Hydroxy-α sanshool induces colonic motor activity in rat proximal colon: a possible involvement of KCNK9.
Bottom Line: Hydroxy-α sanshool (HAS), a readily absorbable active ingredient of TU-100 and a KCNK3/KCNK9/KCNK18 blocker as well as TRPV1/TRPA1 agonist, has been investigated for its effects on colonic motility.The effect of HAS was unaltered by TRPV1/TRPA1 antagonists and desensitization.HAS may evoke strong LDC possibly via blockage of the neural KCNK9 channel in the colonic myenteric plexus.
Affiliation: Tsumura Research Laboratories, Tsumura & Co., Ibaraki, Japan;Show MeSH
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Mentions: As shown in Fig. 1A, addition of HAS to the bath solution from the serosal side at 30 min and 3 h induced high-amplitude periodic pressure peaks with similar frequency and potency. The motility is characterized by a strong squeezing over a broad range of the proximal colon (Fig. 1, B and C, movie files are shown in Supplementary Video S3), after a brief temporal relaxation (Fig. 1C). The dose dependency of pressure peak pattern, PF, PPA, and AUC are shown in Fig. 2.
Affiliation: Tsumura Research Laboratories, Tsumura & Co., Ibaraki, Japan;