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Hydrogen-rich saline protects against ischemia/reperfusion injury in grafts after pancreas transplantations by reducing oxidative stress in rats.

Luo ZL, Cheng L, Ren JD, Fang C, Xiang K, Xu HT, Tang LJ, Wang T, Tian FZ - Mediators Inflamm. (2015)

Bottom Line: In addition, TNF-α, IL-1β, and IL-6 were reduced markedly in the HPT + HS group.Additionally, there were noticeable inhibitory effects on the pancreatic malondialdehyde level and considerable recruitment of SOD and GPx, which are antioxidants.Hydrogen-rich saline treatment significantly attenuated the severity of pancreatic I/R injury in rats, possibly by reducing oxidative stress and inflammation.

View Article: PubMed Central - PubMed

Affiliation: Department of General Surgery, Chengdu Military General Hospital, No. 270, Rongdu Avenue, Jinniu District, Chengdu, Sichuan 610020, China.

ABSTRACT

Purpose: This study aimed to investigate the therapeutic potential of hydrogen-rich saline on pancreatic ischemia/reperfusion (I/R) injury in rats.

Methods: Eighty heterotopic pancreas transplantations (HPT) were performed in syngenic rats. The receptors were randomized blindly into the following three groups: the HPT group and two groups that underwent transplantation and administration of hydrogen-rich saline (HS, >0.6 mM, 6 mL/kg) or normal saline (NS, 6 mL/kg) via the tail vein at the beginning of reperfusion (HPT + HS group, HPT + NS group). Samples from the pancreas and blood were taken at 12 hours after reperfusion. The protective effects of hydrogen-rich saline against I/R injury were evaluated by determining the changes in histopathology and measuring serological parameters, oxidative stress-associated molecules, and proinflammatory cytokines.

Results: Administration of hydrogen-rich saline produced notable protection against pancreatic I/R injury in rats. Histopathological improvements and recovery of impaired pancreatic function were observed. In addition, TNF-α, IL-1β, and IL-6 were reduced markedly in the HPT + HS group. Additionally, there were noticeable inhibitory effects on the pancreatic malondialdehyde level and considerable recruitment of SOD and GPx, which are antioxidants.

Conclusion: Hydrogen-rich saline treatment significantly attenuated the severity of pancreatic I/R injury in rats, possibly by reducing oxidative stress and inflammation.

No MeSH data available.


Related in: MedlinePlus

Histopathologic analysis of the pancreas using hematoxylin-eosin staining (×200). (a) SO group: pancreatic acini are well conserved without edema, inflammatory infiltrates, or hemorrhage. ((b), (c)) HPT and HPT + NS groups: acute pancreatitis characterized by interacinar edema, inflammatory infiltrates, necrosis, and hemorrhage. (d) HPT + HS group: these histological changes showed significantly less extensive inflammatory infiltrates, interacinar edema, necrosis, and hemorrhage. Bar equals 50 μm.
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fig1: Histopathologic analysis of the pancreas using hematoxylin-eosin staining (×200). (a) SO group: pancreatic acini are well conserved without edema, inflammatory infiltrates, or hemorrhage. ((b), (c)) HPT and HPT + NS groups: acute pancreatitis characterized by interacinar edema, inflammatory infiltrates, necrosis, and hemorrhage. (d) HPT + HS group: these histological changes showed significantly less extensive inflammatory infiltrates, interacinar edema, necrosis, and hemorrhage. Bar equals 50 μm.

Mentions: Histopathological examination of the pancreas demonstrated that all of the animals undergoing transplantation showed signs of a postischemia pancreatitis with significant edema formation, inflammatory infiltration, and cell necrosis. In the HPT + HS group, these histological changes were significantly less evident compared with the animals that received normal saline before reperfusion, as evidenced by the histopathological scoring (Table 1) (Figure 1). These results showed that the hydrogen-rich saline might relate to the relieving of histopathological changes of pancreatic tissue.


