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Training effects on ROS production determined by electron paramagnetic resonance in master swimmers.

Mrakic-Sposta S, Gussoni M, Porcelli S, Pugliese L, Pavei G, Bellistri G, Montorsi M, Tacchini P, Vezzoli A - Oxid Med Cell Longev (2015)

Bottom Line: A significant (P < 0.01) increase of ROS production from REST to the END of IE in PRE Trg (2.82 ± 0.66 versus 3.28 ± 0.66 µmol·min(-1)) was observed.HIDT increased peak oxygen consumption (36.1 ± 4.3 versus 40.6 ± 5.7 mL·kg(-1)·min(-1) PRE and POST Trg, resp.) and the antioxidant capacity (+13%) while it significantly decreased the ROS production both at REST (-20%) and after IE (-25%).The observed link between ROS production, adaptive antioxidant defense mechanisms, and peak oxygen consumption provides new insight into the correlation between ROS response pathways and muscle metabolic function.

View Article: PubMed Central - PubMed

Affiliation: Istituto di Bioimmagini e di Fisiologia Molecolare, Consiglio Nazionale delle Ricerche, Via Fratelli Cervi 93, 20090 Segrate, Italy.

ABSTRACT
Acute exercise induces an increase in Reactive Oxygen Species (ROS) production dependent on exercise intensity with highest ROS amount generated by strenuous exercise. However, chronic repetition of exercise, that is, exercise training, may reduce exercise-induced oxidative stress. Aim of this study was to evaluate the effects of 6-weeks high-intensity discontinuous training (HIDT), characterized by repeated variations of intensity and changes of redox potential, on ROS production and antioxidant capacity in sixteen master swimmers. Time course changes of ROS generation were assessed by Electron Paramagnetic Resonance in capillary blood by a microinvasive approach. An incremental arm-ergometer exercise (IE) until exhaustion was carried out at both before (PRE) and after (POST) training (Trg) period. A significant (P < 0.01) increase of ROS production from REST to the END of IE in PRE Trg (2.82 ± 0.66 versus 3.28 ± 0.66 µmol·min(-1)) was observed. HIDT increased peak oxygen consumption (36.1 ± 4.3 versus 40.6 ± 5.7 mL·kg(-1)·min(-1) PRE and POST Trg, resp.) and the antioxidant capacity (+13%) while it significantly decreased the ROS production both at REST (-20%) and after IE (-25%). The observed link between ROS production, adaptive antioxidant defense mechanisms, and peak oxygen consumption provides new insight into the correlation between ROS response pathways and muscle metabolic function.

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Related in: MedlinePlus

Sketch of the experimental protocol adopted to measure ROS production rate in swimmers. The data were collected at REST, at the END of the incremental arm-ergometer exercise (IE), carried out up to voluntary exhaustion, and at 10 min of the recovery period (see upper part of the figure) both before (PRE Trg) and after (POST Trg) training (lower part of the figure).
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fig1: Sketch of the experimental protocol adopted to measure ROS production rate in swimmers. The data were collected at REST, at the END of the incremental arm-ergometer exercise (IE), carried out up to voluntary exhaustion, and at 10 min of the recovery period (see upper part of the figure) both before (PRE Trg) and after (POST Trg) training (lower part of the figure).

Mentions: At rest, at the end of IE and after 10 minutes of recovery, ROS production rate was determined in 50 μL capillary blood by means of a recently developed EPR microinvasive method [18, 19]. The capillary blood samples were collected at both PRE and POST Trg periods. The experimental protocol adopted for ROS detection in master swimmers is shown in Figure 1.


Training effects on ROS production determined by electron paramagnetic resonance in master swimmers.

Mrakic-Sposta S, Gussoni M, Porcelli S, Pugliese L, Pavei G, Bellistri G, Montorsi M, Tacchini P, Vezzoli A - Oxid Med Cell Longev (2015)

Sketch of the experimental protocol adopted to measure ROS production rate in swimmers. The data were collected at REST, at the END of the incremental arm-ergometer exercise (IE), carried out up to voluntary exhaustion, and at 10 min of the recovery period (see upper part of the figure) both before (PRE Trg) and after (POST Trg) training (lower part of the figure).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4385700&req=5

fig1: Sketch of the experimental protocol adopted to measure ROS production rate in swimmers. The data were collected at REST, at the END of the incremental arm-ergometer exercise (IE), carried out up to voluntary exhaustion, and at 10 min of the recovery period (see upper part of the figure) both before (PRE Trg) and after (POST Trg) training (lower part of the figure).
Mentions: At rest, at the end of IE and after 10 minutes of recovery, ROS production rate was determined in 50 μL capillary blood by means of a recently developed EPR microinvasive method [18, 19]. The capillary blood samples were collected at both PRE and POST Trg periods. The experimental protocol adopted for ROS detection in master swimmers is shown in Figure 1.

Bottom Line: A significant (P < 0.01) increase of ROS production from REST to the END of IE in PRE Trg (2.82 ± 0.66 versus 3.28 ± 0.66 µmol·min(-1)) was observed.HIDT increased peak oxygen consumption (36.1 ± 4.3 versus 40.6 ± 5.7 mL·kg(-1)·min(-1) PRE and POST Trg, resp.) and the antioxidant capacity (+13%) while it significantly decreased the ROS production both at REST (-20%) and after IE (-25%).The observed link between ROS production, adaptive antioxidant defense mechanisms, and peak oxygen consumption provides new insight into the correlation between ROS response pathways and muscle metabolic function.

View Article: PubMed Central - PubMed

Affiliation: Istituto di Bioimmagini e di Fisiologia Molecolare, Consiglio Nazionale delle Ricerche, Via Fratelli Cervi 93, 20090 Segrate, Italy.

ABSTRACT
Acute exercise induces an increase in Reactive Oxygen Species (ROS) production dependent on exercise intensity with highest ROS amount generated by strenuous exercise. However, chronic repetition of exercise, that is, exercise training, may reduce exercise-induced oxidative stress. Aim of this study was to evaluate the effects of 6-weeks high-intensity discontinuous training (HIDT), characterized by repeated variations of intensity and changes of redox potential, on ROS production and antioxidant capacity in sixteen master swimmers. Time course changes of ROS generation were assessed by Electron Paramagnetic Resonance in capillary blood by a microinvasive approach. An incremental arm-ergometer exercise (IE) until exhaustion was carried out at both before (PRE) and after (POST) training (Trg) period. A significant (P < 0.01) increase of ROS production from REST to the END of IE in PRE Trg (2.82 ± 0.66 versus 3.28 ± 0.66 µmol·min(-1)) was observed. HIDT increased peak oxygen consumption (36.1 ± 4.3 versus 40.6 ± 5.7 mL·kg(-1)·min(-1) PRE and POST Trg, resp.) and the antioxidant capacity (+13%) while it significantly decreased the ROS production both at REST (-20%) and after IE (-25%). The observed link between ROS production, adaptive antioxidant defense mechanisms, and peak oxygen consumption provides new insight into the correlation between ROS response pathways and muscle metabolic function.

Show MeSH
Related in: MedlinePlus