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Mesenchymal stem cells are resistant to carbon ion radiotherapy.

Nicolay NH, Liang Y, Lopez Perez R, Bostel T, Trinh T, Sisombath S, Weber KJ, Ho AD, Debus J, Saffrich R, Huber PE - Oncotarget (2015)

Bottom Line: Here we show that cellular morphology, adhesion and migration abilities were not measurably altered by photon or carbon ion irradiation.We have shown for the first time that MSCs are relatively resistant to therapeutic carbon ion radiotherapy.Additionally, this form of radiation did not markedly alter the defining stem cell properties or the expression of established surface markers in MSCs.

View Article: PubMed Central - PubMed

Affiliation: Department of Radiation Oncology, Heidelberg University Hospital, Im Neuenheimer Feld, Heidelberg, Germany.

ABSTRACT
Mesenchymal stem cells (MSCs) participate in regeneration of tissues damaged by ionizing radiation. However, radiation can damage MSCs themselves. Here we show that cellular morphology, adhesion and migration abilities were not measurably altered by photon or carbon ion irradiation. The potential for differentiation was unaffected by either form of radiation, and established MSC surface markers were found to be stably expressed irrespective of radiation treatment. MSCs were able to efficiently repair DNA double strand breaks induced by both high-dose photon and carbon ion radiation. We have shown for the first time that MSCs are relatively resistant to therapeutic carbon ion radiotherapy. Additionally, this form of radiation did not markedly alter the defining stem cell properties or the expression of established surface markers in MSCs.

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Related in: MedlinePlus

Photon or carbon ion irradiation do not impair the adhesion or migration abilities of MSCs(A) Relative adhesion rates of MSCs up to 24 hours after treatment with 10 Gy photon or 4 Gy carbon ion irradiation. (B) Average velocity of MSCs after irradiation as assessed by time-lapse microscopy. Error bars show standard deviation.
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Figure 2: Photon or carbon ion irradiation do not impair the adhesion or migration abilities of MSCs(A) Relative adhesion rates of MSCs up to 24 hours after treatment with 10 Gy photon or 4 Gy carbon ion irradiation. (B) Average velocity of MSCs after irradiation as assessed by time-lapse microscopy. Error bars show standard deviation.

Mentions: The ability of MSCs to adhere to plastic surfaces was measured up to 24 after treatment with 10 Gy photon or 4 Gy carbon ion irradiation (Figure 2A). Particle-irradiated MSCs were found to exhibit a short delay in the onset of adherence compared to photon- or mock-treated samples. However, the observed small difference in adherence at early time points was abolished at 24 hours after treatment, with highly similar adherence rates above 80% of MSCs for both radiation modalities. Additionally, we did not measure a significant difference between mock-treated and irradiated MSC samples at 24 hours after treatment, suggesting that the ability to adhere is not hampered even by high doses of photon or particle radiotherapy.


Mesenchymal stem cells are resistant to carbon ion radiotherapy.

Nicolay NH, Liang Y, Lopez Perez R, Bostel T, Trinh T, Sisombath S, Weber KJ, Ho AD, Debus J, Saffrich R, Huber PE - Oncotarget (2015)

Photon or carbon ion irradiation do not impair the adhesion or migration abilities of MSCs(A) Relative adhesion rates of MSCs up to 24 hours after treatment with 10 Gy photon or 4 Gy carbon ion irradiation. (B) Average velocity of MSCs after irradiation as assessed by time-lapse microscopy. Error bars show standard deviation.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4385837&req=5

Figure 2: Photon or carbon ion irradiation do not impair the adhesion or migration abilities of MSCs(A) Relative adhesion rates of MSCs up to 24 hours after treatment with 10 Gy photon or 4 Gy carbon ion irradiation. (B) Average velocity of MSCs after irradiation as assessed by time-lapse microscopy. Error bars show standard deviation.
Mentions: The ability of MSCs to adhere to plastic surfaces was measured up to 24 after treatment with 10 Gy photon or 4 Gy carbon ion irradiation (Figure 2A). Particle-irradiated MSCs were found to exhibit a short delay in the onset of adherence compared to photon- or mock-treated samples. However, the observed small difference in adherence at early time points was abolished at 24 hours after treatment, with highly similar adherence rates above 80% of MSCs for both radiation modalities. Additionally, we did not measure a significant difference between mock-treated and irradiated MSC samples at 24 hours after treatment, suggesting that the ability to adhere is not hampered even by high doses of photon or particle radiotherapy.

Bottom Line: Here we show that cellular morphology, adhesion and migration abilities were not measurably altered by photon or carbon ion irradiation.We have shown for the first time that MSCs are relatively resistant to therapeutic carbon ion radiotherapy.Additionally, this form of radiation did not markedly alter the defining stem cell properties or the expression of established surface markers in MSCs.

View Article: PubMed Central - PubMed

Affiliation: Department of Radiation Oncology, Heidelberg University Hospital, Im Neuenheimer Feld, Heidelberg, Germany.

ABSTRACT
Mesenchymal stem cells (MSCs) participate in regeneration of tissues damaged by ionizing radiation. However, radiation can damage MSCs themselves. Here we show that cellular morphology, adhesion and migration abilities were not measurably altered by photon or carbon ion irradiation. The potential for differentiation was unaffected by either form of radiation, and established MSC surface markers were found to be stably expressed irrespective of radiation treatment. MSCs were able to efficiently repair DNA double strand breaks induced by both high-dose photon and carbon ion radiation. We have shown for the first time that MSCs are relatively resistant to therapeutic carbon ion radiotherapy. Additionally, this form of radiation did not markedly alter the defining stem cell properties or the expression of established surface markers in MSCs.

Show MeSH
Related in: MedlinePlus