Hydrogen-rich saline protects against ischemia/reperfusion injury in grafts after pancreas transplantations by reducing oxidative stress in rats.

Luo ZL, Cheng L, Ren JD, Fang C, Xiang K, Xu HT, Tang LJ, Wang T, Tian FZ - Mediators Inflamm. (2015)

Histopathologic analysis of the pancreas using hematoxylin-eosin staining (×200). (a) SO group: pancreatic acini are well conserved without edema, inflammatory infiltrates, or hemorrhage. ((b), (c)) HPT and HPT + NS groups: acute pancreatitis characterized by interacinar edema, inflammatory infiltrates, necrosis, and hemorrhage. (d) HPT + HS group: these histological changes showed significantly less extensive inflammatory infiltrates, interacinar edema, necrosis, and hemorrhage. Bar equals 50 μm.
© Copyright Policy - open-access
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4385641&req=5

fig1: Histopathologic analysis of the pancreas using hematoxylin-eosin staining (×200). (a) SO group: pancreatic acini are well conserved without edema, inflammatory infiltrates, or hemorrhage. ((b), (c)) HPT and HPT + NS groups: acute pancreatitis characterized by interacinar edema, inflammatory infiltrates, necrosis, and hemorrhage. (d) HPT + HS group: these histological changes showed significantly less extensive inflammatory infiltrates, interacinar edema, necrosis, and hemorrhage. Bar equals 50 μm.
Mentions: Histopathological examination of the pancreas demonstrated that all of the animals undergoing transplantation showed signs of a postischemia pancreatitis with significant edema formation, inflammatory infiltration, and cell necrosis. In the HPT + HS group, these histological changes were significantly less evident compared with the animals that received normal saline before reperfusion, as evidenced by the histopathological scoring (Table 1) (Figure 1). These results showed that the hydrogen-rich saline might relate to the relieving of histopathological changes of pancreatic tissue.

Bottom Line: In addition, TNF-α, IL-1β, and IL-6 were reduced markedly in the HPT + HS group.Additionally, there were noticeable inhibitory effects on the pancreatic malondialdehyde level and considerable recruitment of SOD and GPx, which are antioxidants.Hydrogen-rich saline treatment significantly attenuated the severity of pancreatic I/R injury in rats, possibly by reducing oxidative stress and inflammation.

View Article: PubMed Central - PubMed

Affiliation: Department of General Surgery, Chengdu Military General Hospital, No. 270, Rongdu Avenue, Jinniu District, Chengdu, Sichuan 610020, China.

ABSTRACT

Purpose: This study aimed to investigate the therapeutic potential of hydrogen-rich saline on pancreatic ischemia/reperfusion (I/R) injury in rats.

Methods: Eighty heterotopic pancreas transplantations (HPT) were performed in syngenic rats. The receptors were randomized blindly into the following three groups: the HPT group and two groups that underwent transplantation and administration of hydrogen-rich saline (HS, >0.6 mM, 6 mL/kg) or normal saline (NS, 6 mL/kg) via the tail vein at the beginning of reperfusion (HPT + HS group, HPT + NS group). Samples from the pancreas and blood were taken at 12 hours after reperfusion. The protective effects of hydrogen-rich saline against I/R injury were evaluated by determining the changes in histopathology and measuring serological parameters, oxidative stress-associated molecules, and proinflammatory cytokines.

Results: Administration of hydrogen-rich saline produced notable protection against pancreatic I/R injury in rats. Histopathological improvements and recovery of impaired pancreatic function were observed. In addition, TNF-α, IL-1β, and IL-6 were reduced markedly in the HPT + HS group. Additionally, there were noticeable inhibitory effects on the pancreatic malondialdehyde level and considerable recruitment of SOD and GPx, which are antioxidants.

Conclusion: Hydrogen-rich saline treatment significantly attenuated the severity of pancreatic I/R injury in rats, possibly by reducing oxidative stress and inflammation.

No MeSH data available.


Related in: MedlinePlus