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The antimicrobial peptide pardaxin exerts potent anti-tumor activity against canine perianal gland adenoma.

Pan CY, Lin CN, Chiou MT, Yu CY, Chen JY, Chien CH - Oncotarget (2015)

Bottom Line: Local injection of pardaxin resulted in a significant reduction of perianal gland adenoma growth between 28 and 38 days post-treatment.Surgical resection of canine histiocytomas revealed large areas of ulceration, suggesting that pardaxin acts like a lytic peptide.Pardaxin treatment was not associated with significant variations in blood biochemical parameters or secretion of immune-related proteins.

View Article: PubMed Central - PubMed

Affiliation: Department and Graduate Institute of Aquaculture, National Kaohsiung Marine University, Nanzih Dist., Kaohsiung, Taiwan.

ABSTRACT
Pardaxin is an antimicrobial peptide of 33 amino acids, originally isolated from marine fish. We previously demonstrated that pardaxin has anti-tumor activity against murine fibrosarcoma, both in vitro and in vivo. In this study, we examined the anti-tumor activity, toxicity profile, and maximally-tolerated dose of pardaxin treatment in dogs with different types of refractory tumor. Local injection of pardaxin resulted in a significant reduction of perianal gland adenoma growth between 28 and 38 days post-treatment. Surgical resection of canine histiocytomas revealed large areas of ulceration, suggesting that pardaxin acts like a lytic peptide. Pardaxin treatment was not associated with significant variations in blood biochemical parameters or secretion of immune-related proteins. Our findings indicate that pardaxin has strong therapeutic potential for treating perianal gland adenomas in dogs. These data justify the veterinary application of pardaxin, and also provide invaluable information for veterinary medicine and future human clinical trials.

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Related in: MedlinePlus

Change in tumor volume during treatment with pardaxin(a) Two histiocytoma tumors (of dogs #7 and #10) were monitored for 28 days. (b) (A) Prior to treatment on day 0 in dog #10. (B) Histiocytoma tumor after 19 days of pardaxin treatment. (C) Surgical excision of the tumor mass after 28 days of treatment with pardaxin; pus can be seen in the capsule, and there was no substantial tumor mass. The mass was hollow with extensive areas of necrosis after 28 days of daily intra-tumoral administration of pardaxin.
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Figure 4: Change in tumor volume during treatment with pardaxin(a) Two histiocytoma tumors (of dogs #7 and #10) were monitored for 28 days. (b) (A) Prior to treatment on day 0 in dog #10. (B) Histiocytoma tumor after 19 days of pardaxin treatment. (C) Surgical excision of the tumor mass after 28 days of treatment with pardaxin; pus can be seen in the capsule, and there was no substantial tumor mass. The mass was hollow with extensive areas of necrosis after 28 days of daily intra-tumoral administration of pardaxin.

Mentions: Dog patients #7 and #10 bore histiocytomas, which were not substantially affected by pardaxin treatment (Fig. 4a, Table 2). Dog #10, a 2-year-old American Pit Bull Terrier, presented with a histiocytoma in the left neck (4.400 cm2) (Fig. 4b). The dog received a single daily intratumoral injection of 10 mg/ml pardaxin (2 ml/day) dissolved in PBS. At day 19, a significant increase in tumor volume was observed, and the tumor mass felt very soft to the touch (Fig. 4b). Pardaxin treatment was halted at day 28. Subsequently, the tumor surface exhibited a small area of cyanosis (Fig. 4b). Surgical excision of the mass after the 28 days of pardaxin treatment revealed pus in the capsule and no significant tumor mass (Fig. 4b). The mass was hollow, with extensive areas of necrosis.


The antimicrobial peptide pardaxin exerts potent anti-tumor activity against canine perianal gland adenoma.

Pan CY, Lin CN, Chiou MT, Yu CY, Chen JY, Chien CH - Oncotarget (2015)

Change in tumor volume during treatment with pardaxin(a) Two histiocytoma tumors (of dogs #7 and #10) were monitored for 28 days. (b) (A) Prior to treatment on day 0 in dog #10. (B) Histiocytoma tumor after 19 days of pardaxin treatment. (C) Surgical excision of the tumor mass after 28 days of treatment with pardaxin; pus can be seen in the capsule, and there was no substantial tumor mass. The mass was hollow with extensive areas of necrosis after 28 days of daily intra-tumoral administration of pardaxin.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4385852&req=5

Figure 4: Change in tumor volume during treatment with pardaxin(a) Two histiocytoma tumors (of dogs #7 and #10) were monitored for 28 days. (b) (A) Prior to treatment on day 0 in dog #10. (B) Histiocytoma tumor after 19 days of pardaxin treatment. (C) Surgical excision of the tumor mass after 28 days of treatment with pardaxin; pus can be seen in the capsule, and there was no substantial tumor mass. The mass was hollow with extensive areas of necrosis after 28 days of daily intra-tumoral administration of pardaxin.
Mentions: Dog patients #7 and #10 bore histiocytomas, which were not substantially affected by pardaxin treatment (Fig. 4a, Table 2). Dog #10, a 2-year-old American Pit Bull Terrier, presented with a histiocytoma in the left neck (4.400 cm2) (Fig. 4b). The dog received a single daily intratumoral injection of 10 mg/ml pardaxin (2 ml/day) dissolved in PBS. At day 19, a significant increase in tumor volume was observed, and the tumor mass felt very soft to the touch (Fig. 4b). Pardaxin treatment was halted at day 28. Subsequently, the tumor surface exhibited a small area of cyanosis (Fig. 4b). Surgical excision of the mass after the 28 days of pardaxin treatment revealed pus in the capsule and no significant tumor mass (Fig. 4b). The mass was hollow, with extensive areas of necrosis.

Bottom Line: Local injection of pardaxin resulted in a significant reduction of perianal gland adenoma growth between 28 and 38 days post-treatment.Surgical resection of canine histiocytomas revealed large areas of ulceration, suggesting that pardaxin acts like a lytic peptide.Pardaxin treatment was not associated with significant variations in blood biochemical parameters or secretion of immune-related proteins.

View Article: PubMed Central - PubMed

Affiliation: Department and Graduate Institute of Aquaculture, National Kaohsiung Marine University, Nanzih Dist., Kaohsiung, Taiwan.

ABSTRACT
Pardaxin is an antimicrobial peptide of 33 amino acids, originally isolated from marine fish. We previously demonstrated that pardaxin has anti-tumor activity against murine fibrosarcoma, both in vitro and in vivo. In this study, we examined the anti-tumor activity, toxicity profile, and maximally-tolerated dose of pardaxin treatment in dogs with different types of refractory tumor. Local injection of pardaxin resulted in a significant reduction of perianal gland adenoma growth between 28 and 38 days post-treatment. Surgical resection of canine histiocytomas revealed large areas of ulceration, suggesting that pardaxin acts like a lytic peptide. Pardaxin treatment was not associated with significant variations in blood biochemical parameters or secretion of immune-related proteins. Our findings indicate that pardaxin has strong therapeutic potential for treating perianal gland adenomas in dogs. These data justify the veterinary application of pardaxin, and also provide invaluable information for veterinary medicine and future human clinical trials.

Show MeSH
Related in: MedlinePlus