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Hydrogen-rich saline protects against ischemia/reperfusion injury in grafts after pancreas transplantations by reducing oxidative stress in rats.

Luo ZL, Cheng L, Ren JD, Fang C, Xiang K, Xu HT, Tang LJ, Wang T, Tian FZ - Mediators Inflamm. (2015)

Bottom Line: In addition, TNF-α, IL-1β, and IL-6 were reduced markedly in the HPT + HS group.Additionally, there were noticeable inhibitory effects on the pancreatic malondialdehyde level and considerable recruitment of SOD and GPx, which are antioxidants.Hydrogen-rich saline treatment significantly attenuated the severity of pancreatic I/R injury in rats, possibly by reducing oxidative stress and inflammation.

View Article: PubMed Central - PubMed

Affiliation: Department of General Surgery, Chengdu Military General Hospital, No. 270, Rongdu Avenue, Jinniu District, Chengdu, Sichuan 610020, China.

ABSTRACT

Purpose: This study aimed to investigate the therapeutic potential of hydrogen-rich saline on pancreatic ischemia/reperfusion (I/R) injury in rats.

Methods: Eighty heterotopic pancreas transplantations (HPT) were performed in syngenic rats. The receptors were randomized blindly into the following three groups: the HPT group and two groups that underwent transplantation and administration of hydrogen-rich saline (HS, >0.6 mM, 6 mL/kg) or normal saline (NS, 6 mL/kg) via the tail vein at the beginning of reperfusion (HPT + HS group, HPT + NS group). Samples from the pancreas and blood were taken at 12 hours after reperfusion. The protective effects of hydrogen-rich saline against I/R injury were evaluated by determining the changes in histopathology and measuring serological parameters, oxidative stress-associated molecules, and proinflammatory cytokines.

Results: Administration of hydrogen-rich saline produced notable protection against pancreatic I/R injury in rats. Histopathological improvements and recovery of impaired pancreatic function were observed. In addition, TNF-α, IL-1β, and IL-6 were reduced markedly in the HPT + HS group. Additionally, there were noticeable inhibitory effects on the pancreatic malondialdehyde level and considerable recruitment of SOD and GPx, which are antioxidants.

Conclusion: Hydrogen-rich saline treatment significantly attenuated the severity of pancreatic I/R injury in rats, possibly by reducing oxidative stress and inflammation.

No MeSH data available.


Related in: MedlinePlus

Inhibition of hydrogen-rich saline treatment on serum cytokine levels in rats. The serum TNF-α (a), IL-1β (b), and IL-6 (c) levels increased strongly after I/R injury. The administration of hydrogen-rich saline (0.6 mM, 6 mL/kg) resulted in significantly decreased levels of serum TNF-α, IL-1β, and IL-6. *P < 0.05 compared with the SO group; #P < 0.05, compared with the HPT + NS group.
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fig3: Inhibition of hydrogen-rich saline treatment on serum cytokine levels in rats. The serum TNF-α (a), IL-1β (b), and IL-6 (c) levels increased strongly after I/R injury. The administration of hydrogen-rich saline (0.6 mM, 6 mL/kg) resulted in significantly decreased levels of serum TNF-α, IL-1β, and IL-6. *P < 0.05 compared with the SO group; #P < 0.05, compared with the HPT + NS group.

Mentions: Compared with the SO group, the levels of serum TNF-α, IL-1β, and IL-6 were remarkably increased in the HPT group, HPT + NS group, and HPT + HS group (P < 0.05). In addition, treatment with hydrogen-rich saline partially reversed those parameters of serum cytokines; the levels were significantly lower than the levels after treatment with normal saline (Figure 3). Our results reveal that treatment with hydrogen-rich saline attenuates I/R injury-induced inflammation in the pancreas.


Hydrogen-rich saline protects against ischemia/reperfusion injury in grafts after pancreas transplantations by reducing oxidative stress in rats.

Luo ZL, Cheng L, Ren JD, Fang C, Xiang K, Xu HT, Tang LJ, Wang T, Tian FZ - Mediators Inflamm. (2015)

Inhibition of hydrogen-rich saline treatment on serum cytokine levels in rats. The serum TNF-α (a), IL-1β (b), and IL-6 (c) levels increased strongly after I/R injury. The administration of hydrogen-rich saline (0.6 mM, 6 mL/kg) resulted in significantly decreased levels of serum TNF-α, IL-1β, and IL-6. *P < 0.05 compared with the SO group; #P < 0.05, compared with the HPT + NS group.
© Copyright Policy - open-access
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4385641&req=5

fig3: Inhibition of hydrogen-rich saline treatment on serum cytokine levels in rats. The serum TNF-α (a), IL-1β (b), and IL-6 (c) levels increased strongly after I/R injury. The administration of hydrogen-rich saline (0.6 mM, 6 mL/kg) resulted in significantly decreased levels of serum TNF-α, IL-1β, and IL-6. *P < 0.05 compared with the SO group; #P < 0.05, compared with the HPT + NS group.
Mentions: Compared with the SO group, the levels of serum TNF-α, IL-1β, and IL-6 were remarkably increased in the HPT group, HPT + NS group, and HPT + HS group (P < 0.05). In addition, treatment with hydrogen-rich saline partially reversed those parameters of serum cytokines; the levels were significantly lower than the levels after treatment with normal saline (Figure 3). Our results reveal that treatment with hydrogen-rich saline attenuates I/R injury-induced inflammation in the pancreas.

Bottom Line: In addition, TNF-α, IL-1β, and IL-6 were reduced markedly in the HPT + HS group.Additionally, there were noticeable inhibitory effects on the pancreatic malondialdehyde level and considerable recruitment of SOD and GPx, which are antioxidants.Hydrogen-rich saline treatment significantly attenuated the severity of pancreatic I/R injury in rats, possibly by reducing oxidative stress and inflammation.

View Article: PubMed Central - PubMed

Affiliation: Department of General Surgery, Chengdu Military General Hospital, No. 270, Rongdu Avenue, Jinniu District, Chengdu, Sichuan 610020, China.

ABSTRACT

Purpose: This study aimed to investigate the therapeutic potential of hydrogen-rich saline on pancreatic ischemia/reperfusion (I/R) injury in rats.

Methods: Eighty heterotopic pancreas transplantations (HPT) were performed in syngenic rats. The receptors were randomized blindly into the following three groups: the HPT group and two groups that underwent transplantation and administration of hydrogen-rich saline (HS, >0.6 mM, 6 mL/kg) or normal saline (NS, 6 mL/kg) via the tail vein at the beginning of reperfusion (HPT + HS group, HPT + NS group). Samples from the pancreas and blood were taken at 12 hours after reperfusion. The protective effects of hydrogen-rich saline against I/R injury were evaluated by determining the changes in histopathology and measuring serological parameters, oxidative stress-associated molecules, and proinflammatory cytokines.

Results: Administration of hydrogen-rich saline produced notable protection against pancreatic I/R injury in rats. Histopathological improvements and recovery of impaired pancreatic function were observed. In addition, TNF-α, IL-1β, and IL-6 were reduced markedly in the HPT + HS group. Additionally, there were noticeable inhibitory effects on the pancreatic malondialdehyde level and considerable recruitment of SOD and GPx, which are antioxidants.

Conclusion: Hydrogen-rich saline treatment significantly attenuated the severity of pancreatic I/R injury in rats, possibly by reducing oxidative stress and inflammation.

No MeSH data available.


Related in: MedlinePlus