Limits...
Bronchodilatory and B-adrenergic effects of methanolic and aqueous extracts of Althaea root on isolated tracheobronchial smooth rat muscle.

Alani B, Zare M, Noureddini M - Adv Biomed Res (2015)

Bottom Line: The effect of methanolic and aqueous extracts of the root Althaea was assayed at different concentrations (0.2, 0.6, 2.6, 6.6, 14.6 μg/ml) and epinephrine (5 μm) in the presence and absence of propranolol (1 μM) under one g tension based on the isometric method.Propranolol inhibited the antispasmodic effect of epinephrine on tracheobronchial smooth muscle contractions, but could not reduce the antispasmodic effect of the root extract concentrations.Understanding the mechanism of this process can be useful in the treatment of pulmonary obstructive diseases like asthma.

View Article: PubMed Central - PubMed

Affiliation: Department of Genetic and Molecular Biology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran ; Department of Applied Cell Science, Kashan University of Medical Sciences, Kashan, Iran.

ABSTRACT

Background: The smooth muscle contractions of the tracheobronchial airways are mediated through the balance of adrenergic, cholinergic and peptidergic nervous mechanisms. This research was designed to determine the bronchodilatory and B-adrenergic effects of methanolic and aqueous extracts of root Althaea on the isolated tracheobronchial smooth muscle of the rat.

Materials and methods: In this experimental study, 116 tracheobronchial sections (5 mm) from 58 healthy male Sprague-Dawley rats were dissected and divided into 23 groups. The effect of methanolic and aqueous extracts of the root Althaea was assayed at different concentrations (0.2, 0.6, 2.6, 6.6, 14.6 μg/ml) and epinephrine (5 μm) in the presence and absence of propranolol (1 μM) under one g tension based on the isometric method. This assay was recorded in an organ bath containing Krebs-Henseleit solution for tracheobronchial smooth muscle contractions using potassium chloride (KCl) (60 mM) induction.

Results: Epinephrine (5 μm) alone and root methanolic and aqueous extract concentrations (0.6-14.6 μg/ml) reduced tracheobronchial smooth muscle contractions induced using KCl (60 mM) in a dose dependent manner. Propranolol inhibited the antispasmodic effect of epinephrine on tracheobronchial smooth muscle contractions, but could not reduce the antispasmodic effect of the root extract concentrations.

Conclusion: The methanolic and aqueous extracts of Althaea root inhibited the tracheobronchial smooth muscle contractions of rats in a dose dependent manner, but B-adrenergic receptors do not appear to engage in this process. Understanding the mechanism of this process can be useful in the treatment of pulmonary obstructive diseases like asthma.

No MeSH data available.


Related in: MedlinePlus

Comparison of the cumulative effect of Althaearoot aqueous and methanolic extracts at concentrations of 14.6, 6.6, 2.6, and 0.6 μg/ml with and without 1 μM propranolol on the contraction power of trachea muscle induced by potassium chloride (60 mM). The statistical differences in the data in the experimental groups were found using one-way analysis of variance and Tukey tests with SPSS-16 software; P<0.001 was considered as significant for all concentration
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4386210&req=5

Figure 1: Comparison of the cumulative effect of Althaearoot aqueous and methanolic extracts at concentrations of 14.6, 6.6, 2.6, and 0.6 μg/ml with and without 1 μM propranolol on the contraction power of trachea muscle induced by potassium chloride (60 mM). The statistical differences in the data in the experimental groups were found using one-way analysis of variance and Tukey tests with SPSS-16 software; P<0.001 was considered as significant for all concentration

Mentions: There was a significant dose dependent reduction in trachea smooth muscle contraction from both extracts; however, no significant difference was observed between the results of the effects of the individual extracts. To examine the agonistic effects of Althea root extracts, 0.6-14.6 μg/ml concentrations of aqueous and methanolic extracts were added with 1 μM propranolol to the medium. The results indicated that both extracts in synergy with propranolol still showed relaxation effects and the propranolol caused no significant change in antispasmodic effects of either extract [Figure 1].


