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Acute exercise induced mitochondrial H₂O₂ production in mouse skeletal muscle: association with p(66Shc) and FOXO3a signaling and antioxidant enzymes.

Wang P, Li CG, Qi Z, Cui D, Ding S - Oxid Med Cell Longev (2015)

Bottom Line: The activity of mitochondrial catalase was slightly reduced in the 90 min exercise group, but it was significantly higher in groups with 120 and 150 min exercise compared to that of 90 min exercise group.The activity of SOD was not significantly affected.The results indicate that acute exercise increases mitochondrial H2O2 production in the skeletal muscle, which is associated with the upregulation of p(66Shc) and FOXO3a.

View Article: PubMed Central - PubMed

Affiliation: School of Physical Education and Sports Science, Hangzhou Normal University, Hangzhou 311121, China.

ABSTRACT
Exercise induced skeletal muscle phenotype change involves a complex interplay between signaling pathways and downstream regulators. This study aims to investigate the effect of acute exercise on mitochondrial H2O2 production and its association with p(66Shc), FOXO3a, and antioxidant enzymes. Male ICR/CD-1 mice were subjected to an acute exercise. Muscle tissues (gastrocnemius and quadriceps femoris) were taken after exercise to measure mitochondrial H2O2 content, expression of p(66Shc) and FOXO3a, and the activity of antioxidant enzymes. The results showed that acute exercise significantly increased mitochondrial H2O2 content and expressions of p(66Shc) and FOXO3a in a time-dependent manner, with a linear correlation between the increase in H2O2 content and p(66Shc) or FOXO3a expression. The activity of mitochondrial catalase was slightly reduced in the 90 min exercise group, but it was significantly higher in groups with 120 and 150 min exercise compared to that of 90 min exercise group. The activity of SOD was not significantly affected. The results indicate that acute exercise increases mitochondrial H2O2 production in the skeletal muscle, which is associated with the upregulation of p(66Shc) and FOXO3a. The association of p(66Shc) and FOXO3a signaling with exercise induced H2O2 generation may play a role in regulating cellular oxidative stress during acute exercise.

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Related in: MedlinePlus

Correlation between expression of p66Shc and FOXO3a mRNA and mitochondrial H2O2 content (mmol/g protein) in skeletal muscles of exercised ICR/CD.
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Related In: Results  -  Collection


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fig3: Correlation between expression of p66Shc and FOXO3a mRNA and mitochondrial H2O2 content (mmol/g protein) in skeletal muscles of exercised ICR/CD.

Mentions: There was a positive correlation between mitochondrial H2O2 content and expression of p66Shc mRNA (r = 0.4723, P < 0.01, Figure 3) in skeletal muscles of exercised ICR/CD. Similarly, there was a positive correlation between mitochondrial H2O2 content and expression of FOXO3a mRNA (r = 0.5623, P < 0.01, Figure 3) in skeletal muscles of exercised ICR/CD-1 mice.


Acute exercise induced mitochondrial H₂O₂ production in mouse skeletal muscle: association with p(66Shc) and FOXO3a signaling and antioxidant enzymes.

Wang P, Li CG, Qi Z, Cui D, Ding S - Oxid Med Cell Longev (2015)

Correlation between expression of p66Shc and FOXO3a mRNA and mitochondrial H2O2 content (mmol/g protein) in skeletal muscles of exercised ICR/CD.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4385701&req=5

fig3: Correlation between expression of p66Shc and FOXO3a mRNA and mitochondrial H2O2 content (mmol/g protein) in skeletal muscles of exercised ICR/CD.
Mentions: There was a positive correlation between mitochondrial H2O2 content and expression of p66Shc mRNA (r = 0.4723, P < 0.01, Figure 3) in skeletal muscles of exercised ICR/CD. Similarly, there was a positive correlation between mitochondrial H2O2 content and expression of FOXO3a mRNA (r = 0.5623, P < 0.01, Figure 3) in skeletal muscles of exercised ICR/CD-1 mice.

Bottom Line: The activity of mitochondrial catalase was slightly reduced in the 90 min exercise group, but it was significantly higher in groups with 120 and 150 min exercise compared to that of 90 min exercise group.The activity of SOD was not significantly affected.The results indicate that acute exercise increases mitochondrial H2O2 production in the skeletal muscle, which is associated with the upregulation of p(66Shc) and FOXO3a.

View Article: PubMed Central - PubMed

Affiliation: School of Physical Education and Sports Science, Hangzhou Normal University, Hangzhou 311121, China.

ABSTRACT
Exercise induced skeletal muscle phenotype change involves a complex interplay between signaling pathways and downstream regulators. This study aims to investigate the effect of acute exercise on mitochondrial H2O2 production and its association with p(66Shc), FOXO3a, and antioxidant enzymes. Male ICR/CD-1 mice were subjected to an acute exercise. Muscle tissues (gastrocnemius and quadriceps femoris) were taken after exercise to measure mitochondrial H2O2 content, expression of p(66Shc) and FOXO3a, and the activity of antioxidant enzymes. The results showed that acute exercise significantly increased mitochondrial H2O2 content and expressions of p(66Shc) and FOXO3a in a time-dependent manner, with a linear correlation between the increase in H2O2 content and p(66Shc) or FOXO3a expression. The activity of mitochondrial catalase was slightly reduced in the 90 min exercise group, but it was significantly higher in groups with 120 and 150 min exercise compared to that of 90 min exercise group. The activity of SOD was not significantly affected. The results indicate that acute exercise increases mitochondrial H2O2 production in the skeletal muscle, which is associated with the upregulation of p(66Shc) and FOXO3a. The association of p(66Shc) and FOXO3a signaling with exercise induced H2O2 generation may play a role in regulating cellular oxidative stress during acute exercise.

Show MeSH
Related in: MedlinePlus