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Antigenic heterogeneity in granular cell ameloblastoma: an immunohistochemical study.

Ramya R, Sudheerkanth PK, Eapen C, Alex VK, Rajkumar K, Rajashree P - J Int Oral Health (2015)

Bottom Line: S100 was negative in three cases and showed a mild positivity in two cases.Bcl2, p53, vimentin and desmin were negative in all the five cases.This study presents a heterogenous nature of the granular cells; however, further validation is required with a larger sample size.

View Article: PubMed Central - PubMed

Affiliation: Reader, Department of Oral Pathology, SRM Dental College, Chennai, Tamil Nadu, India.

ABSTRACT

Background: Nature of granular cells in granular cell ameloblastoma (GCA) has always invoked considerable interest. The present study aims at antigenic characterization in five such cases with a panel of markers.

Materials and methods: Tissue specimens of five patients with GCA were fixed in buffered formalin and later embedded in paraffin wax. Blocks were sliced into 3 µ thick sections for immunohistochemical analysis using a panel of markers CD68, Bcl2, S100, p53, cytokeratin (AE1/AE3), vimentin and desmin.

Results: All five cases were strongly positive for cytokeratin and CD68. S100 was negative in three cases and showed a mild positivity in two cases. Bcl2, p53, vimentin and desmin were negative in all the five cases.

Conclusions: This study presents a heterogenous nature of the granular cells; however, further validation is required with a larger sample size.

No MeSH data available.


Related in: MedlinePlus

Mild positivity in granular cells with S100 (×40).
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Figure 4: Mild positivity in granular cells with S100 (×40).

Mentions: Negative expression of antiapoptotic factors such as Bcl-2 and p53 proteins in granular cells indicate that there is increased apoptosis in the granular cells. This finding was similar to the report by Kumomoto et al. who reported apoptosis in the granular cells.20 Contradictory to previous reports is the presence of a mild positivity with S100 unlike other previously published reports (Figure 4). S100 is normally present in cells derived from the neural crest chondrocytes, adipocytes, myoepithelial cells, macrophages, langerhans cells, dendritic cells, and keratinocytes. Mild positivity of S100 could be suggestive of transdifferentiation of the cells. Such heterogenous presentation of granular ameloblastomas evokes more interest to further ratify its true nature.


Antigenic heterogeneity in granular cell ameloblastoma: an immunohistochemical study.

Ramya R, Sudheerkanth PK, Eapen C, Alex VK, Rajkumar K, Rajashree P - J Int Oral Health (2015)

Mild positivity in granular cells with S100 (×40).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4385726&req=5

Figure 4: Mild positivity in granular cells with S100 (×40).
Mentions: Negative expression of antiapoptotic factors such as Bcl-2 and p53 proteins in granular cells indicate that there is increased apoptosis in the granular cells. This finding was similar to the report by Kumomoto et al. who reported apoptosis in the granular cells.20 Contradictory to previous reports is the presence of a mild positivity with S100 unlike other previously published reports (Figure 4). S100 is normally present in cells derived from the neural crest chondrocytes, adipocytes, myoepithelial cells, macrophages, langerhans cells, dendritic cells, and keratinocytes. Mild positivity of S100 could be suggestive of transdifferentiation of the cells. Such heterogenous presentation of granular ameloblastomas evokes more interest to further ratify its true nature.

Bottom Line: S100 was negative in three cases and showed a mild positivity in two cases.Bcl2, p53, vimentin and desmin were negative in all the five cases.This study presents a heterogenous nature of the granular cells; however, further validation is required with a larger sample size.

View Article: PubMed Central - PubMed

Affiliation: Reader, Department of Oral Pathology, SRM Dental College, Chennai, Tamil Nadu, India.

ABSTRACT

Background: Nature of granular cells in granular cell ameloblastoma (GCA) has always invoked considerable interest. The present study aims at antigenic characterization in five such cases with a panel of markers.

Materials and methods: Tissue specimens of five patients with GCA were fixed in buffered formalin and later embedded in paraffin wax. Blocks were sliced into 3 µ thick sections for immunohistochemical analysis using a panel of markers CD68, Bcl2, S100, p53, cytokeratin (AE1/AE3), vimentin and desmin.

Results: All five cases were strongly positive for cytokeratin and CD68. S100 was negative in three cases and showed a mild positivity in two cases. Bcl2, p53, vimentin and desmin were negative in all the five cases.

Conclusions: This study presents a heterogenous nature of the granular cells; however, further validation is required with a larger sample size.

No MeSH data available.


Related in: MedlinePlus