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Efficacy of Oral Brush Biopsy without Computer-Assisted Analysis in Oral Premalignant and Malignant Lesions: A Study.

Trakroo A, Sunil MK, Trivedi A, Garg R, Kulkarni A, Arora S - J Int Oral Health (2015)

Bottom Line: Sensitivity, specificity, positive and negative predictive values were obtained.A P < 0.05 was considered to indicate statistical significance.Proportions were compared using Chi-square or Fisher's exact test.

View Article: PubMed Central - PubMed

Affiliation: Post Graduate Student, Department of Oral Medicine & Radiology, Guru Nanak Dev Dental and Research Institute, Sunam, Punjab, India.

ABSTRACT

Background: In the present study, the reliability of oral brush biopsy in identifying dysplasia in clinically diagnosed oral potentially malignant and malignant lesions was evaluated while comparing the findings with scalpel biopsy in terms of sensitivity and specificity.

Materials and methods: In our study, a total number of 50 patients that included both premalignant and malignant lesions were included. Oral brush cytology using a cytobrush was done for all patients, which was followed by incisional biopsy. Sensitivity, specificity, positive and negative predictive values were obtained. To see the agreement between two modalities Kappa test of agreement was applied. A P < 0.05 was considered to indicate statistical significance. Proportions were compared using Chi-square or Fisher's exact test.

Results: Brush cytology using a cytobrush is a reliable adjunct to histopathology in detecting oral premalignant and malignant oral lesions and can be easily performed with less cost and less discomfort. This technique showed a reasonable sensitivity and specificity thus substantiating its reliability in evaluation of oral premalignant and malignant lesions.

Conclusion: The oral brush biopsy is a simple and rapid, non-invasive and relatively painless and well accepted by patient. It is suitable in population screening programs and for pre- and post-treatment observation of confirmed premalignant and malignant lesions and has proved applications in incapacitated areas.

No MeSH data available.


Related in: MedlinePlus

Intraoral picture showing non homogenous leukoplakia involving left buccal mucosa.
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Figure 1: Intraoral picture showing non homogenous leukoplakia involving left buccal mucosa.

Mentions: The patients reported to Department of Oral Medicine and Radiology, were selected for study purpose. Patients with suspicious premalignant and malignant lesions were selected irrespective of age and gender. Oral premalignant disorders such as Homogenous leukoplakia, speckled leukoplakia, verrucous leukoplakia, erythroplakia, tobacco pouch keratiosis, erosive lichen planus, oral carcinoma and patients with history of tobacco and related products and alcohol consumption were included in the study. Submucosal lesions, including the hemangioma, mucocele, papilloma, aphthous ulcer, fibroma and medically compromised patients (bleeding disorders, clotting disorders, systemic illness) where incisional biopsy could not be performed were excluded from the study. We conducted our study on 50 patients with clinically diagnosed premalignant and malignant lesions. We obtained a written informed consent from the patients, and every patient was subjected to a complete examination both extra-oral and intraoral. A detailed evaluation of various lesions was done. An oral brush cytology, followed by incisional biopsy was performed for every patient. Patients were made to rinse the oral cavity thoroughly with water, and the lesion was visualized. The brush was repeatedly brushed in one direction over entire lesion many times with moderate pressure until pinpoint bleeding was obtained, and thus obtaining epithelial cells through the full thickness of the epithelium (Figures 1 and 2).


Efficacy of Oral Brush Biopsy without Computer-Assisted Analysis in Oral Premalignant and Malignant Lesions: A Study.

Trakroo A, Sunil MK, Trivedi A, Garg R, Kulkarni A, Arora S - J Int Oral Health (2015)

Intraoral picture showing non homogenous leukoplakia involving left buccal mucosa.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4385723&req=5

Figure 1: Intraoral picture showing non homogenous leukoplakia involving left buccal mucosa.
Mentions: The patients reported to Department of Oral Medicine and Radiology, were selected for study purpose. Patients with suspicious premalignant and malignant lesions were selected irrespective of age and gender. Oral premalignant disorders such as Homogenous leukoplakia, speckled leukoplakia, verrucous leukoplakia, erythroplakia, tobacco pouch keratiosis, erosive lichen planus, oral carcinoma and patients with history of tobacco and related products and alcohol consumption were included in the study. Submucosal lesions, including the hemangioma, mucocele, papilloma, aphthous ulcer, fibroma and medically compromised patients (bleeding disorders, clotting disorders, systemic illness) where incisional biopsy could not be performed were excluded from the study. We conducted our study on 50 patients with clinically diagnosed premalignant and malignant lesions. We obtained a written informed consent from the patients, and every patient was subjected to a complete examination both extra-oral and intraoral. A detailed evaluation of various lesions was done. An oral brush cytology, followed by incisional biopsy was performed for every patient. Patients were made to rinse the oral cavity thoroughly with water, and the lesion was visualized. The brush was repeatedly brushed in one direction over entire lesion many times with moderate pressure until pinpoint bleeding was obtained, and thus obtaining epithelial cells through the full thickness of the epithelium (Figures 1 and 2).

Bottom Line: Sensitivity, specificity, positive and negative predictive values were obtained.A P < 0.05 was considered to indicate statistical significance.Proportions were compared using Chi-square or Fisher's exact test.

View Article: PubMed Central - PubMed

Affiliation: Post Graduate Student, Department of Oral Medicine & Radiology, Guru Nanak Dev Dental and Research Institute, Sunam, Punjab, India.

ABSTRACT

Background: In the present study, the reliability of oral brush biopsy in identifying dysplasia in clinically diagnosed oral potentially malignant and malignant lesions was evaluated while comparing the findings with scalpel biopsy in terms of sensitivity and specificity.

Materials and methods: In our study, a total number of 50 patients that included both premalignant and malignant lesions were included. Oral brush cytology using a cytobrush was done for all patients, which was followed by incisional biopsy. Sensitivity, specificity, positive and negative predictive values were obtained. To see the agreement between two modalities Kappa test of agreement was applied. A P < 0.05 was considered to indicate statistical significance. Proportions were compared using Chi-square or Fisher's exact test.

Results: Brush cytology using a cytobrush is a reliable adjunct to histopathology in detecting oral premalignant and malignant oral lesions and can be easily performed with less cost and less discomfort. This technique showed a reasonable sensitivity and specificity thus substantiating its reliability in evaluation of oral premalignant and malignant lesions.

Conclusion: The oral brush biopsy is a simple and rapid, non-invasive and relatively painless and well accepted by patient. It is suitable in population screening programs and for pre- and post-treatment observation of confirmed premalignant and malignant lesions and has proved applications in incapacitated areas.

No MeSH data available.


Related in: MedlinePlus