Limits...
Inhibitors of eicosanoid biosynthesis influencing the transcripts level of sHSP21.4 gene induced by pathogen infections, in Antheraea pernyi.

Zhang C, Dai L, Wang L, Qian C, Wei G, Li J, Zhu B, Liu C - PLoS ONE (2015)

Bottom Line: Meanwhile, knock down of Ap-sHSP21.4 with dsRNA result in the decrease at the expression levels of several immune response-related genes (defensin, Dopa decarboxylase, Toll1, lysozyme and Kazal-type serine protease inhibitor).Additionally, the impact of eicosanoid biosynthesis on the expression of Ap-sHSP21.4 response to NPV was determined using qPCR, inhibitors of eicosanoid biosynthesis significantly suppress Ap-HSP21.4 expression upon NPV challenge.These results will shed light in the understanding of the pathogen-host interaction in A. pernyi.

View Article: PubMed Central - PubMed

Affiliation: College of Life Science, Anhui Agricultural University, Anhui Hefei, P.R. China, 230036; Department of Pharmacology, Wannan Medical College, Anhui Wuhu, P.R.China, 241002.

ABSTRACT
Small heat shock proteins (sHSPs) can regulate protein folding and protect cells from stress. To investigate the role of sHSPs in the silk-producing insect Antheraea pernyi response to microorganisms, a sHsp gene termed as Ap-sHSP21.4, was identified. This gene encoded a 21.4 kDa protein which shares the conserved structure of insect sHsps and belongs to sHSP21.4 family. Ap-sHSP21.4 was highly expressed in fat body and up-regulated in midgut and fat body of A. pernyi challenged with Escherichia coli, Beauveria bassiana and nuclear polyhedrosis virus (NPV), which was determined by quantitative real-time PCR. Meanwhile, knock down of Ap-sHSP21.4 with dsRNA result in the decrease at the expression levels of several immune response-related genes (defensin, Dopa decarboxylase, Toll1, lysozyme and Kazal-type serine protease inhibitor). Additionally, the impact of eicosanoid biosynthesis on the expression of Ap-sHSP21.4 response to NPV was determined using qPCR, inhibitors of eicosanoid biosynthesis significantly suppress Ap-HSP21.4 expression upon NPV challenge. All together, Ap-sHSP21.4 was involved in the immunity of A. pernyi against microorganism and possibly mediated by eicosanoids pathway. These results will shed light in the understanding of the pathogen-host interaction in A. pernyi.

No MeSH data available.


Related in: MedlinePlus

Induction of Ap-sHSP21.4 by arachidonic acid in the fat body (A) and midgut (B).The larvae were injected with either 10 μL of DMSO (control), 10 μL of NPV (109 /larva) or arachidonic acid in 10 μL of DMSO (AA). The fat body (A) and midgut (B) 3 h or 24 h respectively after the second injection, The mRNA levels are shown as a percentage of the levels in the DMSO-treated control larvae. Bars represent the means ± SD (n = 5).
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4386827&req=5

pone.0121296.g005: Induction of Ap-sHSP21.4 by arachidonic acid in the fat body (A) and midgut (B).The larvae were injected with either 10 μL of DMSO (control), 10 μL of NPV (109 /larva) or arachidonic acid in 10 μL of DMSO (AA). The fat body (A) and midgut (B) 3 h or 24 h respectively after the second injection, The mRNA levels are shown as a percentage of the levels in the DMSO-treated control larvae. Bars represent the means ± SD (n = 5).

Mentions: The previous results strongly suggested that arachidonic acid metabolites mediated the induction of immune gene in the fat body, hemocyte and midgut. To test the direct effect of arachidonic acid metabolites on the induction, the larvae were treated with arachidonic acid, and the Ap-sHSP21.4 mRNA levels in the fat body and midgut were examined. As shown in Fig 5, the arachidonic acid could induce the gene expression, although the levels were somewhat lower than that induced by NPV. The increase in Ap-sHSP21.4 gene expression by arachidonic acid was significantly up-regulation (P>0.05) compared to that in the control (treated with DMSO).


