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Re-epithelialization of the Buccal Mucosa after Alkaline Chemical Injury.

Takaichi S, Muramatsu T, Lee JM, Jung HS, Shinozaki N, Katakura A, Yamane GY - Acta Histochem Cytochem (2014)

Bottom Line: However, the effects of an alkaline pH on the oral mucosa had not been elucidated.Cytokeratin 14-positive and PCNA-positive basal keratinocytes, indicating proliferation, were localized over the entire layer of the epithelium at the wound margin.These results imply that basal keratinocytes at the wound margin migrate to the wound surface, provoke differentiation and keratinization during epithelial outgrowth and that epithelial cells are supplied from the wound margin to the epithelial outgrowth after alkaline chemical injury.

View Article: PubMed Central - PubMed

Affiliation: Oral Health Science Center hrc7, Tokyo Dental College , Japan ; Department of Oral Medicine, Oral and Maxillofacial Surgery, Tokyo Dental College , Japan.

ABSTRACT
Alkaline conditions in the oral cavity may be caused by a variety of stimuli, including tobacco products, antacids, alkaline drinking water and bicarbonate toothpaste. However, the effects of an alkaline pH on the oral mucosa had not been elucidated. The purpose of this study was to investigate how basal keratinocytes are actively involved in re-epithelialization after alkaline chemical injury. We generated epithelial defects in the oral mucosa of mice by applying an alkaline chemical, and the localization of cytokeratin 13, cytokeratin 14, PCNA and p63 was investigated during the re-epithelialization process. PCNA- and p63-positive staining was seen in basal cells covering the wound surface at 1 day after the chemical injury. Cytokeratin 14-positive and PCNA-negative basal keratinocytes were localized in a few layers of the wound epithelium during epithelial outgrowth. Cytokeratin 14-positive and PCNA-positive basal keratinocytes, indicating proliferation, were localized over the entire layer of the epithelium at the wound margin. These results imply that basal keratinocytes at the wound margin migrate to the wound surface, provoke differentiation and keratinization during epithelial outgrowth and that epithelial cells are supplied from the wound margin to the epithelial outgrowth after alkaline chemical injury.

No MeSH data available.


Related in: MedlinePlus

Histological observations of chemically injured oral mucosa during epithelial outgrowth after 24 hr (A, A’), 72 hr (B, B’), 120 hr (C, C’) and 1 week (D, D’) after injury and in normal tissue (Control; E, E’). Higher magnifications of the boxed regions in A–E are shown as A’–E’: respectively. The epithelium of the buccal mucosa was identified as the stratified squamous type. E, E’: Epithelial elongation is observed from both sides of the wound margin toward the chemical injury. A, A’, B, B’: Re-epithelialization is observed in the degenerated muscle tissue. C, C’: Regenerated epithelia from both sides of the wound margin are connected. D, D’: The epithelium of the buccal mucosa has been completely regenerated and resembles the normal epithelium (E, E’). Bars=100 μm (A–E), 150 μm (A’–E’).
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Figure 1: Histological observations of chemically injured oral mucosa during epithelial outgrowth after 24 hr (A, A’), 72 hr (B, B’), 120 hr (C, C’) and 1 week (D, D’) after injury and in normal tissue (Control; E, E’). Higher magnifications of the boxed regions in A–E are shown as A’–E’: respectively. The epithelium of the buccal mucosa was identified as the stratified squamous type. E, E’: Epithelial elongation is observed from both sides of the wound margin toward the chemical injury. A, A’, B, B’: Re-epithelialization is observed in the degenerated muscle tissue. C, C’: Regenerated epithelia from both sides of the wound margin are connected. D, D’: The epithelium of the buccal mucosa has been completely regenerated and resembles the normal epithelium (E, E’). Bars=100 μm (A–E), 150 μm (A’–E’).

Mentions: We observed two areas, the wound margin and the outgrowth, during the wound healing process, as shown in Figure 1.


