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Efficacy of Oral Brush Biopsy without Computer-Assisted Analysis in Oral Premalignant and Malignant Lesions: A Study.

Trakroo A, Sunil MK, Trivedi A, Garg R, Kulkarni A, Arora S - J Int Oral Health (2015)

Bottom Line: Proportions were compared using Chi-square or Fisher's exact test.This technique showed a reasonable sensitivity and specificity thus substantiating its reliability in evaluation of oral premalignant and malignant lesions.It is suitable in population screening programs and for pre- and post-treatment observation of confirmed premalignant and malignant lesions and has proved applications in incapacitated areas.

View Article: PubMed Central - PubMed

Affiliation: Post Graduate Student, Department of Oral Medicine & Radiology, Guru Nanak Dev Dental and Research Institute, Sunam, Punjab, India.

ABSTRACT

Background: In the present study, the reliability of oral brush biopsy in identifying dysplasia in clinically diagnosed oral potentially malignant and malignant lesions was evaluated while comparing the findings with scalpel biopsy in terms of sensitivity and specificity.

Materials and methods: In our study, a total number of 50 patients that included both premalignant and malignant lesions were included. Oral brush cytology using a cytobrush was done for all patients, which was followed by incisional biopsy. Sensitivity, specificity, positive and negative predictive values were obtained. To see the agreement between two modalities Kappa test of agreement was applied. A P < 0.05 was considered to indicate statistical significance. Proportions were compared using Chi-square or Fisher's exact test.

Results: Brush cytology using a cytobrush is a reliable adjunct to histopathology in detecting oral premalignant and malignant oral lesions and can be easily performed with less cost and less discomfort. This technique showed a reasonable sensitivity and specificity thus substantiating its reliability in evaluation of oral premalignant and malignant lesions.

Conclusion: The oral brush biopsy is a simple and rapid, non-invasive and relatively painless and well accepted by patient. It is suitable in population screening programs and for pre- and post-treatment observation of confirmed premalignant and malignant lesions and has proved applications in incapacitated areas.

No MeSH data available.


Related in: MedlinePlus

Cytopatholgy picture showing Class V smear showing loose cohesive clusters of pleomorphic cancer cells with scanty cytoplasm and enlarged nuclei containing numerous nucleoli suggestive of moderately differentiated squamous-cell carcinoma.
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Figure 3: Cytopatholgy picture showing Class V smear showing loose cohesive clusters of pleomorphic cancer cells with scanty cytoplasm and enlarged nuclei containing numerous nucleoli suggestive of moderately differentiated squamous-cell carcinoma.

Mentions: Removed cells were transferred to a glass slide by distributing the obtained material evenly over the glass surface, and smear was made. The slides were then flooded with fixative solution (95% alcohol). The cellular sample on the slide was stained with Hematoxylin and Eosin and Papanicolaou method and scanned by light microscopy. Dysplastic features were looked for cells as the variation in nuclear size and shape (pleomorphism), nuclear borders, nucleo: Cytoplasmic ratio, number of nuclei, binucleation, tadpole forms, keratinization and hyperchromatism, chromatin pattern and distribution as well as discrepancy in nucleo-cytoplasmic maturation and a cytological diagnosis was given (Figures 3 and 4).6


Efficacy of Oral Brush Biopsy without Computer-Assisted Analysis in Oral Premalignant and Malignant Lesions: A Study.

Trakroo A, Sunil MK, Trivedi A, Garg R, Kulkarni A, Arora S - J Int Oral Health (2015)

Cytopatholgy picture showing Class V smear showing loose cohesive clusters of pleomorphic cancer cells with scanty cytoplasm and enlarged nuclei containing numerous nucleoli suggestive of moderately differentiated squamous-cell carcinoma.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4385723&req=5

Figure 3: Cytopatholgy picture showing Class V smear showing loose cohesive clusters of pleomorphic cancer cells with scanty cytoplasm and enlarged nuclei containing numerous nucleoli suggestive of moderately differentiated squamous-cell carcinoma.
Mentions: Removed cells were transferred to a glass slide by distributing the obtained material evenly over the glass surface, and smear was made. The slides were then flooded with fixative solution (95% alcohol). The cellular sample on the slide was stained with Hematoxylin and Eosin and Papanicolaou method and scanned by light microscopy. Dysplastic features were looked for cells as the variation in nuclear size and shape (pleomorphism), nuclear borders, nucleo: Cytoplasmic ratio, number of nuclei, binucleation, tadpole forms, keratinization and hyperchromatism, chromatin pattern and distribution as well as discrepancy in nucleo-cytoplasmic maturation and a cytological diagnosis was given (Figures 3 and 4).6

Bottom Line: Proportions were compared using Chi-square or Fisher's exact test.This technique showed a reasonable sensitivity and specificity thus substantiating its reliability in evaluation of oral premalignant and malignant lesions.It is suitable in population screening programs and for pre- and post-treatment observation of confirmed premalignant and malignant lesions and has proved applications in incapacitated areas.

View Article: PubMed Central - PubMed

Affiliation: Post Graduate Student, Department of Oral Medicine & Radiology, Guru Nanak Dev Dental and Research Institute, Sunam, Punjab, India.

ABSTRACT

Background: In the present study, the reliability of oral brush biopsy in identifying dysplasia in clinically diagnosed oral potentially malignant and malignant lesions was evaluated while comparing the findings with scalpel biopsy in terms of sensitivity and specificity.

Materials and methods: In our study, a total number of 50 patients that included both premalignant and malignant lesions were included. Oral brush cytology using a cytobrush was done for all patients, which was followed by incisional biopsy. Sensitivity, specificity, positive and negative predictive values were obtained. To see the agreement between two modalities Kappa test of agreement was applied. A P < 0.05 was considered to indicate statistical significance. Proportions were compared using Chi-square or Fisher's exact test.

Results: Brush cytology using a cytobrush is a reliable adjunct to histopathology in detecting oral premalignant and malignant oral lesions and can be easily performed with less cost and less discomfort. This technique showed a reasonable sensitivity and specificity thus substantiating its reliability in evaluation of oral premalignant and malignant lesions.

Conclusion: The oral brush biopsy is a simple and rapid, non-invasive and relatively painless and well accepted by patient. It is suitable in population screening programs and for pre- and post-treatment observation of confirmed premalignant and malignant lesions and has proved applications in incapacitated areas.

No MeSH data available.


Related in: MedlinePlus