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Antifungal and antibacterial metabolites from a French poplar type propolis.

Boisard S, Le Ray AM, Landreau A, Kempf M, Cassisa V, Flurin C, Richomme P - Evid Based Complement Alternat Med (2015)

Bottom Line: Organic extracts showed a significant antifungal activity against C. albicans and C. glabrata (MIC80 between 16 and 31 µg/mL) but only a weak activity towards A. fumigatus (MIC80 = 250 µg/mL).DCM based extracts exhibited a selective Gram-positive antibacterial activity, especially against S. aureus (SA) and several of its methicillin-resistant (MRSA) and methicillin-susceptible (MSSA) strains (MIC100 30-97 µg/mL).A new and active derivative of catechin was also identified whereas a synergistic antimicrobial effect was noticed during this study.

View Article: PubMed Central - PubMed

Affiliation: EA 921 SONAS/SFR 4207 QUASAV, Université d'Angers, 16 boulevard Daviers, 49045 Angers Cedex 01, France.

ABSTRACT
During this study, the in vitro antifungal and antibacterial activities of different extracts (aqueous and organic) obtained from a French propolis batch were evaluated. Antifungal activity was evaluated by broth microdilution on three pathogenic strains: Candida albicans, C. glabrata, and Aspergillus fumigatus. Antibacterial activity was assayed using agar dilution method on 36 Gram-negative and Gram-positive strains including Staphylococcus aureus. Organic extracts showed a significant antifungal activity against C. albicans and C. glabrata (MIC80 between 16 and 31 µg/mL) but only a weak activity towards A. fumigatus (MIC80 = 250 µg/mL). DCM based extracts exhibited a selective Gram-positive antibacterial activity, especially against S. aureus (SA) and several of its methicillin-resistant (MRSA) and methicillin-susceptible (MSSA) strains (MIC100 30-97 µg/mL). A new and active derivative of catechin was also identified whereas a synergistic antimicrobial effect was noticed during this study.

No MeSH data available.


Related in: MedlinePlus

HPLC chromatograms of E5: 1 3,4-dihydroxybenzaldehyde, 2 4-hydroxybenzoic acid, 3 caffeic acid, 4 vanillin, 5 4-hydroxyacetophenone, 6 p-coumaric acid, 7 ferulic acid, 8 isoferulic acid, 9 benzoic acid, 10 3,4-dimethoxycinnamic acid, 11 3-phenylpropanoic acid, 12 pinobanksin-5-methyl ether, 13 cinnamic acid, 14 4-methoxycinnamic acid, 15 pinobanksin, 16 naringenin, 17 quercetin, 18 quercetin-3-methyl ether, 19 pinocembrin-5-methyl ether, 20 1,3-di-p-coumaroylglycerol, 21 1-p-coumaroyl-3-feruloylglycerol, 22 kaempferol, 23 apigenin, 24 cinnamylidene acetic acid, 25 pinocembrin, 26 benzyl caffeate, 27 isopent-3-enyl caffeate, 28 pinobanksin-3-acetate, 29 prenyl caffeate, 30 2-acetyl-1,3-dicoumaroylglycerol, 31 phenylethyl caffeate (CAPE), 32 chrysin, 33 benzyl p-coumarate, 34 galangin, 35 benzyl ferulate, 36 prenyl ferulate, 37 kaempferide, 38 rhamnocitrin, 39 cinnamyl caffeate, 40 8-[(E)-phenylprop-2-en-1-one]-5-methoxy-(±)-catechin (new), 41 cinnamyl isoferulate, 42 cinnamyl p-coumarate, 43 pinostrobin, 44 alpinone-3-acetate, 45 tectochrysin, 46 benzyl cinnamate, 47 cinnamyl benzoate, 48 cinnamyl cinnamate, and 49 cinnamyl cinnamylidene acetate.
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Related In: Results  -  Collection


