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Production and characterization of biodiesel using nonedible castor oil by immobilized lipase from Bacillus aerius.

Narwal SK, Saun NK, Dogra P, Chauhan G, Gupta R - Biomed Res Int (2015)

Bottom Line: The immobilized lipase was used for the synthesis of biodiesel using castor oil as a substrate in a solvent free system at 55°C under shaking in a chemical reactor.Under the optimized conditions, the highest biodiesel yield was up to 78.13%.The characterization of synthesized biodiesel was done through FTIR spectroscopy, (1)H NMR spectra, and gas chromatography.

View Article: PubMed Central - PubMed

Affiliation: Department of Biotechnology, Himachal Pradesh University, Summer Hill, Shimla 171005, India.

ABSTRACT
A novel thermotolerant lipase from Bacillus aerius was immobilized on inexpensive silica gel matrix. The immobilized lipase was used for the synthesis of biodiesel using castor oil as a substrate in a solvent free system at 55°C under shaking in a chemical reactor. Several crucial parameters affecting biodiesel yield such as incubation time, temperature, substrate molar ratio, and amount of lipase were optimized. Under the optimized conditions, the highest biodiesel yield was up to 78.13%. The characterization of synthesized biodiesel was done through FTIR spectroscopy, (1)H NMR spectra, and gas chromatography.

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The effect of methanol/oil molar ratio on the methanolysis of castor oil. Reaction conditions: reaction temperature 55°C, 2% lipase by oil weight, and reaction time 96 h.
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fig3: The effect of methanol/oil molar ratio on the methanolysis of castor oil. Reaction conditions: reaction temperature 55°C, 2% lipase by oil weight, and reaction time 96 h.

Mentions: One of the most important variables affecting the yield of ester is the molar ratio of methanol to oil. A set of experiments was performed in which the oil/methanol molar ratio was varied in the range 1 : 1–1 : 5 (mol/mol). The results obtained (Figure 3) showed that the methyl ester yield (64.08%) was highest at 1 : 4 oil/methanol molar ratio. However further increase in molar ratio beyond the optimal level led to decrease in biodiesel yield. This might be due to the deactivation of the lipase by exposure to methanol. In a recent study, the 1 : 4 substrate molar ratio was found to be the best for the maximum yield of biodiesel [17]. Previously 6 : 20 (oil : methanol) molar ratio was found to be optimum for the synthesis of biodiesel [18].


Production and characterization of biodiesel using nonedible castor oil by immobilized lipase from Bacillus aerius.

Narwal SK, Saun NK, Dogra P, Chauhan G, Gupta R - Biomed Res Int (2015)

The effect of methanol/oil molar ratio on the methanolysis of castor oil. Reaction conditions: reaction temperature 55°C, 2% lipase by oil weight, and reaction time 96 h.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4385648&req=5

fig3: The effect of methanol/oil molar ratio on the methanolysis of castor oil. Reaction conditions: reaction temperature 55°C, 2% lipase by oil weight, and reaction time 96 h.
Mentions: One of the most important variables affecting the yield of ester is the molar ratio of methanol to oil. A set of experiments was performed in which the oil/methanol molar ratio was varied in the range 1 : 1–1 : 5 (mol/mol). The results obtained (Figure 3) showed that the methyl ester yield (64.08%) was highest at 1 : 4 oil/methanol molar ratio. However further increase in molar ratio beyond the optimal level led to decrease in biodiesel yield. This might be due to the deactivation of the lipase by exposure to methanol. In a recent study, the 1 : 4 substrate molar ratio was found to be the best for the maximum yield of biodiesel [17]. Previously 6 : 20 (oil : methanol) molar ratio was found to be optimum for the synthesis of biodiesel [18].

Bottom Line: The immobilized lipase was used for the synthesis of biodiesel using castor oil as a substrate in a solvent free system at 55°C under shaking in a chemical reactor.Under the optimized conditions, the highest biodiesel yield was up to 78.13%.The characterization of synthesized biodiesel was done through FTIR spectroscopy, (1)H NMR spectra, and gas chromatography.

View Article: PubMed Central - PubMed

Affiliation: Department of Biotechnology, Himachal Pradesh University, Summer Hill, Shimla 171005, India.

ABSTRACT
A novel thermotolerant lipase from Bacillus aerius was immobilized on inexpensive silica gel matrix. The immobilized lipase was used for the synthesis of biodiesel using castor oil as a substrate in a solvent free system at 55°C under shaking in a chemical reactor. Several crucial parameters affecting biodiesel yield such as incubation time, temperature, substrate molar ratio, and amount of lipase were optimized. Under the optimized conditions, the highest biodiesel yield was up to 78.13%. The characterization of synthesized biodiesel was done through FTIR spectroscopy, (1)H NMR spectra, and gas chromatography.

Show MeSH
Related in: MedlinePlus