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Cardioprotective effects of voluntary exercise in a rat model: role of matrix metalloproteinase-2.

Pósa A, Szabó R, Kupai K, Baráth Z, Szalai Z, Csonka A, Veszelka M, Gyöngyösi M, Radák Z, Ménesi R, Pávó I, Berkó AM, Varga C - Oxid Med Cell Longev (2015)

Bottom Line: Regular exercise at moderate intensity reduces cardiovascular risks.Voluntary wheel-running exercise decreased both the sera (64 kDa and 72 kDa) and the coronary effluent (64 kDa) MMP-2 level, reduced the development of ST depression, improved the isolated heart perfusion, and decreased the ratio of infarct size. 6 weeks of voluntary exercise training preserved the heart against cardiac injury.This protective mechanism might be associated with the decreased activity of MMP-2.

View Article: PubMed Central - PubMed

Affiliation: Department of Physiology, Anatomy and Neuroscience, University of Szeged, Kozep Fasor 52, Szeged 6726, Hungary.

ABSTRACT

Background: Regular exercise at moderate intensity reduces cardiovascular risks. Matrix metalloproteinases (MMPs) play a major role in cardiac remodeling, facilitating physiological adaptation to exercise. The aim of this study was to examine the influence of voluntary physical exercise on the MMP-2 enzyme activity and to investigate the cardiac performance by measurement of angina susceptibility of the heart, the basal blood pressure, the surviving aorta ring contraction, and the cardiac infarct size after I/R-induced injury.

Methods: Male Wistar rats were divided into control and exercising groups. After a 6-week period, the serum level of MMP-2, basal blood pressure, cardiac angina susceptibility (the ST segment depression provoked by epinephrine and 30 s later phentolamine), AVP-induced heart perfusion and aorta ring contraction, infarct size following 30 min ischemia and 120 min reperfusion, and coronary effluent MMP-2 activity were measured.

Results: Voluntary wheel-running exercise decreased both the sera (64 kDa and 72 kDa) and the coronary effluent (64 kDa) MMP-2 level, reduced the development of ST depression, improved the isolated heart perfusion, and decreased the ratio of infarct size.

Conclusion: 6 weeks of voluntary exercise training preserved the heart against cardiac injury. This protective mechanism might be associated with the decreased activity of MMP-2.

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Related in: MedlinePlus

Serum matrix metalloproteinase-2 activity (64 kDa and 72 kDa MMP-2, expressed as intensity × mm2) in control (■) and exercising (□) rats. Data are means ± SEM, n = 12-13. Statistical significance: ***P < 0.001 as compared with the control group. (a) Serum 64 kDa matrix metalloproteinase-2 activity (64 kDa MMP-2, expressed as intensity × mm2) in control (■) and exercising (□) rats. (b) Serum 72 kDa matrix metalloproteinase-2 activity (72 kDa MMP-2, expressed as intensity × mm2) in control (■) and exercising (□) rats. The representative image presents a zymographic picture.
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fig1: Serum matrix metalloproteinase-2 activity (64 kDa and 72 kDa MMP-2, expressed as intensity × mm2) in control (■) and exercising (□) rats. Data are means ± SEM, n = 12-13. Statistical significance: ***P < 0.001 as compared with the control group. (a) Serum 64 kDa matrix metalloproteinase-2 activity (64 kDa MMP-2, expressed as intensity × mm2) in control (■) and exercising (□) rats. (b) Serum 72 kDa matrix metalloproteinase-2 activity (72 kDa MMP-2, expressed as intensity × mm2) in control (■) and exercising (□) rats. The representative image presents a zymographic picture.

Mentions: Serum samples were collected from the lateral tail vein. We found that the 6-week voluntary physical exercise significantly (P < 0.001) decreased the serum MMP-2 activity (that of 64 kDa MMP-2 from 1002.71 ± 37.50 to 679.73 ± 34.35 intensity × mm2 and that of 72 kDa MMP-2 from 314.93 ± 14.80 to 183.33 ± 7.12 intensity × mm2, n = 12-13). Data are shown in Figure 1.


