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Immunohistochemical quantification of the cobalamin transport protein, cell surface receptor and Ki-67 in naturally occurring canine and feline malignant tumors and in adjacent normal tissues.

Sysel AM, Valli VE, Bauer JA - Oncotarget (2015)

Bottom Line: This study quantified the immunohistochemical expression of the cobalamin transport protein (transcobalamin II; TCII), cell surface receptor (transcobalamin II-R; TCII-R) and proliferation protein (Ki-67) in naturally occurring canine and feline malignant tumors, and compared these results to expression in corresponding adjacent normal tissues.Expression of TCII, TCII-R and Ki-67 was significantly higher in malignant tumor tissues than in corresponding adjacent normal tissues in both species.These results demonstrate a quantifiable, synchronous up-regulation of TCII and TCII-R expression by proliferating canine and feline malignant tumors.

View Article: PubMed Central - PubMed

Affiliation: Bauer Research Foundation, Akron, Ohio, USA.

ABSTRACT
Cancer cells have an obligate need for cobalamin (vitamin B12) to enable DNA synthesis necessary for cellular replication. This study quantified the immunohistochemical expression of the cobalamin transport protein (transcobalamin II; TCII), cell surface receptor (transcobalamin II-R; TCII-R) and proliferation protein (Ki-67) in naturally occurring canine and feline malignant tumors, and compared these results to expression in corresponding adjacent normal tissues. All malignant tumor tissues stained positively for TCII, TCII-R and Ki-67 proteins; expression varied both within and between tumor types. Expression of TCII, TCII-R and Ki-67 was significantly higher in malignant tumor tissues than in corresponding adjacent normal tissues in both species. There was a strong correlation between TCII and TCII-R expression, and a modest correlation between TCII-R and Ki-67 expression in both species; a modest association between TCII and Ki-67 expression was present in canine tissues only. These results demonstrate a quantifiable, synchronous up-regulation of TCII and TCII-R expression by proliferating canine and feline malignant tumors. The potential to utilize these proteins as biomarkers to identify neoplastic tissues, streamline therapeutic options, evaluate response to anti-tumor therapy and monitor for recurrent disease has important implications in the advancement of cancer management for both human and companion animal patients.

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Digital images of stained feline tissue sectionsTissue samples were immunohistochemically stained for TCII (TCN2 antibody, 10x objective), TCII-R (CD320 antibody, 10x objective) and Ki-67 (MIB-1 antibody, 40x objective). Tissue sections for each case are shown from left to right as follows: TCII tumor, TCII adjacent normal, TCII-R tumor, TCII-R adjacent normal, Ki-67 tumor, Ki-67 adjacent normal. Refer to Figure 3 legend for x-axis case identification. High resolution images of case 36 are presented in Supplemental data.
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Figure 4: Digital images of stained feline tissue sectionsTissue samples were immunohistochemically stained for TCII (TCN2 antibody, 10x objective), TCII-R (CD320 antibody, 10x objective) and Ki-67 (MIB-1 antibody, 40x objective). Tissue sections for each case are shown from left to right as follows: TCII tumor, TCII adjacent normal, TCII-R tumor, TCII-R adjacent normal, Ki-67 tumor, Ki-67 adjacent normal. Refer to Figure 3 legend for x-axis case identification. High resolution images of case 36 are presented in Supplemental data.

Mentions: Immunohistochemical staining values for feline tissues are summarized in Table 1 and illustrated graphically in Figure 3; digital images of all stained slides are shown in Figure 4.


Immunohistochemical quantification of the cobalamin transport protein, cell surface receptor and Ki-67 in naturally occurring canine and feline malignant tumors and in adjacent normal tissues.

Sysel AM, Valli VE, Bauer JA - Oncotarget (2015)

Digital images of stained feline tissue sectionsTissue samples were immunohistochemically stained for TCII (TCN2 antibody, 10x objective), TCII-R (CD320 antibody, 10x objective) and Ki-67 (MIB-1 antibody, 40x objective). Tissue sections for each case are shown from left to right as follows: TCII tumor, TCII adjacent normal, TCII-R tumor, TCII-R adjacent normal, Ki-67 tumor, Ki-67 adjacent normal. Refer to Figure 3 legend for x-axis case identification. High resolution images of case 36 are presented in Supplemental data.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4385855&req=5

Figure 4: Digital images of stained feline tissue sectionsTissue samples were immunohistochemically stained for TCII (TCN2 antibody, 10x objective), TCII-R (CD320 antibody, 10x objective) and Ki-67 (MIB-1 antibody, 40x objective). Tissue sections for each case are shown from left to right as follows: TCII tumor, TCII adjacent normal, TCII-R tumor, TCII-R adjacent normal, Ki-67 tumor, Ki-67 adjacent normal. Refer to Figure 3 legend for x-axis case identification. High resolution images of case 36 are presented in Supplemental data.
Mentions: Immunohistochemical staining values for feline tissues are summarized in Table 1 and illustrated graphically in Figure 3; digital images of all stained slides are shown in Figure 4.

Bottom Line: This study quantified the immunohistochemical expression of the cobalamin transport protein (transcobalamin II; TCII), cell surface receptor (transcobalamin II-R; TCII-R) and proliferation protein (Ki-67) in naturally occurring canine and feline malignant tumors, and compared these results to expression in corresponding adjacent normal tissues.Expression of TCII, TCII-R and Ki-67 was significantly higher in malignant tumor tissues than in corresponding adjacent normal tissues in both species.These results demonstrate a quantifiable, synchronous up-regulation of TCII and TCII-R expression by proliferating canine and feline malignant tumors.

View Article: PubMed Central - PubMed

Affiliation: Bauer Research Foundation, Akron, Ohio, USA.

ABSTRACT
Cancer cells have an obligate need for cobalamin (vitamin B12) to enable DNA synthesis necessary for cellular replication. This study quantified the immunohistochemical expression of the cobalamin transport protein (transcobalamin II; TCII), cell surface receptor (transcobalamin II-R; TCII-R) and proliferation protein (Ki-67) in naturally occurring canine and feline malignant tumors, and compared these results to expression in corresponding adjacent normal tissues. All malignant tumor tissues stained positively for TCII, TCII-R and Ki-67 proteins; expression varied both within and between tumor types. Expression of TCII, TCII-R and Ki-67 was significantly higher in malignant tumor tissues than in corresponding adjacent normal tissues in both species. There was a strong correlation between TCII and TCII-R expression, and a modest correlation between TCII-R and Ki-67 expression in both species; a modest association between TCII and Ki-67 expression was present in canine tissues only. These results demonstrate a quantifiable, synchronous up-regulation of TCII and TCII-R expression by proliferating canine and feline malignant tumors. The potential to utilize these proteins as biomarkers to identify neoplastic tissues, streamline therapeutic options, evaluate response to anti-tumor therapy and monitor for recurrent disease has important implications in the advancement of cancer management for both human and companion animal patients.

Show MeSH
Related in: MedlinePlus