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Effect of Nigella sativa fixed oil on ethanol toxicity in rats.

Pourbakhsh H, Taghiabadi E, Abnous K, Hariri AT, Hosseini SM, Hosseinzadeh H - Iran J Basic Med Sci (2014)

Bottom Line: Moreover, NSO improved the level of serum liver enzymes (including alanine transaminase (ALT), aspartate transaminase (AST), alkaline phosphatase (ALP) and glutathione (GSH) content in liver and kidney tissues in ethanol-treated rats.Western blot analysis and quantitative real time RT-PCR showed that NSO treatment inhibited apoptosis stimulated by ethanol through decreasing the Bax/Bcl-2 ratio (both protein and mRNA levels), cleaved caspase-3, cleaved caspase-8 and cleaved caspase-9 level in liver and kidney.This study showed that NSO may have protective effects against hepatotoxicity and renal toxicity of ethanol by decreasing lipid peroxidation and inflammation and preventing apoptosis.

View Article: PubMed Central - PubMed

Affiliation: Food Control Laboratory, Food and Drug Administration, Shiraz University of Medical Sciences, Shiraz, Iran.

ABSTRACT

Objectives: This study was planned to appraise the protective effect of Nigella sativa fixed oil (NSO) against subchronic ethanol induced toxicity in rats.

Materials and methods: Studies were carried out on six groups of six animals each, including control (normal saline, gavage), ethanol (3 g/kg/day, gavage), NSO (0.125, 0.25 and 0.5 ml/Kg/day, IP) plus ethanol and NSO (0.5 ml/Kg/day, IP) groups. Treatments were continued for 4 weeks.

Results: According to data, treatment with NSO attenuated ethanol-induced increased levels of malondialdehyde (MDA), tumor necrosis factor α (TNF-α) and interleukin-6 (IL-6), as well as histopathological changes in liver and kidney tissues. Moreover, NSO improved the level of serum liver enzymes (including alanine transaminase (ALT), aspartate transaminase (AST), alkaline phosphatase (ALP) and glutathione (GSH) content in liver and kidney tissues in ethanol-treated rats. Western blot analysis and quantitative real time RT-PCR showed that NSO treatment inhibited apoptosis stimulated by ethanol through decreasing the Bax/Bcl-2 ratio (both protein and mRNA levels), cleaved caspase-3, cleaved caspase-8 and cleaved caspase-9 level in liver and kidney.

Conclusion: This study showed that NSO may have protective effects against hepatotoxicity and renal toxicity of ethanol by decreasing lipid peroxidation and inflammation and preventing apoptosis.

No MeSH data available.


Related in: MedlinePlus

Effect of NSO (0.5 ml/kg) and ethanol on the protein level of caspase-3 (cleaved caspase-3) in the rat kidney tissue. (A) Representative Western blots showing specific bands for cleaved caspase-3 (17 KDa) and β-actin as an internal control. Equal amounts of protein sample (50 μg) obtained from whole kidney homogenate were applied in each lane. (B) Densitometric data of protein analysis. Data are expressed as the mean ± SEM. ***P<0.001 vs control group, ###P<0.001 vs ethanol group
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Figure 10: Effect of NSO (0.5 ml/kg) and ethanol on the protein level of caspase-3 (cleaved caspase-3) in the rat kidney tissue. (A) Representative Western blots showing specific bands for cleaved caspase-3 (17 KDa) and β-actin as an internal control. Equal amounts of protein sample (50 μg) obtained from whole kidney homogenate were applied in each lane. (B) Densitometric data of protein analysis. Data are expressed as the mean ± SEM. ***P<0.001 vs control group, ###P<0.001 vs ethanol group

Mentions: Western blot analysis showed that protein expression of Bax/Bcl2 was up-regulated in ethanol group in the liver and kidney (P<0.001 and P<0.01, respectively) (Figures 7 and 8). Also protein level of caspase-3, caspase-8 and caspase-9 were up-regulated by ethanol in the liver (Figures 9, 11 and 13) and kidney (Figures 10, 12 and 14) tissues. Co-treatment of ethanol with NSO (0.5 ml/kg) significantly decreased the ratio of Bax/Bcl2 in liver and kidney (P<0.001 and P<0.05, respectively) (Figures 7 and 8). Furthermore, NSO (0.5 ml/kg) inhibited the ethanol-induced activation of caspase-3 (19 KDa) and attenuated the protein level of cleaved caspase-3 (17 KDa) in the liver and kidney (P<0.01 and P<0.001, respectively) (Figure 9 and 10). Administration of NSO to animals significantly decreased the liver and kidney ratio of cleaved caspase-8 (P<0.001) (Figures 11 and 12) and the liver ratio of cleaved caspase-9 (P<0.01) (Figure 13). Administration of ethanol with NSO indicated no significant change in the level of cleaved caspase-9 compared to the ethanol group in the kidney tissue (P>0.05) (Figure 14).


