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Hydrogen-rich saline protects against ischemia/reperfusion injury in grafts after pancreas transplantations by reducing oxidative stress in rats.

Luo ZL, Cheng L, Ren JD, Fang C, Xiang K, Xu HT, Tang LJ, Wang T, Tian FZ - Mediators Inflamm. (2015)

Bottom Line: In addition, TNF-α, IL-1β, and IL-6 were reduced markedly in the HPT + HS group.Additionally, there were noticeable inhibitory effects on the pancreatic malondialdehyde level and considerable recruitment of SOD and GPx, which are antioxidants.Hydrogen-rich saline treatment significantly attenuated the severity of pancreatic I/R injury in rats, possibly by reducing oxidative stress and inflammation.

View Article: PubMed Central - PubMed

Affiliation: Department of General Surgery, Chengdu Military General Hospital, No. 270, Rongdu Avenue, Jinniu District, Chengdu, Sichuan 610020, China.

ABSTRACT

Purpose: This study aimed to investigate the therapeutic potential of hydrogen-rich saline on pancreatic ischemia/reperfusion (I/R) injury in rats.

Methods: Eighty heterotopic pancreas transplantations (HPT) were performed in syngenic rats. The receptors were randomized blindly into the following three groups: the HPT group and two groups that underwent transplantation and administration of hydrogen-rich saline (HS, >0.6 mM, 6 mL/kg) or normal saline (NS, 6 mL/kg) via the tail vein at the beginning of reperfusion (HPT + HS group, HPT + NS group). Samples from the pancreas and blood were taken at 12 hours after reperfusion. The protective effects of hydrogen-rich saline against I/R injury were evaluated by determining the changes in histopathology and measuring serological parameters, oxidative stress-associated molecules, and proinflammatory cytokines.

Results: Administration of hydrogen-rich saline produced notable protection against pancreatic I/R injury in rats. Histopathological improvements and recovery of impaired pancreatic function were observed. In addition, TNF-α, IL-1β, and IL-6 were reduced markedly in the HPT + HS group. Additionally, there were noticeable inhibitory effects on the pancreatic malondialdehyde level and considerable recruitment of SOD and GPx, which are antioxidants.

Conclusion: Hydrogen-rich saline treatment significantly attenuated the severity of pancreatic I/R injury in rats, possibly by reducing oxidative stress and inflammation.

No MeSH data available.


Related in: MedlinePlus

Changes in insulin (a), amylase (b), and lipase (c) serum concentrations in rats exposed to various treatments. As with the results of oxidative stress after I/R injury, the levels of insulin were decreased, whereas the levels of amylase and lipase were increased. The treatment with hydrogen-rich saline reverted those parameters to basal levels. *P < 0.05 compared with the SO group; #P < 0.05 compared with the HPT + NS group.
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fig2: Changes in insulin (a), amylase (b), and lipase (c) serum concentrations in rats exposed to various treatments. As with the results of oxidative stress after I/R injury, the levels of insulin were decreased, whereas the levels of amylase and lipase were increased. The treatment with hydrogen-rich saline reverted those parameters to basal levels. *P < 0.05 compared with the SO group; #P < 0.05 compared with the HPT + NS group.

Mentions: I/R induced a significant reduction in the insulin concentration in the serum (P < 0.05) (Figure 2(a)). The administration of hydrogen-rich saline partially restored the level of insulin in the HPT + HS group and caused a noticeable increase compared with the HPT + NS group. There were marked increases in the amylase and lipase levels in the serum of rats subjected to I/R injury in the HPT group, HPT + NS group, and HPT + HS group compared with the SO group (P < 0.05) (Figures 2(b) and 2(c)). Hydrogen-rich saline administration significantly reduced the amylase and lipase levels in the HPT + HS group compared with those in the HPT + NS group (P < 0.05). These results indicated that the hydrogen-rich saline might be helpful for the recovery of endocrine function of pancreas and it reduced the severity of graft pancreatitis.