Bronchodilatory and B-adrenergic effects of methanolic and aqueous extracts of Althaea root on isolated tracheobronchial smooth rat muscle.

Alani B, Zare M, Noureddini M - Adv Biomed Res (2015)

Comparison of the cumulative effect of Althaearoot aqueous and methanolic extracts at concentrations of 14.6, 6.6, 2.6, and 0.6 μg/ml with and without 1 μM propranolol on the contraction power of trachea muscle induced by potassium chloride (60 mM). The statistical differences in the data in the experimental groups were found using one-way analysis of variance and Tukey tests with SPSS-16 software; P<0.001 was considered as significant for all concentration
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4386210&req=5

Figure 1: Comparison of the cumulative effect of Althaearoot aqueous and methanolic extracts at concentrations of 14.6, 6.6, 2.6, and 0.6 μg/ml with and without 1 μM propranolol on the contraction power of trachea muscle induced by potassium chloride (60 mM). The statistical differences in the data in the experimental groups were found using one-way analysis of variance and Tukey tests with SPSS-16 software; P<0.001 was considered as significant for all concentration
Mentions: There was a significant dose dependent reduction in trachea smooth muscle contraction from both extracts; however, no significant difference was observed between the results of the effects of the individual extracts. To examine the agonistic effects of Althea root extracts, 0.6-14.6 μg/ml concentrations of aqueous and methanolic extracts were added with 1 μM propranolol to the medium. The results indicated that both extracts in synergy with propranolol still showed relaxation effects and the propranolol caused no significant change in antispasmodic effects of either extract [Figure 1].

Bottom Line: The effect of methanolic and aqueous extracts of the root Althaea was assayed at different concentrations (0.2, 0.6, 2.6, 6.6, 14.6 μg/ml) and epinephrine (5 μm) in the presence and absence of propranolol (1 μM) under one g tension based on the isometric method.Propranolol inhibited the antispasmodic effect of epinephrine on tracheobronchial smooth muscle contractions, but could not reduce the antispasmodic effect of the root extract concentrations.Understanding the mechanism of this process can be useful in the treatment of pulmonary obstructive diseases like asthma.

View Article: PubMed Central - PubMed

Affiliation: Department of Genetic and Molecular Biology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran ; Department of Applied Cell Science, Kashan University of Medical Sciences, Kashan, Iran.

ABSTRACT

Background: The smooth muscle contractions of the tracheobronchial airways are mediated through the balance of adrenergic, cholinergic and peptidergic nervous mechanisms. This research was designed to determine the bronchodilatory and B-adrenergic effects of methanolic and aqueous extracts of root Althaea on the isolated tracheobronchial smooth muscle of the rat.

Materials and methods: In this experimental study, 116 tracheobronchial sections (5 mm) from 58 healthy male Sprague-Dawley rats were dissected and divided into 23 groups. The effect of methanolic and aqueous extracts of the root Althaea was assayed at different concentrations (0.2, 0.6, 2.6, 6.6, 14.6 μg/ml) and epinephrine (5 μm) in the presence and absence of propranolol (1 μM) under one g tension based on the isometric method. This assay was recorded in an organ bath containing Krebs-Henseleit solution for tracheobronchial smooth muscle contractions using potassium chloride (KCl) (60 mM) induction.

Results: Epinephrine (5 μm) alone and root methanolic and aqueous extract concentrations (0.6-14.6 μg/ml) reduced tracheobronchial smooth muscle contractions induced using KCl (60 mM) in a dose dependent manner. Propranolol inhibited the antispasmodic effect of epinephrine on tracheobronchial smooth muscle contractions, but could not reduce the antispasmodic effect of the root extract concentrations.

Conclusion: The methanolic and aqueous extracts of Althaea root inhibited the tracheobronchial smooth muscle contractions of rats in a dose dependent manner, but B-adrenergic receptors do not appear to engage in this process. Understanding the mechanism of this process can be useful in the treatment of pulmonary obstructive diseases like asthma.

No MeSH data available.


Related in: MedlinePlus