Effect of Nigella sativa fixed oil on ethanol toxicity in rats.

Pourbakhsh H, Taghiabadi E, Abnous K, Hariri AT, Hosseini SM, Hosseinzadeh H - Iran J Basic Med Sci (2014)

Effect of NSO (0.5 ml/kg) and ethanol on the protein level of caspase-3 (cleaved caspase-3) in the rat kidney tissue. (A) Representative Western blots showing specific bands for cleaved caspase-3 (17 KDa) and β-actin as an internal control. Equal amounts of protein sample (50 μg) obtained from whole kidney homogenate were applied in each lane. (B) Densitometric data of protein analysis. Data are expressed as the mean ± SEM. ***P<0.001 vs control group, ###P<0.001 vs ethanol group
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4387225&req=5

Figure 10: Effect of NSO (0.5 ml/kg) and ethanol on the protein level of caspase-3 (cleaved caspase-3) in the rat kidney tissue. (A) Representative Western blots showing specific bands for cleaved caspase-3 (17 KDa) and β-actin as an internal control. Equal amounts of protein sample (50 μg) obtained from whole kidney homogenate were applied in each lane. (B) Densitometric data of protein analysis. Data are expressed as the mean ± SEM. ***P<0.001 vs control group, ###P<0.001 vs ethanol group
Mentions: Western blot analysis showed that protein expression of Bax/Bcl2 was up-regulated in ethanol group in the liver and kidney (P<0.001 and P<0.01, respectively) (Figures 7 and 8). Also protein level of caspase-3, caspase-8 and caspase-9 were up-regulated by ethanol in the liver (Figures 9, 11 and 13) and kidney (Figures 10, 12 and 14) tissues. Co-treatment of ethanol with NSO (0.5 ml/kg) significantly decreased the ratio of Bax/Bcl2 in liver and kidney (P<0.001 and P<0.05, respectively) (Figures 7 and 8). Furthermore, NSO (0.5 ml/kg) inhibited the ethanol-induced activation of caspase-3 (19 KDa) and attenuated the protein level of cleaved caspase-3 (17 KDa) in the liver and kidney (P<0.01 and P<0.001, respectively) (Figure 9 and 10). Administration of NSO to animals significantly decreased the liver and kidney ratio of cleaved caspase-8 (P<0.001) (Figures 11 and 12) and the liver ratio of cleaved caspase-9 (P<0.01) (Figure 13). Administration of ethanol with NSO indicated no significant change in the level of cleaved caspase-9 compared to the ethanol group in the kidney tissue (P>0.05) (Figure 14).

Bottom Line: Moreover, NSO improved the level of serum liver enzymes (including alanine transaminase (ALT), aspartate transaminase (AST), alkaline phosphatase (ALP) and glutathione (GSH) content in liver and kidney tissues in ethanol-treated rats.Western blot analysis and quantitative real time RT-PCR showed that NSO treatment inhibited apoptosis stimulated by ethanol through decreasing the Bax/Bcl-2 ratio (both protein and mRNA levels), cleaved caspase-3, cleaved caspase-8 and cleaved caspase-9 level in liver and kidney.This study showed that NSO may have protective effects against hepatotoxicity and renal toxicity of ethanol by decreasing lipid peroxidation and inflammation and preventing apoptosis.

View Article: PubMed Central - PubMed

Affiliation: Food Control Laboratory, Food and Drug Administration, Shiraz University of Medical Sciences, Shiraz, Iran.

ABSTRACT

Objectives: This study was planned to appraise the protective effect of Nigella sativa fixed oil (NSO) against subchronic ethanol induced toxicity in rats.

Materials and methods: Studies were carried out on six groups of six animals each, including control (normal saline, gavage), ethanol (3 g/kg/day, gavage), NSO (0.125, 0.25 and 0.5 ml/Kg/day, IP) plus ethanol and NSO (0.5 ml/Kg/day, IP) groups. Treatments were continued for 4 weeks.

Results: According to data, treatment with NSO attenuated ethanol-induced increased levels of malondialdehyde (MDA), tumor necrosis factor α (TNF-α) and interleukin-6 (IL-6), as well as histopathological changes in liver and kidney tissues. Moreover, NSO improved the level of serum liver enzymes (including alanine transaminase (ALT), aspartate transaminase (AST), alkaline phosphatase (ALP) and glutathione (GSH) content in liver and kidney tissues in ethanol-treated rats. Western blot analysis and quantitative real time RT-PCR showed that NSO treatment inhibited apoptosis stimulated by ethanol through decreasing the Bax/Bcl-2 ratio (both protein and mRNA levels), cleaved caspase-3, cleaved caspase-8 and cleaved caspase-9 level in liver and kidney.

Conclusion: This study showed that NSO may have protective effects against hepatotoxicity and renal toxicity of ethanol by decreasing lipid peroxidation and inflammation and preventing apoptosis.

No MeSH data available.


Related in: MedlinePlus