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Immunohistochemical Analysis of Collagen IV and Laminin Expression in Spontaneous Melanoma Regression in the Melanoma-Bearing Libechov Minipig.

Planska D, Burocziova M, Strnadel J, Horak V - Acta Histochem Cytochem (2015)

Bottom Line: Its detailed study cannot be performed in patients due to ethical reasons.Collagen IV expression slightly increased in tumours of 10-week-old pigs showing extracellular fibrous appearance.We considered the age of 10 weeks as a turning point in the transition between tumour growth and SR of the MeLiM melanoma.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Tumor Biology, Institute of Animal Physiology and Genetics AS CR , v.v.i, Libechov, Czech Republic ; Faculty of Science, Charles University , Prague, Czech Republic ; Department of Immunology, Third Faculty of Medicine, Charles University , Prague, Czech Republic.

ABSTRACT
Spontaneous regression (SR) of human melanoma is a rare, well-documented phenomenon that is not still fully understood. Its detailed study cannot be performed in patients due to ethical reasons. Using the Melanoma-bearing Libechov Minipig (MeLiM) animals of various ages (from 3 weeks to 8 months) we implemented a long-term monitoring of melanoma growth and SR. We focused on immunohistochemical detection of two important extracellular matrix proteins, collagen IV and laminin, which are associated with cancer. We showed that SR of melanoma is a highly dynamic process. The expression of collagen IV and laminin correlated with changes in population of melanoma cells. Tumours of 3-week-old animals consisted primarily of melanoma cells with a granular expression of collagen IV and laminin around them. Thereafter, melanoma cells were gradually destroyed and tumour tissue was rebuilt into the connective tissue. Collagen IV expression slightly increased in tumours of 10-week-old pigs showing extracellular fibrous appearance. In tumours of older animals, areas lacking melanoma cells demonstrated a low expression and areas still containing melanoma cells a high expression of both proteins. We considered the age of 10 weeks as a turning point in the transition between tumour growth and SR of the MeLiM melanoma.

No MeSH data available.


Related in: MedlinePlus

Haematoxylin-eosin staining of swine melanoma cryosections. (A) 3-week-old pig. (B) 10-week-old pig. (C) 3-month-old pig. (D) 8-month-old pig. Bar=50 μm.
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Figure 4: Haematoxylin-eosin staining of swine melanoma cryosections. (A) 3-week-old pig. (B) 10-week-old pig. (C) 3-month-old pig. (D) 8-month-old pig. Bar=50 μm.

Mentions: Melanomas from 3-week-old pigs were almost exclusively comprised of very uniform population of smaller melanoma cells with granular brown-black melanin (Fig. 4A). The clear moderate accumulation of granular collagen IV was observed in the extracellular spaces among the melanoma cells (Table 2A; Fig. 3A). We found very similar collagen IV staining pattern in the melanomas excised from 4-week-old pigs. However, the tumour morphology was slightly changed. In addition to the melanoma cell type observed in the tumours of 3-week-old pigs, a new population of larger melanoma cells with a dense dark brown melanin was observed. In the tumours of 6-week-old pigs, a change from granular to slightly fibrous staining pattern of collagen IV was detected. The protein was found around melanoma cells and its expression was generally reduced. The size of both melanoma cell types increased and short strands of non-pigmented tumour stroma passed through melanoma tissue. The expression of collagen IV further decreased to very low levels in the tumours of 8-week-old pigs. It filled some spaces among melanoma cells. The size of melanoma cells generally increased. However, some of them were destroyed and small particles of melanin were sparsely scattered among the surrounding cells.


Immunohistochemical Analysis of Collagen IV and Laminin Expression in Spontaneous Melanoma Regression in the Melanoma-Bearing Libechov Minipig.

Planska D, Burocziova M, Strnadel J, Horak V - Acta Histochem Cytochem (2015)

Haematoxylin-eosin staining of swine melanoma cryosections. (A) 3-week-old pig. (B) 10-week-old pig. (C) 3-month-old pig. (D) 8-month-old pig. Bar=50 μm.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4387259&req=5

Figure 4: Haematoxylin-eosin staining of swine melanoma cryosections. (A) 3-week-old pig. (B) 10-week-old pig. (C) 3-month-old pig. (D) 8-month-old pig. Bar=50 μm.
Mentions: Melanomas from 3-week-old pigs were almost exclusively comprised of very uniform population of smaller melanoma cells with granular brown-black melanin (Fig. 4A). The clear moderate accumulation of granular collagen IV was observed in the extracellular spaces among the melanoma cells (Table 2A; Fig. 3A). We found very similar collagen IV staining pattern in the melanomas excised from 4-week-old pigs. However, the tumour morphology was slightly changed. In addition to the melanoma cell type observed in the tumours of 3-week-old pigs, a new population of larger melanoma cells with a dense dark brown melanin was observed. In the tumours of 6-week-old pigs, a change from granular to slightly fibrous staining pattern of collagen IV was detected. The protein was found around melanoma cells and its expression was generally reduced. The size of both melanoma cell types increased and short strands of non-pigmented tumour stroma passed through melanoma tissue. The expression of collagen IV further decreased to very low levels in the tumours of 8-week-old pigs. It filled some spaces among melanoma cells. The size of melanoma cells generally increased. However, some of them were destroyed and small particles of melanin were sparsely scattered among the surrounding cells.

Bottom Line: Its detailed study cannot be performed in patients due to ethical reasons.Collagen IV expression slightly increased in tumours of 10-week-old pigs showing extracellular fibrous appearance.We considered the age of 10 weeks as a turning point in the transition between tumour growth and SR of the MeLiM melanoma.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Tumor Biology, Institute of Animal Physiology and Genetics AS CR , v.v.i, Libechov, Czech Republic ; Faculty of Science, Charles University , Prague, Czech Republic ; Department of Immunology, Third Faculty of Medicine, Charles University , Prague, Czech Republic.

ABSTRACT
Spontaneous regression (SR) of human melanoma is a rare, well-documented phenomenon that is not still fully understood. Its detailed study cannot be performed in patients due to ethical reasons. Using the Melanoma-bearing Libechov Minipig (MeLiM) animals of various ages (from 3 weeks to 8 months) we implemented a long-term monitoring of melanoma growth and SR. We focused on immunohistochemical detection of two important extracellular matrix proteins, collagen IV and laminin, which are associated with cancer. We showed that SR of melanoma is a highly dynamic process. The expression of collagen IV and laminin correlated with changes in population of melanoma cells. Tumours of 3-week-old animals consisted primarily of melanoma cells with a granular expression of collagen IV and laminin around them. Thereafter, melanoma cells were gradually destroyed and tumour tissue was rebuilt into the connective tissue. Collagen IV expression slightly increased in tumours of 10-week-old pigs showing extracellular fibrous appearance. In tumours of older animals, areas lacking melanoma cells demonstrated a low expression and areas still containing melanoma cells a high expression of both proteins. We considered the age of 10 weeks as a turning point in the transition between tumour growth and SR of the MeLiM melanoma.

No MeSH data available.


Related in: MedlinePlus