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Production and characterization of biodiesel using nonedible castor oil by immobilized lipase from Bacillus aerius.

Narwal SK, Saun NK, Dogra P, Chauhan G, Gupta R - Biomed Res Int (2015)

Bottom Line: A novel thermotolerant lipase from Bacillus aerius was immobilized on inexpensive silica gel matrix.The immobilized lipase was used for the synthesis of biodiesel using castor oil as a substrate in a solvent free system at 55°C under shaking in a chemical reactor.Several crucial parameters affecting biodiesel yield such as incubation time, temperature, substrate molar ratio, and amount of lipase were optimized.

View Article: PubMed Central - PubMed

Affiliation: Department of Biotechnology, Himachal Pradesh University, Summer Hill, Shimla 171005, India.

ABSTRACT
A novel thermotolerant lipase from Bacillus aerius was immobilized on inexpensive silica gel matrix. The immobilized lipase was used for the synthesis of biodiesel using castor oil as a substrate in a solvent free system at 55°C under shaking in a chemical reactor. Several crucial parameters affecting biodiesel yield such as incubation time, temperature, substrate molar ratio, and amount of lipase were optimized. Under the optimized conditions, the highest biodiesel yield was up to 78.13%. The characterization of synthesized biodiesel was done through FTIR spectroscopy, (1)H NMR spectra, and gas chromatography.

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Effect of temperature on the synthesis of biodiesel. Reaction conditions: reaction time 96 h, methanol/oil 1 : 1 (mol/mol) and 2% lipase by oil weight.
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fig2: Effect of temperature on the synthesis of biodiesel. Reaction conditions: reaction time 96 h, methanol/oil 1 : 1 (mol/mol) and 2% lipase by oil weight.

Mentions: Changes in the reaction temperature can affect the activity and stability of the enzyme and thus the rate of reaction. The effect of temperature on the lipase activity was examined using a temperature range of 40°C to 60°C as shown in Figure 2 with castor oil (1 M) and methanol (1 M) in a solvent free system for 96 h. The maximum biodiesel synthesis (54.08%) was observed at 55°C (Figure 2) under the above reaction conditions. Previously the optimum reaction temperature of 60°C was observed for the synthesis of biodiesel [16]. In a recent study, the optimum temperature of 37°C was observed for the synthesis of biodiesel [17], so this shows mesophilic nature of enzyme but the lipase from Bacillus aerius was thermotolerant; therefore maximum biodiesel production was observed at 55°C.


Production and characterization of biodiesel using nonedible castor oil by immobilized lipase from Bacillus aerius.

Narwal SK, Saun NK, Dogra P, Chauhan G, Gupta R - Biomed Res Int (2015)

Effect of temperature on the synthesis of biodiesel. Reaction conditions: reaction time 96 h, methanol/oil 1 : 1 (mol/mol) and 2% lipase by oil weight.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4385648&req=5

fig2: Effect of temperature on the synthesis of biodiesel. Reaction conditions: reaction time 96 h, methanol/oil 1 : 1 (mol/mol) and 2% lipase by oil weight.
Mentions: Changes in the reaction temperature can affect the activity and stability of the enzyme and thus the rate of reaction. The effect of temperature on the lipase activity was examined using a temperature range of 40°C to 60°C as shown in Figure 2 with castor oil (1 M) and methanol (1 M) in a solvent free system for 96 h. The maximum biodiesel synthesis (54.08%) was observed at 55°C (Figure 2) under the above reaction conditions. Previously the optimum reaction temperature of 60°C was observed for the synthesis of biodiesel [16]. In a recent study, the optimum temperature of 37°C was observed for the synthesis of biodiesel [17], so this shows mesophilic nature of enzyme but the lipase from Bacillus aerius was thermotolerant; therefore maximum biodiesel production was observed at 55°C.

Bottom Line: A novel thermotolerant lipase from Bacillus aerius was immobilized on inexpensive silica gel matrix.The immobilized lipase was used for the synthesis of biodiesel using castor oil as a substrate in a solvent free system at 55°C under shaking in a chemical reactor.Several crucial parameters affecting biodiesel yield such as incubation time, temperature, substrate molar ratio, and amount of lipase were optimized.

View Article: PubMed Central - PubMed

Affiliation: Department of Biotechnology, Himachal Pradesh University, Summer Hill, Shimla 171005, India.

ABSTRACT
A novel thermotolerant lipase from Bacillus aerius was immobilized on inexpensive silica gel matrix. The immobilized lipase was used for the synthesis of biodiesel using castor oil as a substrate in a solvent free system at 55°C under shaking in a chemical reactor. Several crucial parameters affecting biodiesel yield such as incubation time, temperature, substrate molar ratio, and amount of lipase were optimized. Under the optimized conditions, the highest biodiesel yield was up to 78.13%. The characterization of synthesized biodiesel was done through FTIR spectroscopy, (1)H NMR spectra, and gas chromatography.

Show MeSH
Related in: MedlinePlus