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Hydroxy-α sanshool induces colonic motor activity in rat proximal colon: a possible involvement of KCNK9.

Kubota K, Ohtake N, Ohbuchi K, Mase A, Imamura S, Sudo Y, Miyano K, Yamamoto M, Kono T, Uezono Y - Am. J. Physiol. Gastrointest. Liver Physiol. (2015)

Bottom Line: Hydroxy-α sanshool (HAS), a readily absorbable active ingredient of TU-100 and a KCNK3/KCNK9/KCNK18 blocker as well as TRPV1/TRPA1 agonist, has been investigated for its effects on colonic motility.The effect of HAS was unaltered by TRPV1/TRPA1 antagonists and desensitization.HAS may evoke strong LDC possibly via blockage of the neural KCNK9 channel in the colonic myenteric plexus.

View Article: PubMed Central - PubMed

Affiliation: Tsumura Research Laboratories, Tsumura & Co., Ibaraki, Japan;

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Related in: MedlinePlus

Acceleration of defecation by HAS and TU-100 in normal rats or postoperative ileus (POI) model rats. A: increase of defecation frequency at 5 h after HAS (50 mg/kg po) administration in normal rats. *P < 0.05, **P < 0.01 vs. Vehicle (olive oil 0.5 ml/kg po) (n = 8). B: decrease of defecation frequency by laparotomy. *P < 0.05, ***P < 0.001 vs. Normal (anesthetized only). (n = 8 ∼21, at 7 h after operation). C: increase of defecation frequency after HAS (15 and 50 mg/kg po) dosing at 4 h after administration (i.e., 6 h after operation) in POI rats. *P < 0.05 vs. Vehicle (n = 23–25). D: acceleration of defecation frequency induced TU-100 (1 and 3 g/kg po) dosing 3 h after administration (i.e., 6 h after operation) in POI rats. *P < 0.05, **P < 0.01 vs. Vehicle (water 15 ml/kg po) (n = 8 or 9).
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Figure 9: Acceleration of defecation by HAS and TU-100 in normal rats or postoperative ileus (POI) model rats. A: increase of defecation frequency at 5 h after HAS (50 mg/kg po) administration in normal rats. *P < 0.05, **P < 0.01 vs. Vehicle (olive oil 0.5 ml/kg po) (n = 8). B: decrease of defecation frequency by laparotomy. *P < 0.05, ***P < 0.001 vs. Normal (anesthetized only). (n = 8 ∼21, at 7 h after operation). C: increase of defecation frequency after HAS (15 and 50 mg/kg po) dosing at 4 h after administration (i.e., 6 h after operation) in POI rats. *P < 0.05 vs. Vehicle (n = 23–25). D: acceleration of defecation frequency induced TU-100 (1 and 3 g/kg po) dosing 3 h after administration (i.e., 6 h after operation) in POI rats. *P < 0.05, **P < 0.01 vs. Vehicle (water 15 ml/kg po) (n = 8 or 9).

Mentions: To investigate whether HAS accelerates defecation in vivo we examined the amount of feces accumulated during a short period after treatment with the agents. The accumulated number of fecal pellets increased significantly at 5 h after oral administration of 50 mg/kg HAS to normal rats (Fig. 9A).


Hydroxy-α sanshool induces colonic motor activity in rat proximal colon: a possible involvement of KCNK9.

Kubota K, Ohtake N, Ohbuchi K, Mase A, Imamura S, Sudo Y, Miyano K, Yamamoto M, Kono T, Uezono Y - Am. J. Physiol. Gastrointest. Liver Physiol. (2015)

Acceleration of defecation by HAS and TU-100 in normal rats or postoperative ileus (POI) model rats. A: increase of defecation frequency at 5 h after HAS (50 mg/kg po) administration in normal rats. *P < 0.05, **P < 0.01 vs. Vehicle (olive oil 0.5 ml/kg po) (n = 8). B: decrease of defecation frequency by laparotomy. *P < 0.05, ***P < 0.001 vs. Normal (anesthetized only). (n = 8 ∼21, at 7 h after operation). C: increase of defecation frequency after HAS (15 and 50 mg/kg po) dosing at 4 h after administration (i.e., 6 h after operation) in POI rats. *P < 0.05 vs. Vehicle (n = 23–25). D: acceleration of defecation frequency induced TU-100 (1 and 3 g/kg po) dosing 3 h after administration (i.e., 6 h after operation) in POI rats. *P < 0.05, **P < 0.01 vs. Vehicle (water 15 ml/kg po) (n = 8 or 9).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4385894&req=5

Figure 9: Acceleration of defecation by HAS and TU-100 in normal rats or postoperative ileus (POI) model rats. A: increase of defecation frequency at 5 h after HAS (50 mg/kg po) administration in normal rats. *P < 0.05, **P < 0.01 vs. Vehicle (olive oil 0.5 ml/kg po) (n = 8). B: decrease of defecation frequency by laparotomy. *P < 0.05, ***P < 0.001 vs. Normal (anesthetized only). (n = 8 ∼21, at 7 h after operation). C: increase of defecation frequency after HAS (15 and 50 mg/kg po) dosing at 4 h after administration (i.e., 6 h after operation) in POI rats. *P < 0.05 vs. Vehicle (n = 23–25). D: acceleration of defecation frequency induced TU-100 (1 and 3 g/kg po) dosing 3 h after administration (i.e., 6 h after operation) in POI rats. *P < 0.05, **P < 0.01 vs. Vehicle (water 15 ml/kg po) (n = 8 or 9).
Mentions: To investigate whether HAS accelerates defecation in vivo we examined the amount of feces accumulated during a short period after treatment with the agents. The accumulated number of fecal pellets increased significantly at 5 h after oral administration of 50 mg/kg HAS to normal rats (Fig. 9A).

Bottom Line: Hydroxy-α sanshool (HAS), a readily absorbable active ingredient of TU-100 and a KCNK3/KCNK9/KCNK18 blocker as well as TRPV1/TRPA1 agonist, has been investigated for its effects on colonic motility.The effect of HAS was unaltered by TRPV1/TRPA1 antagonists and desensitization.HAS may evoke strong LDC possibly via blockage of the neural KCNK9 channel in the colonic myenteric plexus.

View Article: PubMed Central - PubMed

Affiliation: Tsumura Research Laboratories, Tsumura & Co., Ibaraki, Japan;

Show MeSH
Related in: MedlinePlus