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Hydroxy-α sanshool induces colonic motor activity in rat proximal colon: a possible involvement of KCNK9.

Kubota K, Ohtake N, Ohbuchi K, Mase A, Imamura S, Sudo Y, Miyano K, Yamamoto M, Kono T, Uezono Y - Am. J. Physiol. Gastrointest. Liver Physiol. (2015)

Bottom Line: Hydroxy-α sanshool (HAS), a readily absorbable active ingredient of TU-100 and a KCNK3/KCNK9/KCNK18 blocker as well as TRPV1/TRPA1 agonist, has been investigated for its effects on colonic motility.The effect of HAS was unaltered by TRPV1/TRPA1 antagonists and desensitization.HAS may evoke strong LDC possibly via blockage of the neural KCNK9 channel in the colonic myenteric plexus.

View Article: PubMed Central - PubMed

Affiliation: Tsumura Research Laboratories, Tsumura & Co., Ibaraki, Japan;

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Related in: MedlinePlus

Dose-dependent induction of high-amplitude pressure peaks by HAS. A: typical patterns of intraluminal pressure induced by HAS. B: quantitation of HAS-induced contractility. Ratio of peak frequency (PF), peak pressure amplitude (PPA), and area under the curve (AUC) calculated as the ratio to the PF, PPA, and AUC before drug treatment (%-PF, %-PPA, and %-AUC, respectively) are shown (n = 4–6). *P < 0.05, **P < 0.01, ***P < 0.001 vs. Vehicle control.
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Figure 2: Dose-dependent induction of high-amplitude pressure peaks by HAS. A: typical patterns of intraluminal pressure induced by HAS. B: quantitation of HAS-induced contractility. Ratio of peak frequency (PF), peak pressure amplitude (PPA), and area under the curve (AUC) calculated as the ratio to the PF, PPA, and AUC before drug treatment (%-PF, %-PPA, and %-AUC, respectively) are shown (n = 4–6). *P < 0.05, **P < 0.01, ***P < 0.001 vs. Vehicle control.

Mentions: As shown in Fig. 1A, addition of HAS to the bath solution from the serosal side at 30 min and 3 h induced high-amplitude periodic pressure peaks with similar frequency and potency. The motility is characterized by a strong squeezing over a broad range of the proximal colon (Fig. 1, B and C, movie files are shown in Supplementary Video S3), after a brief temporal relaxation (Fig. 1C). The dose dependency of pressure peak pattern, PF, PPA, and AUC are shown in Fig. 2.


Hydroxy-α sanshool induces colonic motor activity in rat proximal colon: a possible involvement of KCNK9.

Kubota K, Ohtake N, Ohbuchi K, Mase A, Imamura S, Sudo Y, Miyano K, Yamamoto M, Kono T, Uezono Y - Am. J. Physiol. Gastrointest. Liver Physiol. (2015)

Dose-dependent induction of high-amplitude pressure peaks by HAS. A: typical patterns of intraluminal pressure induced by HAS. B: quantitation of HAS-induced contractility. Ratio of peak frequency (PF), peak pressure amplitude (PPA), and area under the curve (AUC) calculated as the ratio to the PF, PPA, and AUC before drug treatment (%-PF, %-PPA, and %-AUC, respectively) are shown (n = 4–6). *P < 0.05, **P < 0.01, ***P < 0.001 vs. Vehicle control.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4385894&req=5

Figure 2: Dose-dependent induction of high-amplitude pressure peaks by HAS. A: typical patterns of intraluminal pressure induced by HAS. B: quantitation of HAS-induced contractility. Ratio of peak frequency (PF), peak pressure amplitude (PPA), and area under the curve (AUC) calculated as the ratio to the PF, PPA, and AUC before drug treatment (%-PF, %-PPA, and %-AUC, respectively) are shown (n = 4–6). *P < 0.05, **P < 0.01, ***P < 0.001 vs. Vehicle control.
Mentions: As shown in Fig. 1A, addition of HAS to the bath solution from the serosal side at 30 min and 3 h induced high-amplitude periodic pressure peaks with similar frequency and potency. The motility is characterized by a strong squeezing over a broad range of the proximal colon (Fig. 1, B and C, movie files are shown in Supplementary Video S3), after a brief temporal relaxation (Fig. 1C). The dose dependency of pressure peak pattern, PF, PPA, and AUC are shown in Fig. 2.

Bottom Line: Hydroxy-α sanshool (HAS), a readily absorbable active ingredient of TU-100 and a KCNK3/KCNK9/KCNK18 blocker as well as TRPV1/TRPA1 agonist, has been investigated for its effects on colonic motility.The effect of HAS was unaltered by TRPV1/TRPA1 antagonists and desensitization.HAS may evoke strong LDC possibly via blockage of the neural KCNK9 channel in the colonic myenteric plexus.

View Article: PubMed Central - PubMed

Affiliation: Tsumura Research Laboratories, Tsumura & Co., Ibaraki, Japan;

Show MeSH
Related in: MedlinePlus