Inhibitors of eicosanoid biosynthesis influencing the transcripts level of sHSP21.4 gene induced by pathogen infections, in Antheraea pernyi.

Zhang C, Dai L, Wang L, Qian C, Wei G, Li J, Zhu B, Liu C - PLoS ONE (2015)

Induction of Ap-sHSP21.4 by arachidonic acid in the fat body (A) and midgut (B).The larvae were injected with either 10 μL of DMSO (control), 10 μL of NPV (109 /larva) or arachidonic acid in 10 μL of DMSO (AA). The fat body (A) and midgut (B) 3 h or 24 h respectively after the second injection, The mRNA levels are shown as a percentage of the levels in the DMSO-treated control larvae. Bars represent the means ± SD (n = 5).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4386827&req=5

pone.0121296.g005: Induction of Ap-sHSP21.4 by arachidonic acid in the fat body (A) and midgut (B).The larvae were injected with either 10 μL of DMSO (control), 10 μL of NPV (109 /larva) or arachidonic acid in 10 μL of DMSO (AA). The fat body (A) and midgut (B) 3 h or 24 h respectively after the second injection, The mRNA levels are shown as a percentage of the levels in the DMSO-treated control larvae. Bars represent the means ± SD (n = 5).
Mentions: The previous results strongly suggested that arachidonic acid metabolites mediated the induction of immune gene in the fat body, hemocyte and midgut. To test the direct effect of arachidonic acid metabolites on the induction, the larvae were treated with arachidonic acid, and the Ap-sHSP21.4 mRNA levels in the fat body and midgut were examined. As shown in Fig 5, the arachidonic acid could induce the gene expression, although the levels were somewhat lower than that induced by NPV. The increase in Ap-sHSP21.4 gene expression by arachidonic acid was significantly up-regulation (P>0.05) compared to that in the control (treated with DMSO).

Bottom Line: Meanwhile, knock down of Ap-sHSP21.4 with dsRNA result in the decrease at the expression levels of several immune response-related genes (defensin, Dopa decarboxylase, Toll1, lysozyme and Kazal-type serine protease inhibitor).Additionally, the impact of eicosanoid biosynthesis on the expression of Ap-sHSP21.4 response to NPV was determined using qPCR, inhibitors of eicosanoid biosynthesis significantly suppress Ap-HSP21.4 expression upon NPV challenge.These results will shed light in the understanding of the pathogen-host interaction in A. pernyi.

View Article: PubMed Central - PubMed

Affiliation: College of Life Science, Anhui Agricultural University, Anhui Hefei, P.R. China, 230036; Department of Pharmacology, Wannan Medical College, Anhui Wuhu, P.R.China, 241002.

ABSTRACT
Small heat shock proteins (sHSPs) can regulate protein folding and protect cells from stress. To investigate the role of sHSPs in the silk-producing insect Antheraea pernyi response to microorganisms, a sHsp gene termed as Ap-sHSP21.4, was identified. This gene encoded a 21.4 kDa protein which shares the conserved structure of insect sHsps and belongs to sHSP21.4 family. Ap-sHSP21.4 was highly expressed in fat body and up-regulated in midgut and fat body of A. pernyi challenged with Escherichia coli, Beauveria bassiana and nuclear polyhedrosis virus (NPV), which was determined by quantitative real-time PCR. Meanwhile, knock down of Ap-sHSP21.4 with dsRNA result in the decrease at the expression levels of several immune response-related genes (defensin, Dopa decarboxylase, Toll1, lysozyme and Kazal-type serine protease inhibitor). Additionally, the impact of eicosanoid biosynthesis on the expression of Ap-sHSP21.4 response to NPV was determined using qPCR, inhibitors of eicosanoid biosynthesis significantly suppress Ap-HSP21.4 expression upon NPV challenge. All together, Ap-sHSP21.4 was involved in the immunity of A. pernyi against microorganism and possibly mediated by eicosanoids pathway. These results will shed light in the understanding of the pathogen-host interaction in A. pernyi.

No MeSH data available.


Related in: MedlinePlus