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fig1: HPLC chromatograms of E5: 1 3,4-dihydroxybenzaldehyde, 2 4-hydroxybenzoic acid, 3 caffeic acid, 4 vanillin, 5 4-hydroxyacetophenone, 6 p-coumaric acid, 7 ferulic acid, 8 isoferulic acid, 9 benzoic acid, 10 3,4-dimethoxycinnamic acid, 11 3-phenylpropanoic acid, 12 pinobanksin-5-methyl ether, 13 cinnamic acid, 14 4-methoxycinnamic acid, 15 pinobanksin, 16 naringenin, 17 quercetin, 18 quercetin-3-methyl ether, 19 pinocembrin-5-methyl ether, 20 1,3-di-p-coumaroylglycerol, 21 1-p-coumaroyl-3-feruloylglycerol, 22 kaempferol, 23 apigenin, 24 cinnamylidene acetic acid, 25 pinocembrin, 26 benzyl caffeate, 27 isopent-3-enyl caffeate, 28 pinobanksin-3-acetate, 29 prenyl caffeate, 30 2-acetyl-1,3-dicoumaroylglycerol, 31 phenylethyl caffeate (CAPE), 32 chrysin, 33 benzyl p-coumarate, 34 galangin, 35 benzyl ferulate, 36 prenyl ferulate, 37 kaempferide, 38 rhamnocitrin, 39 cinnamyl caffeate, 40 8-[(E)-phenylprop-2-en-1-one]-5-methoxy-(±)-catechin (new), 41 cinnamyl isoferulate, 42 cinnamyl p-coumarate, 43 pinostrobin, 44 alpinone-3-acetate, 45 tectochrysin, 46 benzyl cinnamate, 47 cinnamyl benzoate, 48 cinnamyl cinnamate, and 49 cinnamyl cinnamylidene acetate.

Mentions: Figure 1 shows the HPLC chromatogram of the DCM extract E5. 48 compounds were identified by comparison with the literature data (UV/MS) and pure standards or, when needed, through 1H and 13C (1D and 2D) NMR analysis after compound isolation.


Antifungal and antibacterial metabolites from a French poplar type propolis.

Boisard S, Le Ray AM, Landreau A, Kempf M, Cassisa V, Flurin C, Richomme P - Evid Based Complement Alternat Med (2015)

HPLC chromatograms of E5: 1 3,4-dihydroxybenzaldehyde, 2 4-hydroxybenzoic acid, 3 caffeic acid, 4 vanillin, 5 4-hydroxyacetophenone, 6 p-coumaric acid, 7 ferulic acid, 8 isoferulic acid, 9 benzoic acid, 10 3,4-dimethoxycinnamic acid, 11 3-phenylpropanoic acid, 12 pinobanksin-5-methyl ether, 13 cinnamic acid, 14 4-methoxycinnamic acid, 15 pinobanksin, 16 naringenin, 17 quercetin, 18 quercetin-3-methyl ether, 19 pinocembrin-5-methyl ether, 20 1,3-di-p-coumaroylglycerol, 21 1-p-coumaroyl-3-feruloylglycerol, 22 kaempferol, 23 apigenin, 24 cinnamylidene acetic acid, 25 pinocembrin, 26 benzyl caffeate, 27 isopent-3-enyl caffeate, 28 pinobanksin-3-acetate, 29 prenyl caffeate, 30 2-acetyl-1,3-dicoumaroylglycerol, 31 phenylethyl caffeate (CAPE), 32 chrysin, 33 benzyl p-coumarate, 34 galangin, 35 benzyl ferulate, 36 prenyl ferulate, 37 kaempferide, 38 rhamnocitrin, 39 cinnamyl caffeate, 40 8-[(E)-phenylprop-2-en-1-one]-5-methoxy-(±)-catechin (new), 41 cinnamyl isoferulate, 42 cinnamyl p-coumarate, 43 pinostrobin, 44 alpinone-3-acetate, 45 tectochrysin, 46 benzyl cinnamate, 47 cinnamyl benzoate, 48 cinnamyl cinnamate, and 49 cinnamyl cinnamylidene acetate.
© Copyright Policy - open-access
Related In: Results  -  Collection