Hydrogen-rich saline protects against ischemia/reperfusion injury in grafts after pancreas transplantations by reducing oxidative stress in rats.

Luo ZL, Cheng L, Ren JD, Fang C, Xiang K, Xu HT, Tang LJ, Wang T, Tian FZ - Mediators Inflamm. (2015)

Changes in insulin (a), amylase (b), and lipase (c) serum concentrations in rats exposed to various treatments. As with the results of oxidative stress after I/R injury, the levels of insulin were decreased, whereas the levels of amylase and lipase were increased. The treatment with hydrogen-rich saline reverted those parameters to basal levels. *P < 0.05 compared with the SO group; #P < 0.05 compared with the HPT + NS group.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4385641&req=5

fig2: Changes in insulin (a), amylase (b), and lipase (c) serum concentrations in rats exposed to various treatments. As with the results of oxidative stress after I/R injury, the levels of insulin were decreased, whereas the levels of amylase and lipase were increased. The treatment with hydrogen-rich saline reverted those parameters to basal levels. *P < 0.05 compared with the SO group; #P < 0.05 compared with the HPT + NS group.
Mentions: I/R induced a significant reduction in the insulin concentration in the serum (P < 0.05) (Figure 2(a)). The administration of hydrogen-rich saline partially restored the level of insulin in the HPT + HS group and caused a noticeable increase compared with the HPT + NS group. There were marked increases in the amylase and lipase levels in the serum of rats subjected to I/R injury in the HPT group, HPT + NS group, and HPT + HS group compared with the SO group (P < 0.05) (Figures 2(b) and 2(c)). Hydrogen-rich saline administration significantly reduced the amylase and lipase levels in the HPT + HS group compared with those in the HPT + NS group (P < 0.05). These results indicated that the hydrogen-rich saline might be helpful for the recovery of endocrine function of pancreas and it reduced the severity of graft pancreatitis.

Bottom Line: In addition, TNF-α, IL-1β, and IL-6 were reduced markedly in the HPT + HS group.Additionally, there were noticeable inhibitory effects on the pancreatic malondialdehyde level and considerable recruitment of SOD and GPx, which are antioxidants.Hydrogen-rich saline treatment significantly attenuated the severity of pancreatic I/R injury in rats, possibly by reducing oxidative stress and inflammation.

View Article: PubMed Central - PubMed

Affiliation: Department of General Surgery, Chengdu Military General Hospital, No. 270, Rongdu Avenue, Jinniu District, Chengdu, Sichuan 610020, China.

ABSTRACT

Purpose: This study aimed to investigate the therapeutic potential of hydrogen-rich saline on pancreatic ischemia/reperfusion (I/R) injury in rats.

Methods: Eighty heterotopic pancreas transplantations (HPT) were performed in syngenic rats. The receptors were randomized blindly into the following three groups: the HPT group and two groups that underwent transplantation and administration of hydrogen-rich saline (HS, >0.6 mM, 6 mL/kg) or normal saline (NS, 6 mL/kg) via the tail vein at the beginning of reperfusion (HPT + HS group, HPT + NS group). Samples from the pancreas and blood were taken at 12 hours after reperfusion. The protective effects of hydrogen-rich saline against I/R injury were evaluated by determining the changes in histopathology and measuring serological parameters, oxidative stress-associated molecules, and proinflammatory cytokines.

Results: Administration of hydrogen-rich saline produced notable protection against pancreatic I/R injury in rats. Histopathological improvements and recovery of impaired pancreatic function were observed. In addition, TNF-α, IL-1β, and IL-6 were reduced markedly in the HPT + HS group. Additionally, there were noticeable inhibitory effects on the pancreatic malondialdehyde level and considerable recruitment of SOD and GPx, which are antioxidants.

Conclusion: Hydrogen-rich saline treatment significantly attenuated the severity of pancreatic I/R injury in rats, possibly by reducing oxidative stress and inflammation.

No MeSH data available.


Related in: MedlinePlus