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Production and characterization of biodiesel using nonedible castor oil by immobilized lipase from Bacillus aerius.

Narwal SK, Saun NK, Dogra P, Chauhan G, Gupta R - Biomed Res Int (2015)

Bottom Line: A novel thermotolerant lipase from Bacillus aerius was immobilized on inexpensive silica gel matrix.The immobilized lipase was used for the synthesis of biodiesel using castor oil as a substrate in a solvent free system at 55°C under shaking in a chemical reactor.Several crucial parameters affecting biodiesel yield such as incubation time, temperature, substrate molar ratio, and amount of lipase were optimized.

View Article: PubMed Central - PubMed

Affiliation: Department of Biotechnology, Himachal Pradesh University, Summer Hill, Shimla 171005, India.

ABSTRACT
A novel thermotolerant lipase from Bacillus aerius was immobilized on inexpensive silica gel matrix. The immobilized lipase was used for the synthesis of biodiesel using castor oil as a substrate in a solvent free system at 55°C under shaking in a chemical reactor. Several crucial parameters affecting biodiesel yield such as incubation time, temperature, substrate molar ratio, and amount of lipase were optimized. Under the optimized conditions, the highest biodiesel yield was up to 78.13%. The characterization of synthesized biodiesel was done through FTIR spectroscopy, (1)H NMR spectra, and gas chromatography.

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NMR spectrum of transesterified castor oil.
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fig6: NMR spectrum of transesterified castor oil.

Mentions: The Characterization of ester was also done by various analytical/spectroscopic methods which are FTIR, 1H NMR spectroscopy (Table 1) and gas chromatography. The FTIR spectrum of methyl ricinoleate shows the peak at 1743.39 cm−1 which is due to (–C=O stretching of ester), at 1648 cm−1 due to the (–C=C– stretching) but it has high intensity as the presence of ester group resulted in electronegativity of the neighboring groups and a peak at value 2942 cm−1 (–C–H– stretching) clearly confirms the formation of biodiesel (Figure 5). But when the above spectra were compared with their precursors in literature it was clearly found that the peak at value 1680 cm−1 is absent in Figure 5 which was due to –COOH group clearly confirming that esterification took place at this position and all other peaks remained; only the intensity of the peaks changed because of some new functional groups added in it. The 1H NMR spectra of methyl ester are shown in Figure 6. Signals at values 2.32 ppm and 4.21 ppm were due to presence of –CH2–O–C–O and –C=O functional groups of ester bond which were absent in the spectrum of their precursor. Also in the precursor molecule a signal at value 12.05 ppm is due to –OH group but in its ester form this signal is absent clearly confirming that the reaction took place at this position. All other peaks remained the same. The spectra of the products formed at various steps were matched with the ChemDraw Ultra 10 which unambiguously confirms the formation of biodiesel and also compared with its precursor molecules. From the % yield method and GLC analysis, it was observed that the yield of biodiesel produced was approximately the same.


Production and characterization of biodiesel using nonedible castor oil by immobilized lipase from Bacillus aerius.

Narwal SK, Saun NK, Dogra P, Chauhan G, Gupta R - Biomed Res Int (2015)

NMR spectrum of transesterified castor oil.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4385648&req=5

fig6: NMR spectrum of transesterified castor oil.
Mentions: The Characterization of ester was also done by various analytical/spectroscopic methods which are FTIR, 1H NMR spectroscopy (Table 1) and gas chromatography. The FTIR spectrum of methyl ricinoleate shows the peak at 1743.39 cm−1 which is due to (–C=O stretching of ester), at 1648 cm−1 due to the (–C=C– stretching) but it has high intensity as the presence of ester group resulted in electronegativity of the neighboring groups and a peak at value 2942 cm−1 (–C–H– stretching) clearly confirms the formation of biodiesel (Figure 5). But when the above spectra were compared with their precursors in literature it was clearly found that the peak at value 1680 cm−1 is absent in Figure 5 which was due to –COOH group clearly confirming that esterification took place at this position and all other peaks remained; only the intensity of the peaks changed because of some new functional groups added in it. The 1H NMR spectra of methyl ester are shown in Figure 6. Signals at values 2.32 ppm and 4.21 ppm were due to presence of –CH2–O–C–O and –C=O functional groups of ester bond which were absent in the spectrum of their precursor. Also in the precursor molecule a signal at value 12.05 ppm is due to –OH group but in its ester form this signal is absent clearly confirming that the reaction took place at this position. All other peaks remained the same. The spectra of the products formed at various steps were matched with the ChemDraw Ultra 10 which unambiguously confirms the formation of biodiesel and also compared with its precursor molecules. From the % yield method and GLC analysis, it was observed that the yield of biodiesel produced was approximately the same.

Bottom Line: A novel thermotolerant lipase from Bacillus aerius was immobilized on inexpensive silica gel matrix.The immobilized lipase was used for the synthesis of biodiesel using castor oil as a substrate in a solvent free system at 55°C under shaking in a chemical reactor.Several crucial parameters affecting biodiesel yield such as incubation time, temperature, substrate molar ratio, and amount of lipase were optimized.

View Article: PubMed Central - PubMed

Affiliation: Department of Biotechnology, Himachal Pradesh University, Summer Hill, Shimla 171005, India.

ABSTRACT
A novel thermotolerant lipase from Bacillus aerius was immobilized on inexpensive silica gel matrix. The immobilized lipase was used for the synthesis of biodiesel using castor oil as a substrate in a solvent free system at 55°C under shaking in a chemical reactor. Several crucial parameters affecting biodiesel yield such as incubation time, temperature, substrate molar ratio, and amount of lipase were optimized. Under the optimized conditions, the highest biodiesel yield was up to 78.13%. The characterization of synthesized biodiesel was done through FTIR spectroscopy, (1)H NMR spectra, and gas chromatography.

Show MeSH
Related in: MedlinePlus