Hydroxy-α sanshool induces colonic motor activity in rat proximal colon: a possible involvement of KCNK9.
Bottom Line: Hydroxy-α sanshool (HAS), a readily absorbable active ingredient of TU-100 and a KCNK3/KCNK9/KCNK18 blocker as well as TRPV1/TRPA1 agonist, has been investigated for its effects on colonic motility.The effect of HAS was unaltered by TRPV1/TRPA1 antagonists and desensitization.HAS may evoke strong LDC possibly via blockage of the neural KCNK9 channel in the colonic myenteric plexus.
Affiliation: Tsumura Research Laboratories, Tsumura & Co., Ibaraki, Japan;Show MeSH
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Mentions: Although HAS, HBS, and Lid raised the intraluminal pressure of rat isolated colons and induced potent contraction/motility, the resulting motor patterns differed among these three compounds. Although the pressure peaks induced by HAS were strong and repeated periodically with almost the same frequency, those induced by HBS were lower in frequency and less periodic (Fig. 7A, Supplementary Video S6). The effects induced by Lid were also weaker in amplitude, not periodic, but higher in frequency than those induced by HAS (Fig. 7B, Supplementary Video S7). The spatiotemporal maps also suggested that HAS induces potent motor activity propagating from the oral side to the aboral side over the entire proximal colon (Fig. 1A), whereas Lid was found to induce high-frequency, low-amplitude, nonpropulsive motor activity (Fig. 7B). Table 1 shows the PF of HAS-, HBS-, and Lid-induced colonic motility.
Affiliation: Tsumura Research Laboratories, Tsumura & Co., Ibaraki, Japan;