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Assessment of eccentric exercise-induced oxidative stress using oxidation-reduction potential markers.

Stagos D, Goutzourelas N, Ntontou AM, Kafantaris I, Deli CK, Poulios A, Jamurtas AZ, Bar-Or D, Kouretas D - Oxid Med Cell Longev (2015)

Bottom Line: The aim of the present study was to investigate the use of static (sORP) and capacity ORP (cORP) oxidation-reduction potential markers as measured by the RedoxSYS Diagnostic System in plasma, for assessing eccentric exercise-induced oxidative stress.Moreover, common redox biomarkers were measured, which were protein carbonyls, thiobarbituric acid-reactive substances, total antioxidant capacity in plasma, and catalase activity and glutathione levels in erythrocytes.However, in 11 participants there was a high increase in cORP after exercise, while in 8 participants there was a high decrease.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry and Biotechnology, University of Thessaly, Ploutonos 26 & Aiolou, 41221 Larissa, Greece.

ABSTRACT
The aim of the present study was to investigate the use of static (sORP) and capacity ORP (cORP) oxidation-reduction potential markers as measured by the RedoxSYS Diagnostic System in plasma, for assessing eccentric exercise-induced oxidative stress. Nineteen volunteers performed eccentric exercise with the knee extensors. Blood was collected before, immediately after exercise, and 24, 48, and 72 h after exercise. Moreover, common redox biomarkers were measured, which were protein carbonyls, thiobarbituric acid-reactive substances, total antioxidant capacity in plasma, and catalase activity and glutathione levels in erythrocytes. When the participants were examined as one group, there were not significant differences in any marker after exercise. However, in 11 participants there was a high increase in cORP after exercise, while in 8 participants there was a high decrease. Thus, the participants were divided in low cORP group exhibiting significant decrease in cORP after exercise and in high cORP group exhibiting significant increase. Moreover, only in the low cORP group there was a significant increase in lipid peroxidation after exercise suggesting induction of oxidative stress. The results suggested that high decreases in cORP values after exercise may indicate induction of oxidative stress by eccentric exercise, while high increases in cORP values after exercise may indicate no existence of oxidative stress.

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Related in: MedlinePlus

Percent change in redox biomarker levels of each individual at 24 h, 48 h, and 72 h after eccentric exercise. (a) TAC: total antioxidant capacity (in plasma); (b) GSH: reduced glutathione (in erythrocytes); (c) CARB: protein carbonyl levels (in plasma); (d) TBARS: thiobarbituric acid-reactive substances (in plasma); (e) CAT: catalase activity (in erythrocytes).
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fig1: Percent change in redox biomarker levels of each individual at 24 h, 48 h, and 72 h after eccentric exercise. (a) TAC: total antioxidant capacity (in plasma); (b) GSH: reduced glutathione (in erythrocytes); (c) CARB: protein carbonyl levels (in plasma); (d) TBARS: thiobarbituric acid-reactive substances (in plasma); (e) CAT: catalase activity (in erythrocytes).

Mentions: None of the tested redox markers changed statistically significant postexercise compared to preexercise when all the participants were examined as one group (Table 2). This lack of significance regarding the changes of the oxidative stress markers after exercise can be explained by the great variation that each of them presents between different individuals (Table 2). Moreover, the redox markers in many of the participants in the study changed unexpectedly. For example, in several individuals, TBARS and CARB levels were decreased, while GSH levels were increased after exercise (Figure 1), while according to previous studies the opposite effect was expected [12, 13, 15, 17]. Thus, in all redox markers the participants could be divided into two groups, those with high increase and those with large decrease in the values of the markers after exercise compared to before exercise (Figures 1 and 2). Similar great variation and unexpected findings have also been exhibited in other studies on eccentric exercise [12]. Specifically, Margaritelis et al. [12] have reported that eccentric exercise can induce even reductive stress or negligible stress in a considerable number of people. This great variation in the response of different individuals to eccentric exercise-induced oxidative stress may be attributed to the high complexity of the regulation of redox homeostasis in humans. That is, many different factors such as genetic, physiological, biochemical, and dietary factors can affect the final outcome of oxidant stimuli [28–31].