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fig1: HPLC chromatograms of E5: 1 3,4-dihydroxybenzaldehyde, 2 4-hydroxybenzoic acid, 3 caffeic acid, 4 vanillin, 5 4-hydroxyacetophenone, 6 p-coumaric acid, 7 ferulic acid, 8 isoferulic acid, 9 benzoic acid, 10 3,4-dimethoxycinnamic acid, 11 3-phenylpropanoic acid, 12 pinobanksin-5-methyl ether, 13 cinnamic acid, 14 4-methoxycinnamic acid, 15 pinobanksin, 16 naringenin, 17 quercetin, 18 quercetin-3-methyl ether, 19 pinocembrin-5-methyl ether, 20 1,3-di-p-coumaroylglycerol, 21 1-p-coumaroyl-3-feruloylglycerol, 22 kaempferol, 23 apigenin, 24 cinnamylidene acetic acid, 25 pinocembrin, 26 benzyl caffeate, 27 isopent-3-enyl caffeate, 28 pinobanksin-3-acetate, 29 prenyl caffeate, 30 2-acetyl-1,3-dicoumaroylglycerol, 31 phenylethyl caffeate (CAPE), 32 chrysin, 33 benzyl p-coumarate, 34 galangin, 35 benzyl ferulate, 36 prenyl ferulate, 37 kaempferide, 38 rhamnocitrin, 39 cinnamyl caffeate, 40 8-[(E)-phenylprop-2-en-1-one]-5-methoxy-(±)-catechin (new), 41 cinnamyl isoferulate, 42 cinnamyl p-coumarate, 43 pinostrobin, 44 alpinone-3-acetate, 45 tectochrysin, 46 benzyl cinnamate, 47 cinnamyl benzoate, 48 cinnamyl cinnamate, and 49 cinnamyl cinnamylidene acetate.
Mentions: Figure 1 shows the HPLC chromatogram of the DCM extract E5. 48 compounds were identified by comparison with the literature data (UV/MS) and pure standards or, when needed, through 1H and 13C (1D and 2D) NMR analysis after compound isolation.

Bottom Line: Organic extracts showed a significant antifungal activity against C. albicans and C. glabrata (MIC80 between 16 and 31 µg/mL) but only a weak activity towards A. fumigatus (MIC80 = 250 µg/mL).DCM based extracts exhibited a selective Gram-positive antibacterial activity, especially against S. aureus (SA) and several of its methicillin-resistant (MRSA) and methicillin-susceptible (MSSA) strains (MIC100 30-97 µg/mL).A new and active derivative of catechin was also identified whereas a synergistic antimicrobial effect was noticed during this study.

View Article: PubMed Central - PubMed

Affiliation: EA 921 SONAS/SFR 4207 QUASAV, Université d'Angers, 16 boulevard Daviers, 49045 Angers Cedex 01, France.

ABSTRACT
During this study, the in vitro antifungal and antibacterial activities of different extracts (aqueous and organic) obtained from a French propolis batch were evaluated. Antifungal activity was evaluated by broth microdilution on three pathogenic strains: Candida albicans, C. glabrata, and Aspergillus fumigatus. Antibacterial activity was assayed using agar dilution method on 36 Gram-negative and Gram-positive strains including Staphylococcus aureus. Organic extracts showed a significant antifungal activity against C. albicans and C. glabrata (MIC80 between 16 and 31 µg/mL) but only a weak activity towards A. fumigatus (MIC80 = 250 µg/mL). DCM based extracts exhibited a selective Gram-positive antibacterial activity, especially against S. aureus (SA) and several of its methicillin-resistant (MRSA) and methicillin-susceptible (MSSA) strains (MIC100 30-97 µg/mL). A new and active derivative of catechin was also identified whereas a synergistic antimicrobial effect was noticed during this study.

No MeSH data available.


Related in: MedlinePlus