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First Molecular Identification of Sarcocystis ovicanis (Protozoa, Apicomplexa) in the Brain of Sheep in Iran.

Salehi M, Bahari P, Vatanchian M - Iran J Parasitol (2014 Apr-Jun)

Bottom Line: Sarcocystis spp. was found in one of the brain samples (1.25%) using tissue digestion method.The presence of bradyzoite was also confirmed in the prepared histopathological sections.Sarcocystis ovicanis can involve brain tissue of sheep and consequently causes clinical symptoms.

View Article: PubMed Central - PubMed

Affiliation: Vector-Borne Diseases Research Center, North Khorasan University of Medical Sciences, Bojnurd, Iran.

ABSTRACT

Background: The objective of the present study was to survey the presence of Sarcocystis in sheep's brain in North Khorasan Province.

Methods: In general, 80 samples of sheep's brain were collected from slaughtered sheep in slaughterhouses of North Khorasan Province. Tissue digestion method was used for observing bradyzoites in tissues. Histopathological processing tracing Sarcocystis and ensuing structural change in the brain tissue were conducted. PCR analysis was conducted on all the brain samples. Sequencing was done for one PCR product. Genotype was identified by Blast search and homology analysis.

Result: Sarcocystis spp. was found in one of the brain samples (1.25%) using tissue digestion method. The presence of bradyzoite was also confirmed in the prepared histopathological sections. PCR analysis was positive in one of samples. Genotyping of one sample proved that Sarcocystis species was Sarcocystis ovicanis and the nucleotide sequence of this parasite was deposited in the GenBank database under accession number No.KF489431.

Conclusion: Sarcocystis ovicanis can involve brain tissue of sheep and consequently causes clinical symptoms.

No MeSH data available.


White matter of brain tissue sample(cross section of myelinated axons indicated by arrowheads) infected by bradyzoites of Sarcocystis ovicanis and some glial cell pyknoticnucleous(arrow). H&E,×100
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Figure 2: White matter of brain tissue sample(cross section of myelinated axons indicated by arrowheads) infected by bradyzoites of Sarcocystis ovicanis and some glial cell pyknoticnucleous(arrow). H&E,×100

Mentions: Histopathological analysis revealed that there were a few dispersed bradyzoites of S. ovicanis in different regions of the brain both in white and gray matter (Fig. 2, 3). These bradyzoites had elliptical shape and basophilic properties in H&E staining (Fig. 4, 5). There were a few pyknoticnucleous of neuroglia but, we saw no evidence of hemorrhage, perivascular lymphocytic cuffing, myelin sheath invasion by macrophage and encephalitis in histopathological analysis. Examinations of some regions of the brain (e.g. cerebellum) from histophathological view showed no structural changes and were completely free from bradyzoites.


First Molecular Identification of Sarcocystis ovicanis (Protozoa, Apicomplexa) in the Brain of Sheep in Iran.

Salehi M, Bahari P, Vatanchian M - Iran J Parasitol (2014 Apr-Jun)

White matter of brain tissue sample(cross section of myelinated axons indicated by arrowheads) infected by bradyzoites of Sarcocystis ovicanis and some glial cell pyknoticnucleous(arrow). H&E,×100
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4386052&req=5

Figure 2: White matter of brain tissue sample(cross section of myelinated axons indicated by arrowheads) infected by bradyzoites of Sarcocystis ovicanis and some glial cell pyknoticnucleous(arrow). H&E,×100
Mentions: Histopathological analysis revealed that there were a few dispersed bradyzoites of S. ovicanis in different regions of the brain both in white and gray matter (Fig. 2, 3). These bradyzoites had elliptical shape and basophilic properties in H&E staining (Fig. 4, 5). There were a few pyknoticnucleous of neuroglia but, we saw no evidence of hemorrhage, perivascular lymphocytic cuffing, myelin sheath invasion by macrophage and encephalitis in histopathological analysis. Examinations of some regions of the brain (e.g. cerebellum) from histophathological view showed no structural changes and were completely free from bradyzoites.

Bottom Line: Sarcocystis spp. was found in one of the brain samples (1.25%) using tissue digestion method.The presence of bradyzoite was also confirmed in the prepared histopathological sections.Sarcocystis ovicanis can involve brain tissue of sheep and consequently causes clinical symptoms.

View Article: PubMed Central - PubMed

Affiliation: Vector-Borne Diseases Research Center, North Khorasan University of Medical Sciences, Bojnurd, Iran.

ABSTRACT

Background: The objective of the present study was to survey the presence of Sarcocystis in sheep's brain in North Khorasan Province.

Methods: In general, 80 samples of sheep's brain were collected from slaughtered sheep in slaughterhouses of North Khorasan Province. Tissue digestion method was used for observing bradyzoites in tissues. Histopathological processing tracing Sarcocystis and ensuing structural change in the brain tissue were conducted. PCR analysis was conducted on all the brain samples. Sequencing was done for one PCR product. Genotype was identified by Blast search and homology analysis.

Result: Sarcocystis spp. was found in one of the brain samples (1.25%) using tissue digestion method. The presence of bradyzoite was also confirmed in the prepared histopathological sections. PCR analysis was positive in one of samples. Genotyping of one sample proved that Sarcocystis species was Sarcocystis ovicanis and the nucleotide sequence of this parasite was deposited in the GenBank database under accession number No.KF489431.

Conclusion: Sarcocystis ovicanis can involve brain tissue of sheep and consequently causes clinical symptoms.

No MeSH data available.