Assessment of eccentric exercise-induced oxidative stress using oxidation-reduction potential markers.

Stagos D, Goutzourelas N, Ntontou AM, Kafantaris I, Deli CK, Poulios A, Jamurtas AZ, Bar-Or D, Kouretas D - Oxid Med Cell Longev (2015)

Percent change in redox biomarker levels of each individual at 24 h, 48 h, and 72 h after eccentric exercise. (a) TAC: total antioxidant capacity (in plasma); (b) GSH: reduced glutathione (in erythrocytes); (c) CARB: protein carbonyl levels (in plasma); (d) TBARS: thiobarbituric acid-reactive substances (in plasma); (e) CAT: catalase activity (in erythrocytes).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4385679&req=5

fig1: Percent change in redox biomarker levels of each individual at 24 h, 48 h, and 72 h after eccentric exercise. (a) TAC: total antioxidant capacity (in plasma); (b) GSH: reduced glutathione (in erythrocytes); (c) CARB: protein carbonyl levels (in plasma); (d) TBARS: thiobarbituric acid-reactive substances (in plasma); (e) CAT: catalase activity (in erythrocytes).
Mentions: None of the tested redox markers changed statistically significant postexercise compared to preexercise when all the participants were examined as one group (Table 2). This lack of significance regarding the changes of the oxidative stress markers after exercise can be explained by the great variation that each of them presents between different individuals (Table 2). Moreover, the redox markers in many of the participants in the study changed unexpectedly. For example, in several individuals, TBARS and CARB levels were decreased, while GSH levels were increased after exercise (Figure 1), while according to previous studies the opposite effect was expected [12, 13, 15, 17]. Thus, in all redox markers the participants could be divided into two groups, those with high increase and those with large decrease in the values of the markers after exercise compared to before exercise (Figures 1 and 2). Similar great variation and unexpected findings have also been exhibited in other studies on eccentric exercise [12]. Specifically, Margaritelis et al. [12] have reported that eccentric exercise can induce even reductive stress or negligible stress in a considerable number of people. This great variation in the response of different individuals to eccentric exercise-induced oxidative stress may be attributed to the high complexity of the regulation of redox homeostasis in humans. That is, many different factors such as genetic, physiological, biochemical, and dietary factors can affect the final outcome of oxidant stimuli [28–31].

Bottom Line: The aim of the present study was to investigate the use of static (sORP) and capacity ORP (cORP) oxidation-reduction potential markers as measured by the RedoxSYS Diagnostic System in plasma, for assessing eccentric exercise-induced oxidative stress.Moreover, common redox biomarkers were measured, which were protein carbonyls, thiobarbituric acid-reactive substances, total antioxidant capacity in plasma, and catalase activity and glutathione levels in erythrocytes.However, in 11 participants there was a high increase in cORP after exercise, while in 8 participants there was a high decrease.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry and Biotechnology, University of Thessaly, Ploutonos 26 & Aiolou, 41221 Larissa, Greece.

ABSTRACT
The aim of the present study was to investigate the use of static (sORP) and capacity ORP (cORP) oxidation-reduction potential markers as measured by the RedoxSYS Diagnostic System in plasma, for assessing eccentric exercise-induced oxidative stress. Nineteen volunteers performed eccentric exercise with the knee extensors. Blood was collected before, immediately after exercise, and 24, 48, and 72 h after exercise. Moreover, common redox biomarkers were measured, which were protein carbonyls, thiobarbituric acid-reactive substances, total antioxidant capacity in plasma, and catalase activity and glutathione levels in erythrocytes. When the participants were examined as one group, there were not significant differences in any marker after exercise. However, in 11 participants there was a high increase in cORP after exercise, while in 8 participants there was a high decrease. Thus, the participants were divided in low cORP group exhibiting significant decrease in cORP after exercise and in high cORP group exhibiting significant increase. Moreover, only in the low cORP group there was a significant increase in lipid peroxidation after exercise suggesting induction of oxidative stress. The results suggested that high decreases in cORP values after exercise may indicate induction of oxidative stress by eccentric exercise, while high increases in cORP values after exercise may indicate no existence of oxidative stress.

Show MeSH
Related in: MedlinePlus