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First Molecular Identification of Sarcocystis ovicanis (Protozoa, Apicomplexa) in the Brain of Sheep in Iran.

Salehi M, Bahari P, Vatanchian M - Iran J Parasitol (2014 Apr-Jun)

Bottom Line: Sarcocystis spp. was found in one of the brain samples (1.25%) using tissue digestion method.The presence of bradyzoite was also confirmed in the prepared histopathological sections.Sarcocystis ovicanis can involve brain tissue of sheep and consequently causes clinical symptoms.

View Article: PubMed Central - PubMed

Affiliation: Vector-Borne Diseases Research Center, North Khorasan University of Medical Sciences, Bojnurd, Iran.

ABSTRACT

Background: The objective of the present study was to survey the presence of Sarcocystis in sheep's brain in North Khorasan Province.

Methods: In general, 80 samples of sheep's brain were collected from slaughtered sheep in slaughterhouses of North Khorasan Province. Tissue digestion method was used for observing bradyzoites in tissues. Histopathological processing tracing Sarcocystis and ensuing structural change in the brain tissue were conducted. PCR analysis was conducted on all the brain samples. Sequencing was done for one PCR product. Genotype was identified by Blast search and homology analysis.

Result: Sarcocystis spp. was found in one of the brain samples (1.25%) using tissue digestion method. The presence of bradyzoite was also confirmed in the prepared histopathological sections. PCR analysis was positive in one of samples. Genotyping of one sample proved that Sarcocystis species was Sarcocystis ovicanis and the nucleotide sequence of this parasite was deposited in the GenBank database under accession number No.KF489431.

Conclusion: Sarcocystis ovicanis can involve brain tissue of sheep and consequently causes clinical symptoms.

No MeSH data available.


Bradyzoite of Sarcocystis in sheep’s brain prepared by digestion method.×100
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Figure 1: Bradyzoite of Sarcocystis in sheep’s brain prepared by digestion method.×100

Mentions: Results obtained from tissue digestion method indicated that one of brain samples (1.25%) was infected with bradyzoites of Sarcocystis (Fig.1).


First Molecular Identification of Sarcocystis ovicanis (Protozoa, Apicomplexa) in the Brain of Sheep in Iran.

Salehi M, Bahari P, Vatanchian M - Iran J Parasitol (2014 Apr-Jun)

Bradyzoite of Sarcocystis in sheep’s brain prepared by digestion method.×100
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4386052&req=5

Figure 1: Bradyzoite of Sarcocystis in sheep’s brain prepared by digestion method.×100
Mentions: Results obtained from tissue digestion method indicated that one of brain samples (1.25%) was infected with bradyzoites of Sarcocystis (Fig.1).

Bottom Line: Sarcocystis spp. was found in one of the brain samples (1.25%) using tissue digestion method.The presence of bradyzoite was also confirmed in the prepared histopathological sections.Sarcocystis ovicanis can involve brain tissue of sheep and consequently causes clinical symptoms.

View Article: PubMed Central - PubMed

Affiliation: Vector-Borne Diseases Research Center, North Khorasan University of Medical Sciences, Bojnurd, Iran.

ABSTRACT

Background: The objective of the present study was to survey the presence of Sarcocystis in sheep's brain in North Khorasan Province.

Methods: In general, 80 samples of sheep's brain were collected from slaughtered sheep in slaughterhouses of North Khorasan Province. Tissue digestion method was used for observing bradyzoites in tissues. Histopathological processing tracing Sarcocystis and ensuing structural change in the brain tissue were conducted. PCR analysis was conducted on all the brain samples. Sequencing was done for one PCR product. Genotype was identified by Blast search and homology analysis.

Result: Sarcocystis spp. was found in one of the brain samples (1.25%) using tissue digestion method. The presence of bradyzoite was also confirmed in the prepared histopathological sections. PCR analysis was positive in one of samples. Genotyping of one sample proved that Sarcocystis species was Sarcocystis ovicanis and the nucleotide sequence of this parasite was deposited in the GenBank database under accession number No.KF489431.

Conclusion: Sarcocystis ovicanis can involve brain tissue of sheep and consequently causes clinical symptoms.

No MeSH data available.