Cardioprotective effects of voluntary exercise in a rat model: role of matrix metalloproteinase-2.

Pósa A, Szabó R, Kupai K, Baráth Z, Szalai Z, Csonka A, Veszelka M, Gyöngyösi M, Radák Z, Ménesi R, Pávó I, Berkó AM, Varga C - Oxid Med Cell Longev (2015)

Serum matrix metalloproteinase-2 activity (64 kDa and 72 kDa MMP-2, expressed as intensity × mm2) in control (■) and exercising (□) rats. Data are means ± SEM, n = 12-13. Statistical significance: ***P < 0.001 as compared with the control group. (a) Serum 64 kDa matrix metalloproteinase-2 activity (64 kDa MMP-2, expressed as intensity × mm2) in control (■) and exercising (□) rats. (b) Serum 72 kDa matrix metalloproteinase-2 activity (72 kDa MMP-2, expressed as intensity × mm2) in control (■) and exercising (□) rats. The representative image presents a zymographic picture.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4385683&req=5

fig1: Serum matrix metalloproteinase-2 activity (64 kDa and 72 kDa MMP-2, expressed as intensity × mm2) in control (■) and exercising (□) rats. Data are means ± SEM, n = 12-13. Statistical significance: ***P < 0.001 as compared with the control group. (a) Serum 64 kDa matrix metalloproteinase-2 activity (64 kDa MMP-2, expressed as intensity × mm2) in control (■) and exercising (□) rats. (b) Serum 72 kDa matrix metalloproteinase-2 activity (72 kDa MMP-2, expressed as intensity × mm2) in control (■) and exercising (□) rats. The representative image presents a zymographic picture.
Mentions: Serum samples were collected from the lateral tail vein. We found that the 6-week voluntary physical exercise significantly (P < 0.001) decreased the serum MMP-2 activity (that of 64 kDa MMP-2 from 1002.71 ± 37.50 to 679.73 ± 34.35 intensity × mm2 and that of 72 kDa MMP-2 from 314.93 ± 14.80 to 183.33 ± 7.12 intensity × mm2, n = 12-13). Data are shown in Figure 1.

Bottom Line: Regular exercise at moderate intensity reduces cardiovascular risks.Voluntary wheel-running exercise decreased both the sera (64 kDa and 72 kDa) and the coronary effluent (64 kDa) MMP-2 level, reduced the development of ST depression, improved the isolated heart perfusion, and decreased the ratio of infarct size. 6 weeks of voluntary exercise training preserved the heart against cardiac injury.This protective mechanism might be associated with the decreased activity of MMP-2.

View Article: PubMed Central - PubMed

Affiliation: Department of Physiology, Anatomy and Neuroscience, University of Szeged, Kozep Fasor 52, Szeged 6726, Hungary.

ABSTRACT

Background: Regular exercise at moderate intensity reduces cardiovascular risks. Matrix metalloproteinases (MMPs) play a major role in cardiac remodeling, facilitating physiological adaptation to exercise. The aim of this study was to examine the influence of voluntary physical exercise on the MMP-2 enzyme activity and to investigate the cardiac performance by measurement of angina susceptibility of the heart, the basal blood pressure, the surviving aorta ring contraction, and the cardiac infarct size after I/R-induced injury.

Methods: Male Wistar rats were divided into control and exercising groups. After a 6-week period, the serum level of MMP-2, basal blood pressure, cardiac angina susceptibility (the ST segment depression provoked by epinephrine and 30 s later phentolamine), AVP-induced heart perfusion and aorta ring contraction, infarct size following 30 min ischemia and 120 min reperfusion, and coronary effluent MMP-2 activity were measured.

Results: Voluntary wheel-running exercise decreased both the sera (64 kDa and 72 kDa) and the coronary effluent (64 kDa) MMP-2 level, reduced the development of ST depression, improved the isolated heart perfusion, and decreased the ratio of infarct size.

Conclusion: 6 weeks of voluntary exercise training preserved the heart against cardiac injury. This protective mechanism might be associated with the decreased activity of MMP-2.

Show MeSH
Related in: MedlinePlus