Re-epithelialization of the Buccal Mucosa after Alkaline Chemical Injury.

Takaichi S, Muramatsu T, Lee JM, Jung HS, Shinozaki N, Katakura A, Yamane GY - Acta Histochem Cytochem (2014)

Histological observations of chemically injured oral mucosa during epithelial outgrowth after 24 hr (A, A’), 72 hr (B, B’), 120 hr (C, C’) and 1 week (D, D’) after injury and in normal tissue (Control; E, E’). Higher magnifications of the boxed regions in A–E are shown as A’–E’: respectively. The epithelium of the buccal mucosa was identified as the stratified squamous type. E, E’: Epithelial elongation is observed from both sides of the wound margin toward the chemical injury. A, A’, B, B’: Re-epithelialization is observed in the degenerated muscle tissue. C, C’: Regenerated epithelia from both sides of the wound margin are connected. D, D’: The epithelium of the buccal mucosa has been completely regenerated and resembles the normal epithelium (E, E’). Bars=100 μm (A–E), 150 μm (A’–E’).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4387240&req=5

Figure 1: Histological observations of chemically injured oral mucosa during epithelial outgrowth after 24 hr (A, A’), 72 hr (B, B’), 120 hr (C, C’) and 1 week (D, D’) after injury and in normal tissue (Control; E, E’). Higher magnifications of the boxed regions in A–E are shown as A’–E’: respectively. The epithelium of the buccal mucosa was identified as the stratified squamous type. E, E’: Epithelial elongation is observed from both sides of the wound margin toward the chemical injury. A, A’, B, B’: Re-epithelialization is observed in the degenerated muscle tissue. C, C’: Regenerated epithelia from both sides of the wound margin are connected. D, D’: The epithelium of the buccal mucosa has been completely regenerated and resembles the normal epithelium (E, E’). Bars=100 μm (A–E), 150 μm (A’–E’).
Mentions: We observed two areas, the wound margin and the outgrowth, during the wound healing process, as shown in Figure 1.

Bottom Line: However, the effects of an alkaline pH on the oral mucosa had not been elucidated.Cytokeratin 14-positive and PCNA-positive basal keratinocytes, indicating proliferation, were localized over the entire layer of the epithelium at the wound margin.These results imply that basal keratinocytes at the wound margin migrate to the wound surface, provoke differentiation and keratinization during epithelial outgrowth and that epithelial cells are supplied from the wound margin to the epithelial outgrowth after alkaline chemical injury.

View Article: PubMed Central - PubMed

Affiliation: Oral Health Science Center hrc7, Tokyo Dental College , Japan ; Department of Oral Medicine, Oral and Maxillofacial Surgery, Tokyo Dental College , Japan.

ABSTRACT
Alkaline conditions in the oral cavity may be caused by a variety of stimuli, including tobacco products, antacids, alkaline drinking water and bicarbonate toothpaste. However, the effects of an alkaline pH on the oral mucosa had not been elucidated. The purpose of this study was to investigate how basal keratinocytes are actively involved in re-epithelialization after alkaline chemical injury. We generated epithelial defects in the oral mucosa of mice by applying an alkaline chemical, and the localization of cytokeratin 13, cytokeratin 14, PCNA and p63 was investigated during the re-epithelialization process. PCNA- and p63-positive staining was seen in basal cells covering the wound surface at 1 day after the chemical injury. Cytokeratin 14-positive and PCNA-negative basal keratinocytes were localized in a few layers of the wound epithelium during epithelial outgrowth. Cytokeratin 14-positive and PCNA-positive basal keratinocytes, indicating proliferation, were localized over the entire layer of the epithelium at the wound margin. These results imply that basal keratinocytes at the wound margin migrate to the wound surface, provoke differentiation and keratinization during epithelial outgrowth and that epithelial cells are supplied from the wound margin to the epithelial outgrowth after alkaline chemical injury.

No MeSH data available.


Related in: MedlinePlus