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Hydroxy-α sanshool induces colonic motor activity in rat proximal colon: a possible involvement of KCNK9.

Kubota K, Ohtake N, Ohbuchi K, Mase A, Imamura S, Sudo Y, Miyano K, Yamamoto M, Kono T, Uezono Y - Am. J. Physiol. Gastrointest. Liver Physiol. (2015)

Bottom Line: Hydroxy-α sanshool (HAS), a readily absorbable active ingredient of TU-100 and a KCNK3/KCNK9/KCNK18 blocker as well as TRPV1/TRPA1 agonist, has been investigated for its effects on colonic motility.The effect of HAS was unaltered by TRPV1/TRPA1 antagonists and desensitization.HAS may evoke strong LDC possibly via blockage of the neural KCNK9 channel in the colonic myenteric plexus.

View Article: PubMed Central - PubMed

Affiliation: Tsumura Research Laboratories, Tsumura & Co., Ibaraki, Japan;

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Related in: MedlinePlus

Effect of TTX on HAS- and HBS-induced contraction. A and B: top charts show typical changes in the HAS (left) and HBS (right) motility patterns induced by TTX. Middle and bottom graphs show changes of %-PF, %-PPA, and %-AUC induced by TTX, respectively. Experiment used 10 μM HAS (n = 6) and 30 μM HBS (n = 5 and 6). Pre means the motility before addition of TTX or its vehicle. *P < 0.05, **P < 0.01 vs. Control (vehicle).
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Figure 8: Effect of TTX on HAS- and HBS-induced contraction. A and B: top charts show typical changes in the HAS (left) and HBS (right) motility patterns induced by TTX. Middle and bottom graphs show changes of %-PF, %-PPA, and %-AUC induced by TTX, respectively. Experiment used 10 μM HAS (n = 6) and 30 μM HBS (n = 5 and 6). Pre means the motility before addition of TTX or its vehicle. *P < 0.05, **P < 0.01 vs. Control (vehicle).

Mentions: The contractions induced by 10 μM HAS were suppressed almost completely by addition of TTX (0.3 μM), a neuroleptic agent (Fig. 8A). By contrast, the contractions induced by HBS were not abolished but its shape was changed by TTX (Fig. 8B). Addition of high doses (up to 3 mM) of TTX did not alter the effect of Lid (1 mM, data not shown).


Hydroxy-α sanshool induces colonic motor activity in rat proximal colon: a possible involvement of KCNK9.

Kubota K, Ohtake N, Ohbuchi K, Mase A, Imamura S, Sudo Y, Miyano K, Yamamoto M, Kono T, Uezono Y - Am. J. Physiol. Gastrointest. Liver Physiol. (2015)

Effect of TTX on HAS- and HBS-induced contraction. A and B: top charts show typical changes in the HAS (left) and HBS (right) motility patterns induced by TTX. Middle and bottom graphs show changes of %-PF, %-PPA, and %-AUC induced by TTX, respectively. Experiment used 10 μM HAS (n = 6) and 30 μM HBS (n = 5 and 6). Pre means the motility before addition of TTX or its vehicle. *P < 0.05, **P < 0.01 vs. Control (vehicle).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4385894&req=5

Figure 8: Effect of TTX on HAS- and HBS-induced contraction. A and B: top charts show typical changes in the HAS (left) and HBS (right) motility patterns induced by TTX. Middle and bottom graphs show changes of %-PF, %-PPA, and %-AUC induced by TTX, respectively. Experiment used 10 μM HAS (n = 6) and 30 μM HBS (n = 5 and 6). Pre means the motility before addition of TTX or its vehicle. *P < 0.05, **P < 0.01 vs. Control (vehicle).
Mentions: The contractions induced by 10 μM HAS were suppressed almost completely by addition of TTX (0.3 μM), a neuroleptic agent (Fig. 8A). By contrast, the contractions induced by HBS were not abolished but its shape was changed by TTX (Fig. 8B). Addition of high doses (up to 3 mM) of TTX did not alter the effect of Lid (1 mM, data not shown).

Bottom Line: Hydroxy-α sanshool (HAS), a readily absorbable active ingredient of TU-100 and a KCNK3/KCNK9/KCNK18 blocker as well as TRPV1/TRPA1 agonist, has been investigated for its effects on colonic motility.The effect of HAS was unaltered by TRPV1/TRPA1 antagonists and desensitization.HAS may evoke strong LDC possibly via blockage of the neural KCNK9 channel in the colonic myenteric plexus.

View Article: PubMed Central - PubMed

Affiliation: Tsumura Research Laboratories, Tsumura & Co., Ibaraki, Japan;

Show MeSH
Related in: MedlinePlus