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Effect of Nigella sativa fixed oil on ethanol toxicity in rats.

Pourbakhsh H, Taghiabadi E, Abnous K, Hariri AT, Hosseini SM, Hosseinzadeh H - Iran J Basic Med Sci (2014)

Bottom Line: Moreover, NSO improved the level of serum liver enzymes (including alanine transaminase (ALT), aspartate transaminase (AST), alkaline phosphatase (ALP) and glutathione (GSH) content in liver and kidney tissues in ethanol-treated rats.Western blot analysis and quantitative real time RT-PCR showed that NSO treatment inhibited apoptosis stimulated by ethanol through decreasing the Bax/Bcl-2 ratio (both protein and mRNA levels), cleaved caspase-3, cleaved caspase-8 and cleaved caspase-9 level in liver and kidney.This study showed that NSO may have protective effects against hepatotoxicity and renal toxicity of ethanol by decreasing lipid peroxidation and inflammation and preventing apoptosis.

View Article: PubMed Central - PubMed

Affiliation: Food Control Laboratory, Food and Drug Administration, Shiraz University of Medical Sciences, Shiraz, Iran.

ABSTRACT

Objectives: This study was planned to appraise the protective effect of Nigella sativa fixed oil (NSO) against subchronic ethanol induced toxicity in rats.

Materials and methods: Studies were carried out on six groups of six animals each, including control (normal saline, gavage), ethanol (3 g/kg/day, gavage), NSO (0.125, 0.25 and 0.5 ml/Kg/day, IP) plus ethanol and NSO (0.5 ml/Kg/day, IP) groups. Treatments were continued for 4 weeks.

Results: According to data, treatment with NSO attenuated ethanol-induced increased levels of malondialdehyde (MDA), tumor necrosis factor α (TNF-α) and interleukin-6 (IL-6), as well as histopathological changes in liver and kidney tissues. Moreover, NSO improved the level of serum liver enzymes (including alanine transaminase (ALT), aspartate transaminase (AST), alkaline phosphatase (ALP) and glutathione (GSH) content in liver and kidney tissues in ethanol-treated rats. Western blot analysis and quantitative real time RT-PCR showed that NSO treatment inhibited apoptosis stimulated by ethanol through decreasing the Bax/Bcl-2 ratio (both protein and mRNA levels), cleaved caspase-3, cleaved caspase-8 and cleaved caspase-9 level in liver and kidney.

Conclusion: This study showed that NSO may have protective effects against hepatotoxicity and renal toxicity of ethanol by decreasing lipid peroxidation and inflammation and preventing apoptosis.

No MeSH data available.


Related in: MedlinePlus

Effects of ethanol and Nigella sativa fixed oil (NSO) treatment (4 weeks) on serum levels of AST (A), ALT (B) and ALP (C) in rats. NSO was administered intraperitoneally, once a day. Ethanol was administered via gavage to rats once a day. Data showed as mean ± SEM, *Comparison with control, #Comparison with ethanol-treated group. * or # P<0.05, ##P<0.01 and *** or ### P<0.001, Tukey-Kramer test, n=6
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Figure 1: Effects of ethanol and Nigella sativa fixed oil (NSO) treatment (4 weeks) on serum levels of AST (A), ALT (B) and ALP (C) in rats. NSO was administered intraperitoneally, once a day. Ethanol was administered via gavage to rats once a day. Data showed as mean ± SEM, *Comparison with control, #Comparison with ethanol-treated group. * or # P<0.05, ##P<0.01 and *** or ### P<0.001, Tukey-Kramer test, n=6

Mentions: AST, ALT and ALP were increased significantly in the ethanol-treated group when compared to the control group. NSO prevented this effect of ethanol on AST, ALT and ALP levels (Figure 1).


Effect of Nigella sativa fixed oil on ethanol toxicity in rats.

Pourbakhsh H, Taghiabadi E, Abnous K, Hariri AT, Hosseini SM, Hosseinzadeh H - Iran J Basic Med Sci (2014)

Effects of ethanol and Nigella sativa fixed oil (NSO) treatment (4 weeks) on serum levels of AST (A), ALT (B) and ALP (C) in rats. NSO was administered intraperitoneally, once a day. Ethanol was administered via gavage to rats once a day. Data showed as mean ± SEM, *Comparison with control, #Comparison with ethanol-treated group. * or # P<0.05, ##P<0.01 and *** or ### P<0.001, Tukey-Kramer test, n=6
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4387225&req=5

Figure 1: Effects of ethanol and Nigella sativa fixed oil (NSO) treatment (4 weeks) on serum levels of AST (A), ALT (B) and ALP (C) in rats. NSO was administered intraperitoneally, once a day. Ethanol was administered via gavage to rats once a day. Data showed as mean ± SEM, *Comparison with control, #Comparison with ethanol-treated group. * or # P<0.05, ##P<0.01 and *** or ### P<0.001, Tukey-Kramer test, n=6
Mentions: AST, ALT and ALP were increased significantly in the ethanol-treated group when compared to the control group. NSO prevented this effect of ethanol on AST, ALT and ALP levels (Figure 1).

Bottom Line: Moreover, NSO improved the level of serum liver enzymes (including alanine transaminase (ALT), aspartate transaminase (AST), alkaline phosphatase (ALP) and glutathione (GSH) content in liver and kidney tissues in ethanol-treated rats.Western blot analysis and quantitative real time RT-PCR showed that NSO treatment inhibited apoptosis stimulated by ethanol through decreasing the Bax/Bcl-2 ratio (both protein and mRNA levels), cleaved caspase-3, cleaved caspase-8 and cleaved caspase-9 level in liver and kidney.This study showed that NSO may have protective effects against hepatotoxicity and renal toxicity of ethanol by decreasing lipid peroxidation and inflammation and preventing apoptosis.

View Article: PubMed Central - PubMed

Affiliation: Food Control Laboratory, Food and Drug Administration, Shiraz University of Medical Sciences, Shiraz, Iran.

ABSTRACT

Objectives: This study was planned to appraise the protective effect of Nigella sativa fixed oil (NSO) against subchronic ethanol induced toxicity in rats.

Materials and methods: Studies were carried out on six groups of six animals each, including control (normal saline, gavage), ethanol (3 g/kg/day, gavage), NSO (0.125, 0.25 and 0.5 ml/Kg/day, IP) plus ethanol and NSO (0.5 ml/Kg/day, IP) groups. Treatments were continued for 4 weeks.

Results: According to data, treatment with NSO attenuated ethanol-induced increased levels of malondialdehyde (MDA), tumor necrosis factor α (TNF-α) and interleukin-6 (IL-6), as well as histopathological changes in liver and kidney tissues. Moreover, NSO improved the level of serum liver enzymes (including alanine transaminase (ALT), aspartate transaminase (AST), alkaline phosphatase (ALP) and glutathione (GSH) content in liver and kidney tissues in ethanol-treated rats. Western blot analysis and quantitative real time RT-PCR showed that NSO treatment inhibited apoptosis stimulated by ethanol through decreasing the Bax/Bcl-2 ratio (both protein and mRNA levels), cleaved caspase-3, cleaved caspase-8 and cleaved caspase-9 level in liver and kidney.

Conclusion: This study showed that NSO may have protective effects against hepatotoxicity and renal toxicity of ethanol by decreasing lipid peroxidation and inflammation and preventing apoptosis.

No MeSH data available.


Related in: MedlinePlus