 and Ki67 was performed in the experimental and normal contralateral glands, and localization was histologically and morphometrically analyzed. The results obtained revealed the localization of Hsp27 to the intercalated duct in the submandibular glands of non-treated rats. The expression of Hsp27 was strongly induced in both the uninjured contralateral control glands as well as treated glands of experimental rats regardless of the surgical procedure performed. The number of Hsp27-immunopositive cells increased rapidly following surgery, and subsequently returned to the same level as that in non-treated rats after 4 weeks. However, no marked changes were observed in the number of Ki67-immunopositive proliferating cells. Therefore, the change in the number of Hsp27-immunopositive cells may have contributed to compensatory hypertrophy. The results of the present study indicate that the expression of Hsp27 in the intercalated duct in the submandibular gland may play a role in the differentiation of acinar cells.

No MeSH data available.


Related in: MedlinePlus